LOCUS SIU01667.1 437 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 ppe family protein ppe46 protein. ACCESSION LT708304-3081 PROTEIN_ID SIU01667.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="PPE46" /locus_tag="BQ2027_MB3043C" /note="Mb3043c, PPE46, len: 437 aa. Equivalent to Rv3018c, len: 434 aa, from Mycobacterium tuberculosis strain H37Rv, (99.5% identity in 431 aa overlap). Member of PPE family but lacks Gly, Ala rich repeats at C-terminal domain, closest to MTCY261.19. Also very similar to following ORF MTV012.35c. Nearly identical in parts to Mycobacterium tuberculosis protein erroneously described as DIHYDROFOLATE REDUCTASE (X59271|MTFOLA_1) P31500|DYR_MYCTU (214 aa), FASTA scores: opt: 972, E(): 4.4e-42, (80.0% identity in 195 aa overlap); and Z97559|MTCY261_19 from M. tuberculosis cosmid (473 aa), FASTA scores: opt: 806, E(): 0; (38.8% identity in 479 aa overlap); and O53268|MTV012.35c from Mycobacterium tuberculosis (358 aa), FASTA scores: opt: 1714, E(): 3.3e-79, (78.3% identity in 355 aa overlap). REMARK-M.bovis-M.tuberculosis: In Mycobacterium bovis, a 56 bp deletion leads to a product slightly different at the Nh2-terminus part compared to its homolog in Mycobacterium tuberculosis strain H37Rv (434 aa versus 437 aa)." /db_xref="GOA:A0A1R3Y315" /db_xref="InterPro:IPR000030" /db_xref="InterPro:IPR038332" /db_xref="UniProtKB/TrEMBL:A0A1R3Y315" BEGIN 1 MSSHRTPVWL ASPPEVHSAL LSAGPGPGSL QAAAAGWSAL SAEYAAVAQE LSAVVAAVGA 61 GVWQGPSAEL FVAAYVPYVA WLVQASADSA AAAGEHEAAA AGYVCALAEM PTLPELAANH 121 LTHAVLVATN FFGINTIPIA LNEADYVRMW VQAATVMSAY EAVVGAALVA TPHTGPAPVI 181 VKPGANEASN AVAAATITPF PWHEIVQFLE ETFAAYDQYL SALLSELPAV AWVWFQLFVD 241 ILGFNIIGFI ITLASNAQLL TEFAINASYV AVGLLYAIAG VIDIVVEWVI GNLFGVVPLL 301 GGPLLGALAA AVVPGVAGLA GVAGLAAVPA VGAAAGAPAA LVGSVAPVSG GVVSPQARLV 361 SAVEPAPAST SVSVLASDRG AGALGFVGTA GKESVGQPAG LTVLADEFGD GAPVPMLPGS 421 WGPDLVGVAG DGGLVSV //