LOCUS SIU01641.1 239 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE 2-HYDROXYHEPTA-2,4-DIENE-1,7-DIOATE ISOMERASE (HHDD ISOMERASE) protein. ACCESSION LT708304-3055 PROTEIN_ID SIU01641.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB3017C" /note="Mb3017c, -, len: 239 aa. Equivalent to Rv2993c, len: 239 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 239 aa overlap). Possible 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), equivalent to O33119|ML1689|MLCB637.28 POSSIBLE 2-HYDROXYHEPTA-2,4-DIENE- 1,7-DIOATE ISOMERASE from Mycobacterium leprae (242 aa), FASTA scores: opt: 1427, E(): 4.4e-86, (85.9% identity in 241 aa overlap). Also similar to others e.g. Q9LBE3|DR1609 from Deinococcus radiodurans (250 aa), FASTA scores: opt: 723, E(): 5.5e-40, (49.05% identity in 216 aa overlap); O27551|MTH1507 from Methanothermobacter thermautotrophicus (260 aa), FASTA scores: opt: 708, E(): 5.4e-39, (52.1% identity in 213 aa overlap); Q9HQR6|VNG1037G|HPCE from Halobacterium sp. (strain NRC-1) (244 aa), FASTA scores: opt: 590, E(): 2.7e-31, (43.65% identity in 220 aa overlap); etc. Start chosen by homology, but ORF could continue upstream. TBparse score is 0.896. Protein product from Mb3017c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3017c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y2T1" /db_xref="InterPro:IPR011234" /db_xref="InterPro:IPR018833" /db_xref="InterPro:IPR036663" /db_xref="UniProtKB/TrEMBL:A0A1R3Y2T1" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MTAREIAEHP FGTPTFTGRS WPLADVRLLA PILASKVVCV GKNYADHIAE MGGRPPADPV 61 IFLKPNTAII GPNTPIRLPA NASPVHFEGE LAIVIGRACK DVPAAQAVDN ILGYTIGNDV 121 SARDQQQSDG QWTRAKGHDT FCPVGPWIVT DLAPFDPADL ELRTVVNGDV KQHARTSLMI 181 HDIGAIVEWI SAIMTLLPGD LILTGTPAGV GPIEDGDTVS ITIEGIGTLT NPVVRKGKP //