LOCUS SIU01581.1 1488 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PHENOLPTHIOCEROL
SYNTHESIS TYPE-I POLYKETIDE SYNTHASE PPSE protein.
ACCESSION LT708304-2995
PROTEIN_ID SIU01581.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/gene="ppsE"
/locus_tag="BQ2027_MB2960"
/note="Mb2960, ppsE, len: 1488 aa. Equivalent to Rv2935,
len: 1488 aa, from Mycobacterium tuberculosis strain
H37Rv, (99.9% identity in 1488 aa overlap). ppsE, type-I
polyketide synthase (see citations below), equivalent to
Q49934|PKSF|ML2353|L518_F1_8 PUTATIVE POLYKETIDE SYNTHASE
from Mycobacterium leprae (1489 aa), FASTA scores: opt:
8156, E(): 0, (82.05% identity in 1493 aa overlap). Also
similar to polyketide synthases from other bacteria e.g.
Q9RAH3|NOSB NOSB PROTEIN from Nostoc sp. GSV224 (1244 aa),
FASTA scores: opt: 2438, E(): 8.8e-137, (43.75% identity
in 969 aa overlap); Q9KIZ8|EPOC EPOC PROTEIN from
Polyangium cellulosum (1832 aa), FASTA scores: opt: 2272,
E(): 8.6e-127, (39.95% identity in 1061 aa overlap);
O54155|SC3F7.12 POLYKETIDE SYNTHASE from Streptomyces
coelicolor (2297 aa), FASTA scores: opt: 1522, E():
3.6e-82, (36.35% identity in 1057 aa overlap); etc.
Contains PS00606 Beta-ketoacyl synthases active site. Note
that Rv2935|ppsE belongs to the transcriptional unit
Rv2930|fadD26-Rv2939|papA5 (proven experimentaly). Protein
product from Mb2960 detected using shotgun mass
spectrometry and SWATH mass spectrometry. Mb2960 found to
be expressed during exponential growth in Sauton's minimal
media by RNA-sequencing."
/db_xref="GOA:Q7TXL6"
/db_xref="InterPro:IPR001227"
/db_xref="InterPro:IPR001242"
/db_xref="InterPro:IPR009081"
/db_xref="InterPro:IPR014030"
/db_xref="InterPro:IPR014031"
/db_xref="InterPro:IPR014043"
/db_xref="InterPro:IPR016035"
/db_xref="InterPro:IPR016036"
/db_xref="InterPro:IPR016039"
/db_xref="InterPro:IPR018201"
/db_xref="InterPro:IPR020801"
/db_xref="InterPro:IPR020806"
/db_xref="InterPro:IPR020841"
/db_xref="InterPro:IPR023213"
/db_xref="InterPro:IPR032821"
/db_xref="InterPro:IPR036736"
/db_xref="UniProtKB/Swiss-Prot:Q7TXL6"
/experiment="experimental evidence, no additional details
recorded"
BEGIN
1 MSIPENAIAV VGMAGRFPGA KDVSAFWSNL RRGKESIVTL SEQELRDAGV SDKTLADPAY
61 VRRAPLLDGI DEFDAGFFGF PPLAAQVLDP QHRLFLQCAW HALEDAGADP ARFDGSIGVY
121 GTSSPSGYLL HNLLSHRDPN AVLAEGLNFD QFSLFLQNDK DFLATRISHA FNLRGPSIAV
181 QTACSSSLVA VHLACLSLLS GECDMALAGG SSLCIPHRVG YFTSPGSMVS AVGHCRPFDV
241 RADGTVFGSG VGLVVLKPLA AAIDAGDRIH AVIRGSAINN DGSAKMGYAA PNPAAQADVI
301 AEAHAVSGID SSTVSYVECH GTGTPLGDPI EIQGLRAAFE VSQTSRSAPC VLGSVKSNIG
361 HLEVAAGIAG LIKTILCLKN KALPATLHYT SPNPELRLDQ SPFVVQSKYG PWECDGVRRA
421 GVSSFGVGGT NAHVVLEEAP AEASEVSAHA EPAGPQVILL SAQTAAALGE SRTALAAALE
481 TQDGPRLSDV AYTLARRRKH NVTMAAVVHD REHAATVLRA AEHDNVFVGE AAHDGEHGDR
541 ADAAPTSDRV VFLFPGQGAQ HVGMAKGLYD TEPVFAQHFD TCAAGFRDET GIDLHAEVFD
601 GTATDLERID RSQPALFTVE YALAKLVDTF GVRAGAYIGY STGEYIAATL AGVFDLQTAI
661 KTVSLRARLM HESPPGAMVA VALGPDDVTQ YLPPEVELSA VNDPGNCVVA GPKDQIRALR
721 QRLTEAGIPV RRVRATHAFH TSAMDPMLGQ FQEFLSRQQL RPPRTPLLSN LTGSWMSDQQ
781 VVDPASWTRQ ISSPIRFADE LDVVLAAPSR ILVEVGPGGS LTGSAMRHPK WSTTHRTVRL
841 MRHPLQDVDD RDTFLRALGE LWSAGVEVDW TPRRPAVPHL VSLPGYPFAR QRHWVEPNHT
901 VWAQAPGANN GSPAGTADGS TAATVDAARN GESQTEVTLQ RIWSQCLGVS SVDRNANFFD
961 LGGDSLMAIS IAMAAANEGL TITPQDLYEY PTLASLTAAV DASFASSGLA KPPEAQANPA
1021 VPPNVTYFLD RGLRDTGRCR VPLILRLDPK IGLPDIRAVL TAVVNHHDAL RLHLVGNDGI
1081 WEQHIAAPAE FTGLSNRSVP DGVAAGSPEE RAAVLGILAE LLEDQTDPNA PLAAVHIAAA
1141 HGGPHYLCLA IHAMVTDDSS RQILATDIVT AFGQRLAGEE ITLEPVSTGW REWSLRCAAL
1201 ATHPAALDTR SYWIENSTKA TLWLADALPN AHTAHPPRAD ELTKLSSTLS VEQTSELDDG
1261 RRRFRRSIQT ILLAALGRTI AQTVGEGVVA VELEGEGRSV LRPDVDLRRT VGWFTTYYPV
1321 PLACATGLGA LAQLDAVHNT LKSVPHYGIG YGLLRYVYAP TGRVLGAQRT PDIHFRYAGV
1381 IPELPSGDAP VQFDSDMTLP VREPIPGMGH AIELRVYRFG GSLHLDWWYD TRRIPAATAE
1441 ALERTFPLAL SALIQEAIAA EHTEHDDSEI VGEPEAGALV DLSSMDAG
//