LOCUS       SIU01525.1               364 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 CONSERVED
            HYPOTHETICAL PROTEIN protein.
ACCESSION   LT708304-2939
PROTEIN_ID  SIU01525.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /locus_tag="BQ2027_MB2904C"
                     /note="Mb2904c, -, len: 364 aa. Equivalent to 5' end of
                     Rv2880c and 3' end of Rv2879c, len: 275 aa and 189 aa,
                     from Mycobacterium tuberculosis strain H37Rv, (100.0%
                     identity in 177 aa overlap and 100.0% identity in 188 aa
                     overlap). Rv2880c: Conserved hypothetical protein, highly
                     similar in N-terminus to others e.g. O86754|SC6A9.22c
                     HYPOTHETICAL 40.4 KDA PROTEIN from Streptomyces coelicolor
                     (368 aa), FASTA scores: opt: 663, E(): 2.6e-33, (52.6%
                     identity in 213 aa overlap); Q55880|Y098_SYNY3|SLL0098
                     HYPOTHETICAL 38.9 KDA PROTEIN from Synechocystis sp.
                     strain PCC 6803 (350 aa), FASTA scores: opt: 362, E():
                     7.3e-15, (38.9% identity in 162 aa overlap); O66732|AQ_416
                     HYPOTHETICAL 40.2 KDA PROTEIN from Aquifex aeolicus (348
                     aa), FASTA scores: opt: 321, E(): 2.4e-12, (39.75%
                     identity in 146 aa overlap); etc. Appears to be a frame
                     shift with respect to preceding ORF but we can detect no
                     error in the cosmid sequence to account for this. Rv2879c:
                     Conserved hypothetical protein, similar to others e.g.
                     C-terminus of Q9RVT6|DR0936 CONSERVED HYPOTHETICAL PROTEIN
                     from Deinococcus radiodurans (346 aa), FASTA scores: opt:
                     505, E(): 1e-26, (46.5% identity in 185 aa overlap);
                     O34617|YLON_BACSU HYPOTHETICAL 41.6 KDA PROTEIN from
                     Bacillus subtilis (363 aa), FASTA scores: opt: 459, E():
                     1.2e-24, (40.5% identity in 185 aa overlap);
                     YFGB_ECOLI|P36979 hypothetical 43.1 kd protein from
                     Escherichia coli (384 aa), FASTA scores, opt: 410, E():
                     2.8e-21, (41.7% identity in 187 aa overlap); etc. Appears
                     to be a frame shift with respect to following ORF but we
                     can detect no error in the cosmid sequence to account for
                     this. REMARK-M.bovis-M.tuberculosis: In Mycobacterium
                     tuberculosis strain H37Rv, Rv2880c and Rv2879c exist as 2
                     genes. In Mycobacterium bovis, a single base deletion
                     (g-*) leads to a single product. Protein product from
                     Mb2904c detected using SWATH mass spectrometry and 0.
                     Mb2904c found to be expressed during exponential growth in
                     Sauton's minimal media by RNA-sequencing (TPM > 20)."
                     /db_xref="GOA:P0A645"
                     /db_xref="InterPro:IPR004383"
                     /db_xref="InterPro:IPR007197"
                     /db_xref="InterPro:IPR013785"
                     /db_xref="InterPro:IPR027492"
                     /db_xref="InterPro:IPR040072"
                     /db_xref="UniProtKB/Swiss-Prot:P0A645"
BEGIN
        1 MVPELMFDEP RPGRPPRHLA DLDAAGRASA VAELGLPAFR AKQLAHQYYG RLIADPRQMT
       61 DLPAAVRDRI AGAMFPNLLT ASADITCDAG QTRKTLWRAV DGTMFESVLM RYPRRNTVCI
      121 SSQAGCGMAC PFCATGQGGL TRNLSTAEIL EQVRAGAAAL RDDFGDRLSN VVFMGMGEPL
      181 ANYARVLAAV QRITARPPSG FGISARAVTV STVGLAPAIR NLADARLGVT LALSLHAPDD
      241 GLRDTLVPVN NRWRISEALD AARYYANVTG RRVSIEYALI RDVNDQPWRA DLLGKRLHRV
      301 LGPLAHVNLI PLNPTPGSDW DASPKPVERE FVKRVRAKGV SCTVRDTRGR EISAACGQLA
      361 AVGG
//