LOCUS SIU01622.1 553 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 Dihydroxyacetone
kinase-like protein, phosphatase domain / Dihydroxyacetone
kinase-like protein, kinase domain protein.
ACCESSION LT708304-3036
PROTEIN_ID SIU01622.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/locus_tag="BQ2027_MB2999C"
/note="Mb2999c, -, len: 553 aa. Equivalent to Rv2975c and
Rv2974c, len: 84 aa and 470 aa, from Mycobacterium
tuberculosis strain H37Rv, (100.0% identity in 83 aa
overlap and 100.0% identity in 470 aa overlap). Rv2975c:
Conserved hypothetical protein, similar to N-terminus of
others e.g. Q9ZBR4|SC7A1.09 HYPOTHETICAL 59.5 KDA PROTEIN
from Streptomyces coelicolor (589 aa), FASTA scores: opt:
141, E(): 0.0019, (41.25% identity in 80 aa overlap);
Q98R49|MYPU_1610 HYPOTHETICAL PROTEIN from Mycoplasma
pulmonis (545 aa), FASTA scores: opt: 127, E(): 0.023,
(48.0% identity in 50 aa overlap); Q9K9Z6|BH2498
HYPOTHETICAL PROTEIN from Bacillus halodurans (557 aa),
FASTA scores: opt: 126, E(): 0.028, (34.55% identity in 81
aa overlap); etc. Also some similarity with N-terminus of
P47609|Y369_MYCGE|MG369 HYPOTHETICAL PROTEIN from
Mycoplasma genitalium (557 aa), FASTA scores: opt: 108,
E(): 0.7, (36.75% identity in 49 aa overlap); this, and
preceding ORF, are similar to Y369_MYCGE and YLOV PROTEIN
but no cosmid sequence error was identified. Rv2974c:
Conserved hypothetical ala-rich protein, highly similar to
others e.g. C-terminus of Q9ZBR4|SC7A1.09 HYPOTHETICAL
59.5 KDA PROTEIN from Streptomyces coelicolor (589 aa),
FASTA scores: opt: 774, E(): 1.3e-36, (41.0% identity in
495 aa overlap); Q9K9Z6|BH2498 HYPOTHETICAL PROTEIN from
Bacillus halodurans (557 aa), FASTA scores: opt: 268, E():
8e-08, (27.7% identity in 502 aa overlap) (N-terminus
longer 76 aa); Q9X293 CONSERVED HYPOTHETICAL PROTEIN from
Thermotoga maritima (497 aa), FASTA scores: opt: 265, E():
1.1e-07, (24.9% identity in 470 aa overlap) (N-terminus
longer 43 aa); etc. Also some similarity with
P47609|Y369_MYCGE|MG369 HYPOTHETICAL PROTEIN from
Mycoplasma genitalium (557 aa), FASTA scores: opt: 154,
E(): 0.25, (20.25% identity in 489 aa overlap); this, and
following ORF, are similar to Y369_MYCGE but no cosmid
sequence error was identified.
REMARK-M.bovis-M.tuberculosis: In Mycobacterium
tuberculosis H37Rv, Rv2975c and Rv2974c exist as 2 genes.
In Mycobacterium bovis, a 2 bp deletion (cg-*) results in
a single product that is more similar to Rv2974c. Protein
product from Mb2999c detected using shotgun mass
spectrometry and SWATH mass spectrometry. Mb2999c found to
be expressed during exponential growth in Sauton's minimal
media by RNA-sequencing (TPM > 20)."
/db_xref="GOA:A0A1R3Y2Q2"
/db_xref="InterPro:IPR004007"
/db_xref="InterPro:IPR019986"
/db_xref="InterPro:IPR033470"
/db_xref="InterPro:IPR036117"
/db_xref="UniProtKB/TrEMBL:A0A1R3Y2Q2"
BEGIN
1 MGTADRPLDA SALRDWAHAV VSDLILHIDE INRLNVFPVA DSDTGVNMLF TMRAAVVEAD
61 LHANSQADAE DVARVAAALA AGALNGARGN SGVILSQILR GIAEVTATAA AASGAVLRAV
121 DANALGAALW RGVELVVASM GGVEVPGTIV SVLRAAAGAV DQCAHEGLAG AVTAAGDAAV
181 IALEKTPEQL DVLADAGAVD AGGRGLLVLL DALRSTICGQ APARAVYEPS PRALPTDTAT
241 QRPAPQFEVM YLLAVCDAAA ADQLRDRLKE LGESVAIAAA PPDSYSVHVH TDDAGAAVEA
301 GLAVGRVSRI VISALGSGTS GLPAGGWTRG RAVLAVVDGD GAAELFAGEG ACVLRPGPDA
361 VTPAADISAH QLVRAVVDTG AAHVMVLPNG YVAAEELVAG CTAAIGWGVD VVPVPTGSMV
421 QGLAALAVHD AARQAVDDGY SMARAAGASR HGSVRIATQK ALTWAGTCKP GDGLGIAGDE
481 VLIVADDVAA AAIGLVDLLL ASGGDLVTVL IGAGVTEDVA VVLERHVHDH HPGTELVSYR
541 TGHRGDALLI GVE
//