LOCUS SIU01570.1 289 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE FORMAMIDOPYRIMIDINE-DNA GLYCOSYLASE FPG (FAPY-DNA GLYCOSYLASE) protein. ACCESSION LT708304-2984 PROTEIN_ID SIU01570.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="fpg" /locus_tag="BQ2027_MB2949C" /note="Mb2949c, fpg, len: 289 aa. Equivalent to Rv2924c, len: 289 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 289 aa overlap). Probable fpg (alternate gene name: mutM), formamidopyrimidine-DNA glycosylase (EC 3.2.2.23), equivalent to O69470|FPG_MYCLE FORMAMIDOPYRIMIDINE-DNA GLYCOSYLASE from Mycobacterium leprae (282 aa), FASTA scores: opt: 1563, E(): 1.3e-96, (80.6% identity in 289 aa overlap). Also highly similar to other formamidopyrimidine-DNA glycosylases e.g. Q9ZBQ6|FPG_STRCO from Streptomyces coelicolor (286 aa), FASTA scores: opt: 1047, E(): 2.9e-62, (57.55% identity in 292 aa overlap); P95744|FPG_SYNEN from Synechococcus elongatus naegeli (284 aa), FASTA scores: opt: 569, E(): 1.9e-30, (37.95% identity in 290 aa overlap); P05523|FPG_ECOLI|MUTM|FPG|B3635 from Escherichia coli strain K12 (269 aa), FASTA scores: opt: 424, E(): 8.2e-21, (33.9% identity in 289 aa overlap); etc. BELONGS TO THE FPG FAMILY. COFACTOR: BINDS 1 ZINC ION. Mb2949c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P64151" /db_xref="InterPro:IPR000214" /db_xref="InterPro:IPR010663" /db_xref="InterPro:IPR010979" /db_xref="InterPro:IPR012319" /db_xref="InterPro:IPR015886" /db_xref="InterPro:IPR015887" /db_xref="InterPro:IPR020629" /db_xref="InterPro:IPR035937" /db_xref="UniProtKB/Swiss-Prot:P64151" BEGIN 1 MPELPEVEVV RRGLQAHVTG RTITEVRVHH PRAVRRHDAG PADLTARLRG ARINGTDRRG 61 KYLWLTLNTA GVHRPTDTAL VVHLGMSGQM LLGAVPCAAH VRISALLDDG TVLSFADQRT 121 FGGWLLADLV TVDGSVVPVP VAHLARDPLD PRFDCDAVVK VLRRKHSELK RQLLDQRVVS 181 GIGNIYADEA LWRAKVNGAH VAATLRCRRL GAVLHAAADV MREALAKGGT SFDSLYVNVN 241 GESGYFERSL DAYGREGENC RRCGAVIRRE RFMNRSSFYC PRCQPRPRK //