LOCUS SIU01625.1 333 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE THIAMINE-MONOPHOSPHATE KINASE THIL (THIAMINE-PHOSPHATE KINASE) protein. ACCESSION LT708304-3039 PROTEIN_ID SIU01625.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="thiL" /locus_tag="BQ2027_MB3001C" /note="Mb3001c, thiL, len: 333 aa. Equivalent to Rv2977c, len: 333 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 333 aa overlap). Possible thiL, thiamin-monophosphate kinase (EC), equivalent to Q9CBS2 PROBABLE THIAMINE-MONOPHOSPHATE KINASE from Mycobacterium leprae (325 aa), FASTA scores: opt: 1738, E(): 4.5e-98, (80.9% identity in 314 aa overlap). Also highly similar to others e.g. Q9ZBR7|SC7A1.06 PUTATIVE THIAMINE MONPHOSPHATE KINASE from Streptomyces coelicolor (322 aa), FASTA scores: opt: 959, E(): 7.8e-51, (51.1% identity in 319 aa overlap); O05514|THIL_BACSU THIAMINE-MONOPHOSPHATE KINASE from Bacillus subtilis (325 aa), FASTA scores: opt: 476, E(): 1.5e-21, (35.15% identity in 273 aa overlap); P77785|THIL_ECOLI|THIL|B0417 THIAMINE-MONOPHOSPHATE KINASE from Escherichia coli strain K12 (325 aa), FASTA scores: opt: 418, E(): 5e-18, (36.9% identity in 282 aa overlap); etc. BELONGS TO THE THIAMINE-MONOPHOSPHATE KINASE FAMILY. Note that the start, as given, is in IS1538. Protein product from Mb3001c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3001c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:A0A1R3Y4P8" /db_xref="InterPro:IPR006283" /db_xref="InterPro:IPR010918" /db_xref="InterPro:IPR016188" /db_xref="InterPro:IPR036676" /db_xref="InterPro:IPR036921" /db_xref="UniProtKB/TrEMBL:A0A1R3Y4P8" BEGIN 1 MTTKDHSLAT ESPTLQQLGE FAVIDRLVRG RRQPATVLLG PGDDAALVSA GDGRTVVSTD 61 MLVQDSHFRL DWSTPQDVGR KAIAQNAADI EAMGARATAF VVGFGAPAET PAAQASALVD 121 GMWEEAGRIG AGIVGGDLVS CRQWVVSVTA IGDLDGRAPV LRSGAKAGSV LAVVGELGRS 181 AAGYALWCNG IEDFAELRRR HLVPQPPYGH GAAAAAVGAQ AMIDVSDGLL ADLRHIAEAS 241 GVRIDLSAAA LAADRDALTA AATALGTDPW PWVLSGGEDH ALVACFVGPV PAGWRTIGRV 301 LDGPARVLVD GEEWTGYAGW QSFGEPDNQG SLG //