LOCUS SIU01577.1 1876 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PHENOLPTHIOCEROL
SYNTHESIS TYPE-I POLYKETIDE SYNTHASE PPSA protein.
ACCESSION LT708304-2991
PROTEIN_ID SIU01577.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/gene="ppsA"
/locus_tag="BQ2027_MB2956"
/note="Mb2956, ppsA, len: 1876 aa. Equivalent to Rv2931,
len: 1876 aa, from Mycobacterium tuberculosis strain
H37Rv, (99.6% identity in 1876 aa overlap). ppsA, type-I
polyketide synthase (see citations below), highly similar
to others from Mycobacterium leprae e.g.
Q9Z5K6|ML2357|MLCB12.02c PUTATIVE POLYKETIDE SYNTHASE from
Mycobacterium leprae (1871 aa), FASTA scores: opt: 7566,
E(): 0, (76.1% identity in 1888 aa overlap);
Q9S384|ML2356|MLCB12.01c PUTATIVE POLYKETIDE SYNTHASE from
Mycobacterium leprae (1540 aa), FASTA scores: opt: 4026,
E(): 9.8e-212, (45.7% identity in 1811 aa overlap);
Q49932|PKSC|L518_F1_2 PUTATIVE POLYKETIDE SYNTHASE (1446
aa), FASTA scores: opt: 4026, E(): 9.4e-212, (70.6%
identity in 885 aa overlap). Also similar to polyketide
synthases from other bacteria e.g. C-terminus of
Q9L8C7|EPOC POLYKETIDE SYNTHASE from Polyangium cellulosum
(7257 aa), FASTA scores: opt: 2592, E(): 5.2e-133, (32.55%
identity in 2245 aa overlap); P22367|MSAS_PENPA
6-methylsalicylic acid synthase from Penicillium patulum
(Penicillium griseofulvum) (1774 aa), FASTA scores: opt:
2391, E(): 0, (34.2% identity in 1815 aa overlap); etc.
And also highly similar to others from Mycobacterium
tuberculosis e.g. Q10978|PPSB_MYCTU|RV2932
PHENOLPTHIOCEROL SYNTHESIS POLYKETIDE SYNTHASE (1538 aa),
FASTA scores: opt: 4227, E(): 0, (46.8% identity in 1810
aa overlap) (gap in middle); etc. Contains PS00606
Beta-ketoacyl synthases active site, and PS00012
Phosphopantetheine attachment site. Note that Rv2931|ppsA
belongs to the transcriptional unit
Rv2930|fadD26-Rv2939|papA5 (proven experimentaly). Protein
product from Mb2956 detected using shotgun mass
spectrometry and SWATH mass spectrometry. Mb2956 found to
be expressed during exponential growth in Sauton's minimal
media by RNA-sequencing."
/db_xref="GOA:Q7TXM0"
/db_xref="InterPro:IPR006162"
/db_xref="InterPro:IPR009081"
/db_xref="InterPro:IPR013968"
/db_xref="InterPro:IPR014030"
/db_xref="InterPro:IPR014031"
/db_xref="InterPro:IPR014043"
/db_xref="InterPro:IPR016035"
/db_xref="InterPro:IPR016036"
/db_xref="InterPro:IPR016039"
/db_xref="InterPro:IPR018201"
/db_xref="InterPro:IPR020801"
/db_xref="InterPro:IPR020806"
/db_xref="InterPro:IPR020807"
