LOCUS SIU01632.1 742 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 polyphosphate kinase ppk (polyphosphoric acid kinase) (atp-polyphosphate phosphotransferase) protein. ACCESSION LT708304-3046 PROTEIN_ID SIU01632.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="ppk" /locus_tag="BQ2027_MB3008" /note="Mb3008, ppk, len: 742 aa. Equivalent to Rv2984, len: 742 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 742 aa overlap). Probable ppk, polyphosphate kinase (EC 2.7.4.1), equivalent to O33127|PPK_MYCLE POLYPHOSPHATE KINASE from Mycobacterium leprae (739 aa), FASTA scores: opt: 4264, E(): 0, (87.85% identity in 742 aa overlap). Also highly similar to others e.g. Q9KZV6|PPK_STRCO from Streptomyces coelicolor (746 aa), FASTA scores: opt: 1979, E(): 2.6e-117, (59.9% identity in 701 aa overlap); Q9KD27|PPK_BACHD from Bacillus halodurans (705 aa), FASTA scores: opt: 1319, E(): 1.4e-75, (45.55% identity in 674 aa overlap); Q9PAC7|PPK_XYLFA from Xylella fastidiosa (698 aa), FASTA scores: opt: 1300, E(): 2.2e-74, (43.3% identity in 693 aa overlap); etc. BELONGS TO THE POLYPHOSPHATE KINASE FAMILY. Protein product from Mb3008 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3008 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:P65769" /db_xref="InterPro:IPR003414" /db_xref="InterPro:IPR024953" /db_xref="InterPro:IPR025198" /db_xref="InterPro:IPR025200" /db_xref="InterPro:IPR036830" /db_xref="InterPro:IPR036832" /db_xref="InterPro:IPR041108" /db_xref="UniProtKB/Swiss-Prot:P65769" BEGIN 1 MMSNDRKVTE IENSPVTEVR PEEHAWYPDD SALAAPPAAT PAAISDQLPS DRYLNRELSW 61 LDFNARVLAL AADKSMPLLE RAKFLAIFAS NLDEFYMVRV AGLKRRDEMG LSVRSADGLT 121 PREQLGRIGE QTQQLASRHA RVFLDSVLPA LGEEGIYIVT WADLDQAERD RLSTYFNEQV 181 FPVLTPLAVD PAHPFPFVSG LSLNLAVTVR QPEDGTQHFA RVKVPDNVDR FVELAAREAS 241 EEAAGTEGRT ALRFLPMEEL IAAFLPVLFP GMEIVEHHAF RITRNADFEV EEDRDEDLLQ 301 ALERELARRR FGSPVRLEIA DDMTESMLEL LLRELDVHPG DVIEVPGLLD LSSLWQIYAV 361 DRPTLKDRTF VPATHPAFAE RETPKSIFAT LREGDVLVHH PYDSFSTSVQ RFIEQAAADP 421 NVLAIKQTLY RTSGDSPIVR ALIDAAEAGK QVVALVEIKA RFDEQANIAW ARALEQAGVH 481 VAYGLVGLKT HCKTALVVRR EGPTIRRYCH VGTGNYNSKT ARLYEDVGLL TAAPDIGADL 541 TDLFNSLTGY SRKLSYRNLL VAPHGIRAGI IDRVEREVAA HRAEGAHNGK GRIRLKMNAL 601 VDEQVIDALY RASRAGVRIE VVVRGICALR PGAQGISENI IVRSILGRFL EHSRILHFRA 661 IDEFWIGSAD MMHRNLDRRV EVMAQVKNPR LTAQLDELFE SALDPCTRCW ELGPDGQWTA 721 SPQEGHSVRD HQESLMERHR SP //