LOCUS       SIU01636.1               473 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            3-ISOPROPYLMALATE DEHYDRATASE (LARGE SUBUNIT) LEUC (ISOPROPYLMALATE 
            ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI) protein.
ACCESSION   LT708304-3050
PROTEIN_ID  SIU01636.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="leuC"
                     /locus_tag="BQ2027_MB3012C"
                     /note="Mb3012c, leuC, len: 473 aa. Equivalent to Rv2988c,
                     len: 473 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (99.8% identity in 473 aa overlap). Probable leuC,
                     3-isopropylmalate dehydratase, large subunit (EC
                     4.2.1.33), equivalent to O33123|LEU2_MYCLE
                     3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT from
                     Mycobacterium leprae (476 aa), FASTA scores: opt: 2818,
                     E(): 1.3e-171, (88.75% identity in 471 aa overlap). Also
                     highly similar to many e.g. Q44427|LEU2_ACTTI from
                     Actinoplanes teichomyceticus (485 aa), FASTA scores: opt:
                     1958, E(): 6.5e-117, (71.0% identity in 479 aa overlap);
                     P55251|LEU2_RHIPU from Rhizomucor pusillus (755 aa), FASTA
                     scores: opt: 1937, E(): 1.9e-115, (61.25% identity in 467
                     aa overlap) (C-terminus longer);
                     P30127|LEU2_ECOLI|LEUC|B0072 from Escherichia coli strain
                     K12 (465 aa), FASTA scores: opt: 1896, E(): 5.5e-113,
                     (61.6% identity in 456 aa overlap); etc. Contains PS00450
                     Aconitase family signature. BELONGS TO THE ACONITASE/IPM
                     ISOMERASE FAMILY. TBparse score is 0.895. Protein product
                     from Mb3012c detected using shotgun mass spectrometry and
                     SWATH mass spectrometry. Mb3012c found to be expressed
                     during exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:Q7TXH6"
                     /db_xref="InterPro:IPR001030"
                     /db_xref="InterPro:IPR004430"
                     /db_xref="InterPro:IPR015931"
                     /db_xref="InterPro:IPR018136"
                     /db_xref="InterPro:IPR033941"
                     /db_xref="InterPro:IPR036008"
                     /db_xref="UniProtKB/Swiss-Prot:Q7TXH6"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MALQTGEPRT LAEKIWDDHI VVSGGGCAPD LIYIDLHLVH EVTSPQAFDG LRLAGRRVRR
       61 PELTLATEDH NVPTVDIDQP IADPVSRTQV ETLRRNCAEF GIRLHSMGDI EQGIVHVVGP
      121 QLGLTQPGMT IVCGDSHTST HGAFGALAMG IGTSEVEHVL ATQTLPLRPF KTMAVNVDGR
      181 LPDGVSAKDI ILALIAKIGT GGGQGHVIEY RGSAIESLSM EGRMTICNMS IEAGARAGMV
      241 APDETTYAFL RGRPHAPTGA QWDTALVYWQ RLRTDVGAVF DTEVYLDAAS LSPFVTWGTN
      301 PGQGVPLAAA VPDPQLMTDD AERQAAEKAL AYMDLRPGTA MREIAVDAVF VGSCTNGRIE
      361 DLRVVAEVLR GRKVADGVRM LIVPGSMRVR AQAEAEGLGE IFTDAGAQWR QAGCSMCLGM
      421 NPDQLASGER CAATSNRNFE GRQGAGGRTH LVSPAVAAAT AVRGTLSSPA DLN
//