LOCUS SIU01596.1 619 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 fatty- acid-amp ligase fadd29 (fatty-acid-amp synthetase) (fatty- acid-amp synthase) protein. ACCESSION LT708304-3010 PROTEIN_ID SIU01596.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="fadD29" /locus_tag="BQ2027_MB2974C" /note="Mb2974c, fadD29, len: 619 aa. Equivalent to Rv2950c, len: 619 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 619 aa overlap). Probable fadD29, fatty-acid-CoA synthetase (EC 6.2.1.-), similar to various mycobacterial enzymes believed to be involved in polyketide or fatty acid synthesis. Equivalent (but shorter 61 aa) to Q9CD84 from Mycobacterium leprae (680 aa), FASTA scores: opt: 3280, E(): 2.2e-192, (80.15% identity in 620 aa overlap); and highly similar to others from Mycobacterium leprae e.g. Q9Z5K5 PROBABLE ACYL-COA SYNTHASE (583 aa), FASTA scores: opt: 2358, E(): 3.4e-136, (62.35% identity in 579 aa overlap). Also similar to others from Mycobacterium tuberculosis e.g. Q10976|FD26_MYCTU PUTATIVE FATTY-ACID--CoA LIGASE (583 aa), FASTA scores: opt: 2416, E(): 1e-139, (63.15% identity in 581 aa overlap) (N-terminus shorter); etc. Equivalent to AAK47349 from Mycobacterium tuberculosis strain CDC1551 (582 aa) but longer 37 aa. Protein product from Mb2974c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2974c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:Q7TXK5" /db_xref="InterPro:IPR000873" /db_xref="InterPro:IPR040097" /db_xref="InterPro:IPR042099" /db_xref="UniProtKB/Swiss-Prot:Q7TXK5" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MKTNSSFHAA GEVATQPAWG TGEQAAQPLN GSTSRFAMSE SSLADLLQKA ASQYPNRAAY 61 KFIDYDTDPA GFTETVTWWQ VHRRAMIVAE ELWIYASSGD RVAILAPQGL EYIIAFMGVL 121 QAGLIAVPLP VPQFGIHDER ISSALRDSAP SIILTTSSVI DEVTTYAPHA CAAQGQSAPI 181 VVAVDALDLS SSRALDPTRF ERPSTAYLQY TSGSTRAPAG VVLSHKNVIT NCVQLMSDYI 241 GDSEKVPSTP VSWLPFYHDM GLMLGIILPM INQDTAVLMS PMAFLQRPAR WMQLLAKHRA 301 QISSAPNFGF ELAVRRTSDD DMAGLDLGHV RTIVTGAERV NVATLRRFTE RFAPFNLSET 361 AIRPSYGLAE ATVYVATAGP GRAPKSVCFD YQQLSVGQAK RTENGSEGAN LVSYGAPRAS 421 TVRIVDPETR MENPAGTVGE IWVQGDNVGL GYWRNPQQTE ATFRARLVTP SPGTSEGPWL 481 RTGDLGVIFE GELFITGRIK ELLVVDGANH YPEDIEATIQ EITGGRVVAI AVPDDRTEKL 541 VTIIELMKRG RTDEEEKNRL RTVKREVASA ISRSHRLRVA DVVMVAPGSI PVTTSGKVRR 601 SASVERYLHH EFSRLDAMA //