LOCUS SIU01652.1 618 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 acetolactate synthase (large subunit) ilvb1 (acetohydroxy-acid synthase) protein. ACCESSION LT708304-3066 PROTEIN_ID SIU01652.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="ilvB1" /locus_tag="BQ2027_MB3028C" /standard_name="ilvB" /note="Mb3028c, ilvB1, len: 618 aa. Equivalent to Rv3003c, len: 618 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 618 aa overlap). Probable ilvB1, acetolactate synthase, large subunit (EC 4.1.3.18), equivalent or highly similar to others e.g. O33112|ILVB_MYCLE|MLCB637.20|ML1696 from Mycobacterium leprae (625 aa), FASTA scores: opt: 3653, E(): 5.4e-208, (87.1% identity in 627 aa overlap); Q59498|ILVB_MYCAV from Mycobacterium avium (621 aa), FASTA scores: opt: 3473, E(): 2.3e-197, (84.7% identity in 614 aa overlap); P42463|ILVB_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (626 aa), FASTA scores: opt: 2754, E(): 5.9e-155, (65.8% identity in 589 aa overlap); etc. Contains PS00187 Thiamine pyrophosphate enzymes signature. COFACTOR: THIAMINE PYROPHOSPHATE, AND MAGNESIUM (BY SIMILARITY). Note that previously known as ilvB. Protein product from Mb3028c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3028c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:P0A623" /db_xref="InterPro:IPR000399" /db_xref="InterPro:IPR011766" /db_xref="InterPro:IPR012000" /db_xref="InterPro:IPR012001" /db_xref="InterPro:IPR012846" /db_xref="InterPro:IPR029035" /db_xref="InterPro:IPR029061" /db_xref="InterPro:IPR039368" /db_xref="UniProtKB/Swiss-Prot:P0A623" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MSAPTKPHSP TFKPEPHSAA NEPKHPAARP KHVALQQLTG AQAVIRSLEE LGVDVIFGIP 61 GGAVLPVYDP LFDSKKLRHV LVRHEQGAGH AASGYAHVTG RVGVCMATSG PGATNLVTPL 121 ADAQMDSIPV VAITGQVGRG LIGTDAFQEA DISGITMPIT KHNFLVRSGD DIPRVLAEAF 181 HIAASGRPGA VLVDIPKDVL QGQCTFSWPP RMELPGYKPN TKPHSRQVRE AAKLIAAARK 241 PVLYVGGGVI RGEATEQLRE LAELTGIPVV TTLMARGAFP DSHRQNLGMP GMHGTVAAVA 301 ALQRSDLLIA LGTRFDDRVT GKLDSFAPEA KVIHADIDPA EIGKNRHADV PIVGDVKAVI 361 TELIAMLRHH HIPGTIEMAD WWAYLNGVRK TYPLSYGPQS DGSLSPEYVI EKLGEIAGPD 421 AVFVAGVGQH QMWAAQFIRY EKPRSWLNSG GLGTMGFAIP AAMGAKIALP GTEVWAIDGD 481 GCFQMTNQEL ATCAVEGIPV KVALINNGNL GMVRQWQSLF YAERYSQTDL ATHSHRIPDF 541 VKLAEALGCV GLRCEREEDV VDVINQARAI NDCPVVIDFI VGADAQVWPM VAAGTSNDEI 601 QAARGIRPLF DDITEGHA //