LOCUS SIU01654.1 279 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 Membrane protein 2, distant similarity to thiosulphate:quinone oxidoreductase DoxD protein. ACCESSION LT708304-3068 PROTEIN_ID SIU01654.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB3030C" /note="Mb3030c, -, len: 279 aa. Equivalent to Rv3005c, len: 279 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 279 aa overlap). Conserved hypothetical protein, equivalent to O33110|MLCB637.18|ML1698 HYPOTHETICAL 29.5 KDA PROTEIN from Mycobacterium leprae (277 aa), FASTA scores: opt: 1245, E(): 1.2e-65, (70.5% identity in 278 aa overlap). Also similar, but longer 100 aa in N-terminus, to other hypothetical proteins, few membrane proteins, e.g. Q9RKN9|SCC75A.35 PUTATIVE MEMBRANE PROTEIN from Streptomyces coelicolor (180 aa), FASTA scores: opt: 326, E(): 3.9e-12, (44.2% identity in 138 aa overlap); P96694|YDFP|AB001488 HYPOTHETICAL PROTEIN from Bacillus subtilis (129 aa), FASTA scores: opt:273, E(): 3.7e-09, (33.1% identity in 130 aa overlap); Q9KKT1|VCA1019 HYPOTHETICAL PROTEIN from Vibrio cholerae (148 aa), FASTA scores: opt: 258, E(): 3.1e-08, (34.9% identity in 126 aa overlap); etc. Mb3030c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y2V3" /db_xref="InterPro:IPR032808" /db_xref="UniProtKB/TrEMBL:A0A1R3Y2V3" BEGIN 1 MTSSNDSHWQ RPDDSPGPMP GRPVSASLVD PEDDLTPARY AGDFGSGTTT VIPPYDAASS 61 GVGNSGYSLI EAAEPLPYVQ PQPGRQVPAG SAGIDMDDDE RVRAAGRRGT QNLGLLILRV 121 GLGAVLIAHG LQKLFGWWDG QGLAGFQNSL SDIGYQHAEI LAYVSAGGEI VAGVLLVLGL 181 FTPLAAAGAL AFLINGLLAG ISAQHSRPVA YFLQDGHEYQ ITLVVMAVAV ILSGPGRYGL 241 DAARGWAHRP FIGSFVALLG GIAAGIAVWV LLNGANPLA //