LOCUS       SIU01596.1               619 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 fatty-
            acid-amp ligase fadd29 (fatty-acid-amp synthetase) (fatty-
            acid-amp synthase) protein.
ACCESSION   LT708304-3010
PROTEIN_ID  SIU01596.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="fadD29"
                     /locus_tag="BQ2027_MB2974C"
                     /note="Mb2974c, fadD29, len: 619 aa. Equivalent to
                     Rv2950c, len: 619 aa, from Mycobacterium tuberculosis
                     strain H37Rv, (99.8% identity in 619 aa overlap). Probable
                     fadD29, fatty-acid-CoA synthetase (EC 6.2.1.-), similar to
                     various mycobacterial enzymes believed to be involved in
                     polyketide or fatty acid synthesis. Equivalent (but
                     shorter 61 aa) to Q9CD84 from Mycobacterium leprae (680
                     aa), FASTA scores: opt: 3280, E(): 2.2e-192, (80.15%
                     identity in 620 aa overlap); and highly similar to others
                     from Mycobacterium leprae e.g. Q9Z5K5 PROBABLE ACYL-COA
                     SYNTHASE (583 aa), FASTA scores: opt: 2358, E(): 3.4e-136,
                     (62.35% identity in 579 aa overlap). Also similar to
                     others from Mycobacterium tuberculosis e.g.
                     Q10976|FD26_MYCTU PUTATIVE FATTY-ACID--CoA LIGASE (583
                     aa), FASTA scores: opt: 2416, E(): 1e-139, (63.15%
                     identity in 581 aa overlap) (N-terminus shorter); etc.
                     Equivalent to AAK47349 from Mycobacterium tuberculosis
                     strain CDC1551 (582 aa) but longer 37 aa. Protein product
                     from Mb2974c detected using shotgun mass spectrometry and
                     SWATH mass spectrometry. Mb2974c found to be expressed
                     during exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:Q7TXK5"
                     /db_xref="InterPro:IPR000873"
                     /db_xref="InterPro:IPR040097"
                     /db_xref="InterPro:IPR042099"
                     /db_xref="UniProtKB/Swiss-Prot:Q7TXK5"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MKTNSSFHAA GEVATQPAWG TGEQAAQPLN GSTSRFAMSE SSLADLLQKA ASQYPNRAAY
       61 KFIDYDTDPA GFTETVTWWQ VHRRAMIVAE ELWIYASSGD RVAILAPQGL EYIIAFMGVL
      121 QAGLIAVPLP VPQFGIHDER ISSALRDSAP SIILTTSSVI DEVTTYAPHA CAAQGQSAPI
      181 VVAVDALDLS SSRALDPTRF ERPSTAYLQY TSGSTRAPAG VVLSHKNVIT NCVQLMSDYI
      241 GDSEKVPSTP VSWLPFYHDM GLMLGIILPM INQDTAVLMS PMAFLQRPAR WMQLLAKHRA
      301 QISSAPNFGF ELAVRRTSDD DMAGLDLGHV RTIVTGAERV NVATLRRFTE RFAPFNLSET
      361 AIRPSYGLAE ATVYVATAGP GRAPKSVCFD YQQLSVGQAK RTENGSEGAN LVSYGAPRAS
      421 TVRIVDPETR MENPAGTVGE IWVQGDNVGL GYWRNPQQTE ATFRARLVTP SPGTSEGPWL
      481 RTGDLGVIFE GELFITGRIK ELLVVDGANH YPEDIEATIQ EITGGRVVAI AVPDDRTEKL
      541 VTIIELMKRG RTDEEEKNRL RTVKREVASA ISRSHRLRVA DVVMVAPGSI PVTTSGKVRR
      601 SASVERYLHH EFSRLDAMA
//