LOCUS SIU01524.1 173 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 soluble secreted antigen mpt53 precursor protein. ACCESSION LT708304-2938 PROTEIN_ID SIU01524.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="mpt53" /locus_tag="BQ2027_MB2903C" /standard_name="mpt53" /note="Mb2903c, mpb53, len: 173 aa. Equivalent to Rv2878c, len: 173 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 173 aa overlap). mpt53, secreted protein (contains N-terminal signal sequence) (see citations below). Shows some similarity with several disulfide bond interchange proteins e.g. P43787|THIX_HAEIN THIOREDOXIN-LIKE PROTEIN HI1115 from Haemophilus influenzae (167 aa), FASTA scores: opt: 200, E(): 1.4e-06, (28.9% identity in 135 aa overlap); P52237|TIPB_PSEFL THIOL:DISULFIDE INTERCHANGE PROTEIN TIPB PRECURSOR (CYTOCHROME C BIOGENESIS PROTEIN TIPB) (178 aa), FASTA scores: opt: 184, E(): 1.8e-05, (26.3% identity in 171 aa overlap); etc. Also highly similar to O53924|DSBF|Rv1677|MTV047.12 PUTATIVE LIPOPROTEIN from Mycobacterium tuberculosis (182 aa), FASTA scores: opt: 482, E(): 5.7e-26, (52.8% identity in 142 aa overlap). COULD BE BELONG TO THE THIOREDOXIN FAMILY. Note that also previously known as dsbE. Protein product from Mb2903c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2903c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P0A619" /db_xref="InterPro:IPR000866" /db_xref="InterPro:IPR013766" /db_xref="InterPro:IPR036249" /db_xref="UniProtKB/Swiss-Prot:P0A619" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MSLRLVSPIK AFADGIVAVA IAVVLMFGLA NTPRAVAADE RLQFTATTLS GAPFDGASLQ 61 GKPAVLWFWT PWCPFCNAEA PSLSQVAAAN PAVTFVGIAT RADVGAMQSF VSKYNLNFTN 121 LNDADGVIWA RYNVPWQPAF VFYRADGTST FVNNPTAAMS QDELSGRVAA LTS //