LOCUS SIU01585.1 422 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE CONSERVED POLYKETIDE SYNTHASE ASSOCIATED PROTEIN PAPA5 protein. ACCESSION LT708304-2999 PROTEIN_ID SIU01585.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="papA5" /locus_tag="BQ2027_MB2964" /note="Mb2964, papA5, len: 422 aa. Equivalent to Rv2939, len: 422 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 422 aa overlap). Possible papA5, conserved polyketide synthase (PKS) associated protein (see first citation below), equivalent to Q49939 HYPOTHETICAL 45.6 KDA PROTEIN from Mycobacterium leprae (423 aa), FASTA scores: opt: 2398, E(): 4.5e-144, (84.05% identity in 426 aa overlap); and Q02279|YMA3_MYCBO HYPOTHETICAL 38.1 KDA PROTEIN from Mycobacterium bovis (354 aa), FASTA scores: opt: 2193, E(): 3.6e-131, (97.4% identity in 343 aa overlap). And C-terminus highly similar to to Q9S381 HYPOTHETICAL 5.0 KDA PROTEIN (FRAGMENT) from Mycobacterium leprae (44 aa), FASTA scores: opt: 275, E(): 1.4e-10, (88.65% identity in 44 aa overlap). Also similar in part to various synthetases e.g. Q9AE01|RIF20 RIF20 PROTEIN from Amycolatopsis mediterranei (Nocardia mediterranei) (403 aa), FASTA scores: opt: 282, E(): 2.7e-10, (30.3% identity in 393 aa overlap); middle part of Q00869|ESYN1 ENNIATIN SYNTHETASE (FRAGMENT) (N-methyl peptide synthetase) from Fusarium equiseti (3131 aa), FASTA scores: opt: 180, E(): 0.0036, (26.85% identity in 242 aa overlap); N-terminus of Q9FB18 PEPTIDE SYNTHETASE NRPS2-1 from Streptomyces verticillus (2626 aa), FASTA scores: opt: 159, E(): 0.068, (23.65% identity in 351 aa overlap); etc. Note that Rv2939|papA5 belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentaly). Protein product from Mb2964 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2964 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:Q02279" /db_xref="InterPro:IPR023213" /db_xref="InterPro:IPR031641" /db_xref="UniProtKB/Swiss-Prot:Q02279" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MFPGSVIRKL SHSEEVFAQY EVFTSMTIQL RGVIDVDALS DAFDALLETH PVLASHLEQS 61 SDGGWNLVAD DLLHSGICVI DGTAATNGSP SGNAELRLDQ SVSLLHLQLI LREGGAELTL 121 YLHHCMADGH HGAVLVDELF SRYTDAVTTG DPGPITPQPT PLSMEAVLAQ RGIRKQGLSG 181 AERFMSVMYA YEIPATETPA VLAHPGLPQA VPVTRLWLSK QQTSDLMAFG REHRLSLNAV 241 VAAAILLTEW QLRNTPHVPI PYVYPVDLRF VLAPPVAPTE ATNLLGAASY LAEIGPNTDI 301 VDLASDIVAT LRADLANGVI QQSGLHFGTA FEGTPPGLPP LVFCTDATSF PTMRTPPGLE 361 IEDIKGQFYC SISVPLDLYS CAVYAGQLII EHHGHIAEPG KSLEAIRSLL CTVPSEYGWI 421 ME //