LOCUS SIU01636.1 473 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE 3-ISOPROPYLMALATE DEHYDRATASE (LARGE SUBUNIT) LEUC (ISOPROPYLMALATE ISOMERASE) (ALPHA-IPM ISOMERASE) (IPMI) protein. ACCESSION LT708304-3050 PROTEIN_ID SIU01636.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="leuC" /locus_tag="BQ2027_MB3012C" /note="Mb3012c, leuC, len: 473 aa. Equivalent to Rv2988c, len: 473 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 473 aa overlap). Probable leuC, 3-isopropylmalate dehydratase, large subunit (EC 4.2.1.33), equivalent to O33123|LEU2_MYCLE 3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT from Mycobacterium leprae (476 aa), FASTA scores: opt: 2818, E(): 1.3e-171, (88.75% identity in 471 aa overlap). Also highly similar to many e.g. Q44427|LEU2_ACTTI from Actinoplanes teichomyceticus (485 aa), FASTA scores: opt: 1958, E(): 6.5e-117, (71.0% identity in 479 aa overlap); P55251|LEU2_RHIPU from Rhizomucor pusillus (755 aa), FASTA scores: opt: 1937, E(): 1.9e-115, (61.25% identity in 467 aa overlap) (C-terminus longer); P30127|LEU2_ECOLI|LEUC|B0072 from Escherichia coli strain K12 (465 aa), FASTA scores: opt: 1896, E(): 5.5e-113, (61.6% identity in 456 aa overlap); etc. Contains PS00450 Aconitase family signature. BELONGS TO THE ACONITASE/IPM ISOMERASE FAMILY. TBparse score is 0.895. Protein product from Mb3012c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3012c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:Q7TXH6" /db_xref="InterPro:IPR001030" /db_xref="InterPro:IPR004430" /db_xref="InterPro:IPR015931" /db_xref="InterPro:IPR018136" /db_xref="InterPro:IPR033941" /db_xref="InterPro:IPR036008" /db_xref="UniProtKB/Swiss-Prot:Q7TXH6" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MALQTGEPRT LAEKIWDDHI VVSGGGCAPD LIYIDLHLVH EVTSPQAFDG LRLAGRRVRR 61 PELTLATEDH NVPTVDIDQP IADPVSRTQV ETLRRNCAEF GIRLHSMGDI EQGIVHVVGP 121 QLGLTQPGMT IVCGDSHTST HGAFGALAMG IGTSEVEHVL ATQTLPLRPF KTMAVNVDGR 181 LPDGVSAKDI ILALIAKIGT GGGQGHVIEY RGSAIESLSM EGRMTICNMS IEAGARAGMV 241 APDETTYAFL RGRPHAPTGA QWDTALVYWQ RLRTDVGAVF DTEVYLDAAS LSPFVTWGTN 301 PGQGVPLAAA VPDPQLMTDD AERQAAEKAL AYMDLRPGTA MREIAVDAVF VGSCTNGRIE 361 DLRVVAEVLR GRKVADGVRM LIVPGSMRVR AQAEAEGLGE IFTDAGAQWR QAGCSMCLGM 421 NPDQLASGER CAATSNRNFE GRQGAGGRTH LVSPAVAAAT AVRGTLSSPA DLN //