LOCUS SIU01579.1 2188 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PHENOLPTHIOCEROL
SYNTHESIS TYPE-I POLYKETIDE SYNTHASE PPSC protein.
ACCESSION LT708304-2993
PROTEIN_ID SIU01579.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/gene="ppsC"
/locus_tag="BQ2027_MB2958"
/note="Mb2958, ppsC, len: 2188 aa. Equivalent to Rv2933,
len: 2188 aa, from Mycobacterium tuberculosis strain
H37Rv, (100.0% identity in 2188 aa overlap). ppsC, type-I
polyketide synthase (see citations below), highly similar
to others from Mycobacterium leprae e.g.
Q49933|PKSD|ML2355|L518_F1_3 PUTATIVE POLYKETIDE SYNTHASE
(2201 aa), FASTA scores: opt: 6973, E(): 0, (82.32%
identity in 2217 aa overlap);
Q49624|PKS3|MASA|ML1229|B1170_C2_209 PROBABLE MYCOCEROSIC
ACID SYNTHASE (2118 aa), FASTA scores: opt: 4015, E():
2.9e-208, (36.6% identity in 2184 aa overlap); etc. Also
similar to polyketide synthases from other bacteria e.g.
C-terminus of Q9L8C7 POLYKETIDE SYNTHASE from Polyangium
cellulosum (7257 aa), FASTA scores: opt: 3909, E():
3.6e-202, (40.15% identity in 2220 aa overlap);
Q9KIZ7|EPOD EPOD PROTEIN from Polyangium cellulosum (7257
aa), FASTA scores: opt: 3886, E(): 6.2e-201, (40.05%
identity in 2220 aa overlap); etc. And also highly similar
to others from Mycobacterium tuberculosis e.g.
P96291|Rv2940c (2111 aa), FASTA scores: opt: 4204, E(): 0,
(39.1% identity in 2176 aa overlap);
Q10977|PPSA_MYCTU|RV2931 PHENOLPTHIOCEROL SYNTHESIS
POLYKETIDE SYNTHASE (1876 aa), FASTA scores: opt: 3793,
E(): 2.4e-196, (46.65% identity in 1612 aa overlap); etc.
Contains PS00606 Beta-ketoacyl synthases active site, and
PS00012 Phosphopantetheine attachment site. Note that
Rv2933|ppsC belongs to the transcriptional unit
Rv2930|fadD26-Rv2939|papA5 (proven experimentaly). Protein
product from Mb2958 detected using shotgun mass
spectrometry and SWATH mass spectrometry. Mb2958 found to
be expressed during exponential growth in Sauton's minimal
media by RNA-sequencing."
/db_xref="GOA:Q7TXL8"
/db_xref="InterPro:IPR006162"
/db_xref="InterPro:IPR009081"
/db_xref="InterPro:IPR011032"
/db_xref="InterPro:IPR013154"
/db_xref="InterPro:IPR013968"
/db_xref="InterPro:IPR014030"
/db_xref="InterPro:IPR014031"
/db_xref="InterPro:IPR014043"
/db_xref="InterPro:IPR016035"
/db_xref="InterPro:IPR016036"
/db_xref="InterPro:IPR016039"
/db_xref="InterPro:IPR018201"
/db_xref="InterPro:IPR020801"
/db_xref="InterPro:IPR020806"
/db_xref="InterPro:IPR020807"
/db_xref="InterPro:IPR020841"
/db_xref="InterPro:IPR020843"
/db_xref="InterPro:IPR036291"
/db_xref="InterPro:IPR036736"
/db_xref="InterPro:IPR042104"
/db_xref="PDB:5NJI"
/db_xref="UniProtKB/Swiss-Prot:Q7TXL8"
