LOCUS       SIU01585.1               422 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE
            CONSERVED POLYKETIDE SYNTHASE ASSOCIATED PROTEIN PAPA5
            protein.
ACCESSION   LT708304-2999
PROTEIN_ID  SIU01585.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="papA5"
                     /locus_tag="BQ2027_MB2964"
                     /note="Mb2964, papA5, len: 422 aa. Equivalent to Rv2939,
                     len: 422 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 422 aa overlap). Possible papA5,
                     conserved polyketide synthase (PKS) associated protein
                     (see first citation below), equivalent to Q49939
                     HYPOTHETICAL 45.6 KDA PROTEIN from Mycobacterium leprae
                     (423 aa), FASTA scores: opt: 2398, E(): 4.5e-144, (84.05%
                     identity in 426 aa overlap); and Q02279|YMA3_MYCBO
                     HYPOTHETICAL 38.1 KDA PROTEIN from Mycobacterium bovis
                     (354 aa), FASTA scores: opt: 2193, E(): 3.6e-131, (97.4%
                     identity in 343 aa overlap). And C-terminus highly similar
                     to to Q9S381 HYPOTHETICAL 5.0 KDA PROTEIN (FRAGMENT) from
                     Mycobacterium leprae (44 aa), FASTA scores: opt: 275, E():
                     1.4e-10, (88.65% identity in 44 aa overlap). Also similar
                     in part to various synthetases e.g. Q9AE01|RIF20 RIF20
                     PROTEIN from Amycolatopsis mediterranei (Nocardia
                     mediterranei) (403 aa), FASTA scores: opt: 282, E():
                     2.7e-10, (30.3% identity in 393 aa overlap); middle part
                     of Q00869|ESYN1 ENNIATIN SYNTHETASE (FRAGMENT) (N-methyl
                     peptide synthetase) from Fusarium equiseti (3131 aa),
                     FASTA scores: opt: 180, E(): 0.0036, (26.85% identity in
                     242 aa overlap); N-terminus of Q9FB18 PEPTIDE SYNTHETASE
                     NRPS2-1 from Streptomyces verticillus (2626 aa), FASTA
                     scores: opt: 159, E(): 0.068, (23.65% identity in 351 aa
                     overlap); etc. Note that Rv2939|papA5 belongs to the
                     transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven
                     experimentaly). Protein product from Mb2964 detected using
                     shotgun mass spectrometry and SWATH mass spectrometry.
                     Mb2964 found to be expressed during exponential growth in
                     Sauton's minimal media by RNA-sequencing."
                     /db_xref="GOA:Q02279"
                     /db_xref="InterPro:IPR023213"
                     /db_xref="InterPro:IPR031641"
                     /db_xref="UniProtKB/Swiss-Prot:Q02279"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MFPGSVIRKL SHSEEVFAQY EVFTSMTIQL RGVIDVDALS DAFDALLETH PVLASHLEQS
       61 SDGGWNLVAD DLLHSGICVI DGTAATNGSP SGNAELRLDQ SVSLLHLQLI LREGGAELTL
      121 YLHHCMADGH HGAVLVDELF SRYTDAVTTG DPGPITPQPT PLSMEAVLAQ RGIRKQGLSG
      181 AERFMSVMYA YEIPATETPA VLAHPGLPQA VPVTRLWLSK QQTSDLMAFG REHRLSLNAV
      241 VAAAILLTEW QLRNTPHVPI PYVYPVDLRF VLAPPVAPTE ATNLLGAASY LAEIGPNTDI
      301 VDLASDIVAT LRADLANGVI QQSGLHFGTA FEGTPPGLPP LVFCTDATSF PTMRTPPGLE
      361 IEDIKGQFYC SISVPLDLYS CAVYAGQLII EHHGHIAEPG KSLEAIRSLL CTVPSEYGWI
      421 ME
//