LOCUS SIU01603.1 275 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE GLYCOSYL TRANSFERASE protein. ACCESSION LT708304-3017 PROTEIN_ID SIU01603.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB2981" /note="Mb2981, -, len: 275 aa. Equivalent to Rv2957, len: 275 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 275 aa overlap). Possible glycosyl transferase (EC 2.4.1.-); possibly secreted protein. Highly similar to O88109|GSD|GTFD GSD PROTEIN from Mycobacterium avium subsp. silvaticum, Mycobacterium paratuberculosis, and Mycobacterium avium (266 aa), FASTA scores: opt: 1010, E(): 2.5e-62, (68.8% identity in 221 aa overlap). Also some similarity with other proteins and especially glycosyl transferases e.g. Q9AEE4 HYPOTHETICAL 31.4 KDA PROTEIN from Leptospira interrogans (265 aa), FASTA scores: opt: 371, E(): 3.3e-18, (34.43% identity in 212 aa overlap); Q9EXY4 PUTATIVE GLYCOSYL TRANSFERASE from Escherichia coli (248 aa), FASTA scores: opt: 339, E(): 5e-16, (32.4% identity in 210 aa overlap); Q9RCC4 GLYCOSYLTRANSFERASE-LIKE PROTEIN from Yersinia pestis (247 aa), FASTA scores: opt: 333, E(): 1.3e-15, (31.8% identity in 217 aa overlap); Q9EXY1 PUTATIVE GLYCOSYL TRANSFERASE from Escherichia coli (248 aa), FASTA scores: opt: 328, E(): 2.9e-15, (31.9% identity in 210 aa overlap); etc. Equivalent to AAK47357 from Mycobacterium tuberculosis strain CDC1551 (256 aa) but longer 19 aa. Protein product from Mb2981 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2981 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P0A5A0" /db_xref="InterPro:IPR001173" /db_xref="InterPro:IPR029044" /db_xref="UniProtKB/Swiss-Prot:P0A5A0" BEGIN 1 MVQTKRYAGL TAANTKKVAM AAPMFSIIIP TLNVAAVLPA CLDSIARQTC GDFELVLVDG 61 GSTDETLDIA NIFAPNLGER LIIHRDTDQG VYDAMNRGVD LATGTWLLFL GADDSLYEAD 121 TLARVAAFIG EHEPSDLVYG DVIMRSTNFR WGGAFDLDRL LFKRNICHQA IFYRRGLFGT 181 IGPYNLRYRV LADWDFNIRC FSNPALVTRY MHVVVASYNE FGGLSNTIVD KEFLKRLPMS 241 TRLGIRLVIV LVRRWPKVIS RAMVMRTVIS WRRRR //