/experiment="experimental evidence, no additional details
recorded"
BEGIN
1 MTAATPDRRA IITEALHKID DLTARLEIAE KSSSEPIAVI GMGCRFPGGV NNPEQFWDLL
61 CAGRSGIVRV PAQRWDADAY YCDDHTVPGT ICSTEGGFLT SWQPDEFDAE FFSISPREAA
121 AMDPQQRLLI EVAWEALEDA GVPQHTIRGT QTSVFVGVTA YDYMLTLAGR LRPVDLDAYI
181 PTGNSANFAA GRLAYILGAR GPAVVIDTAC SSSLVAVHLA CQSLRGRESD MALVGGTNLL
241 LSPGPSIACS RWGMLSPEGR CKTFDASADG YVRGEGAAVV VLKRLDDAVR DGNRILAVVR
301 GSAVNQDGAS SGVTVPNGPA QQALLAKALT SSKLTAADID YVEAHGTGTP LGDPIELDSL
361 SKVFSDRAGS DQLVIGSVKT NLGHLEAAAG VAGLMKAVLA VHNGYIPRHL NFHQLTPHAS
421 EAASRLRIAA DGIDWPTTGR PRRAGVSSFG VSGTNAHVVI EQAPDPMAAA GTEPQRGPVP
481 AVSTLVVFGK TAPRVAATAS VLADWLDGPG AAVPLADVAH TLNHHRARQT RFGTVAAVDR
541 RQAVIGLRAL AAGQSAPGVV APREGSIGGG TVFVYSGRGS QWAGMGRQLL ADEPAFAAAI
601 AELEPEFVAQ GGFSLRDVIA GGKELVGIEQ IQLGLIGMQL ALTALWRSYG VTPDAVIGHS
661 MGEVAAAVVA GALTPAQGLR VTAVRSRLMA PLSGQGTMAL LELDAEATEA LIADYPEVSL
721 GIYASPRQTV ISGPPLLIDE LIDKVRQQNG FATRVNIEVA PHNPAMDALQ PAMRSELADL
781 TPQPPTIPII STTYADLGIS LGSGPRFDAE HWATNMRNPV RFHQAIAHAG ADHHTFIEIS
841 AHPLLTHSIS DTLRASYDVD NYLSIGTLQR DAHDTLEFHT NLNTTHTTHP PQTPHPPEPH
901 PVLPTTPWQH TQHWITATSA AYHRPDTHPL LGVGVTDPTN GTRVWESELD PDLLWLADHV
961 IDDLVVLPGA AYAEIALAAA TDTFAVEQDQ PWMISELDLR QMLHVTPGTV LVTTLTGDEQ
1021 RCQVEIRTRS GSSGWTTHAT ATVARAEPLA PLDHEGQRRE VTTADLEDQL DPDDLYQRLR
1081 GAGQQHGPAF QGIVGLAVTQ AGVARAQVRL PASARTGSRE FMLHPVMMDI ALQTLGATRT
1141 ATDLAGGQDA RQGPSSNSAL VVPVRFAGVH VYGDITRGVR AVGSLAAAGD RLVGEVVLTD
1201 ANGQPLLVVD EVEMAVLGSG SGATELTNRL FMLEWEPAPL EKTAEATGAL LLIGDPAAGD
1261 PLLPALQSSL RDRITDLELA SAADEATLRA AISRTSWDGI VVVCPPRAND ESMPDEAQLE
1321 LARTRTLLVA SVVETVTRMG ARKSPRLWIV TRGAAQFDAG ESVTLAQTGL RGIARVLTFE
1381 HSELNTTLVD IEPDGTGSLA ALAEELLAGS EADEVALRDG QRYVNRLVPA PTTTSGDLAA
1441 EARHQVVNLD SSGASRAAVR LQIDQPGRLD ALNVHEVKRG RPQGDQVEVR VVAAGLNFSD
1501 VLKAMGVYPG LDGAAPVIGG ECVGYVTAIG DEVDGVEVGQ RVIAFGPGTF GTHLGTIADL
1561 VVPIPDTLAD NEAATFGVAY LTAWHSLCEV GRLSPGERVL IHSATGGVGM AAVSIAKMIG
1621 ARIYTTAGSD AKREMLSRLG VEYVGDSRSV DFADEILELT DGYGVDVVLN SLAGEAIQRG
1681 VQILAPGGRF IELGKKDVYA DASLGLAALA KSASFSVVDL DLNLKLQPAR YRQLLQHILQ
1741 HVADGKLEVL PVTAFSLHDA ADAFRLMASG KHTGKIVISI PQHGSIEAIA APPPLPLVSR
1801 DGGYLIVGGM GGLGFVVARW LAEQGAGLIV LNGRSAPSDE VAAAIAELNA SGSRIEVITG
1861 DITEPDTAER LVRAVEDAGF RLAGVVHSAM VLADEIVLNM TDSAARRVFA PKVTGSWRLH
1921 VATAARDVDW WLTFSSAAAL LGTPGQGAYA AANSWVDGLV AHRRSAGLPA VGINWGPWAD
1981 VGRAQFFKDL GVEMINAEQG LAAMQAVLTA DRGRTGVFSL DARQWFQSFP AVAGSSLFAK
2041 LHDSAARKSG QRRGGGAIRA QLDALDAAER PGHLASAIAD EIRAVLRSGD PIDHHRPLET
2101 LGLDSLMGLE LRNRLEASLG ITLPVALVWA YPTISDLATA LCERMDYATP AAAQEISDTE
2161 PELSDEEMDL LADLVDASEL EAATRGES
//