/db_xref="InterPro:IPR020841"
/db_xref="InterPro:IPR032821"
/db_xref="InterPro:IPR036291"
/db_xref="InterPro:IPR036736"
/db_xref="InterPro:IPR042104"
/db_xref="UniProtKB/Swiss-Prot:Q7TXM0"
/experiment="experimental evidence, no additional details
recorded"
BEGIN
1 MTGSISGEAD LRHWLIDYLV TNIGCTPDEV DPDLSLADLG VSSRDAVVLS GELSELLGRT
61 VSPIDFWEHP TINALAAYLA APEPSPDSDA AVKRGARNSL DEPIAVVGMG CRFPGGISCP
121 EALWDFLCER RSSISQVPPQ RWQPFEGGPP EVAAALARTT RWGSFLPDID AFDAEFFEIS
181 PSEADKMDPQ QRLLLEVAWE ALEHAGIPPG TLRRSATGVF AGACLSEYGA MASADLSQVD
241 GWSNSGGAMS IIANRLSYFL DLRGPSVAVD TACSSSLVAI HLACQSLRTQ DCHLAIAAGV
301 NLLLSPAVFR GFDQVGALSP TGQCRAFDAT ADGFVRGEGA GVVVLKRLTD AQRDGDRVLA
361 VICGSAVTQD GRSNGLMAPN PAAQMAVLRA AYTNAGMQPS EVDYVEAHGT GTLLGDPIEA
421 RALGTVLGRG RPEDSPLLIG SVKTNLGHTE AAAGIAGFIK TVLAVQHGQI PPNQHFETAN
481 PHIPFTDLRM KVVDTQTEWP ATGHPRRAGV SSFGFGGTNA HVVIEQGQEV RPAPGQGLSP
541 AVSTLVVAGK TMQRVSATAG MLADWMEGPG ADVALADVAH TLNHHRSRQP KFGTVVARDR
601 TQAIAGLRAL AAGQHAPGVV NPAEGSPGPG TVFVYSGRGS QWAGMGRQLL ADEPAFAAAV
661 AELEPVFVEQ AGFSLHDVLA NGEELVGIEQ IQLGLIGMQL ALTELWCSYG VQPDLVIGHS
721 MGEVAAAVVA GALTPAEGLR VTATRSRLMA PLSGQGGMAL LELDAPTTEA LIADFPQVTL
781 GIYNSPRQTV IAGPTEQIDE LITRVRARDR FASRVNIEVA PHNPAMDALQ PAMRSELADL
841 TPRTPTIGII STTYADLHTQ PVFDAEHWAT NMRNPVHFQQ AIASAGSGAD GAYHTFIEIS
901 AHPLLTQAII DTLHSAQPGA RYTSLGTLQR DTDDVVTFRT NLNKAHTIHP PHTPHPPEPH
961 PPIPTTPWQH TRHWITTKYP AGSVGSAPRA GTLLGQHTTV ATVSASPPSH LWQARLAPDA
1021 KPYQGGHRFH QVEVVPASVV LHTILSAATE LGYSALSEVR FEQPIFADRP RLIQVVADNR
1081 AISLASSPAA GTPSDRWTRH VTAQLSSSPS DSASSLNEHH RANGQPPERA HRDLIPDLAE
1141 LLAMRGIDGL PFSWTVASWT QHSSNLTVAI DLPEALPEGS TGPLLDAAVH LAALSDVADS
1201 RLYVPASIEQ ISLGDVVTGP RSSVTLNRTA HDDDGITVDV TVAAHGEVPS LSMRSLRYRA
1261 LDFGLDVGRA QPPASTGPVE AYCDATNFVH TIDWQPQTVP DATHPGAEQV THPGPVAIIG
1321 DDGAALCETL EGAGYQPAVM SDGVSQARYV VYVADSDPAG ADETDVDFAV RICTEITGLV
1381 RTLAERDADK PAALWILTRG VHESVAPSAL RQSFLWGLAG VIAAEHPELW GGLVDLAIND
1441 DLGEFGPALA ELLAKPSKSI LVRRDGVVLA PALAPVRGEP ARKSLQCRPD AAYLITGGLG
1501 ALGLLMADWL ADRGAHRLVL TGRTPLPPRR DWQLDTLDTE LRRRIDAIRA LEMRGVTVEA
1561 VAADVGCRED VQALLAARDR DGAAPIRGII HAAGITNDQL VTSMTGDAVR QVMWPKIGGS
1621 QVLHDAFPPG SVDFFYLTAS AAGIFGIPGQ GSYAAANSYL DALARARRQQ GCHTMSLDWV
1681 AWRGLGLAAD AQLVSEELAR MGSRDITPSE AFTAWEFVDG YDVAQAVVVP MPAPAGADGS
1741 GANAYLLPAR NWSVMAATEV RSELEQGLRR IIAAELRVPE KELDTDRPFA ELGLNSLMAM
1801 AIRREAEQFV GIELSATMLF NHPTVKSLAS YLAKRVAPHD VSQDNQISAL SSSAGSVLDS
1861 LFDRIESAPP EAERSV
//