LOCUS SIU01504.1 308 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE AMIDOTRANSFERASE protein. ACCESSION LT708304-2918 PROTEIN_ID SIU01504.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB2884C" /note="Mb2884c, -, len: 308 aa. Equivalent to Rv2859c, len: 308 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 308 aa overlap). Possible amidotransferase (EC 6.3.5.- or 2.-.-.-), equivalent (but longer 58 aa) to Q9CBU9|ML1573 POSSIBLE AMIDOTRANSFERASE from Mycobacterium leprae (249 aa), FASTA scores: opt: 1226, E(): 3e-64, (71.55% identity in 239 aa overlap). Also similar to other amidotransferases and hypothetical proteins, but shorter in N-terminus e.g. O88072|SCI35.37 HYPOTHETICAL 25.3 KDA PROTEIN from Streptomyces coelicolor (242 aa), FASTA scores: opt: 683, E(): 1.2e-32, (47.65% identity in 235 aa overlap); AAK79730|Q97I88|CAC1764 PREDICTED GLUTAMINE AMIDOTRANSFERASE from Clostridium acetobutylicum (241 aa), FASTA scores: opt: 458, E(): 1.6e-19, (32.95% identity in 246 aa overlap); AAK75201|Q97QV9|SP1089 GLUTAMINE AMIDOTRANSFERASE CLASS I from Streptococcus pneumoniae (229 aa), FASTA scores: opt: 431, E(): 5.6e-18, (34.75% identity in 236 aa overlap); etc. Contains three 17 aa repeats at the N-terminus very similar to those in other Mycobacterium tuberculosis proteins e.g. Q10699|YY30_MYCTU|Rv2090|MT2151|MTCY49.30 PUTATIVE 5'-3' EXONUCLEASE RV2090 (EC 3.1.11.-). Protein product from Mb2884c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2884c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:A0A1R3Y2F8" /db_xref="InterPro:IPR011697" /db_xref="InterPro:IPR017926" /db_xref="InterPro:IPR029062" /db_xref="UniProtKB/TrEMBL:A0A1R3Y2F8" BEGIN 1 MDLSASRSDG GDPLRPASPR LRSPVSDGGD PLRPASPRLR SPVSDGGDPL RPASPRLRSP 61 LGASRPVVGL TAYLEQVRTG VWDIPAGYLP ADYFEGITMA GGVAVLLPPQ PVDPESVGCV 121 LDSLHALVIT GGYDLDPAAY GQEPHPATDH PRPGRDAWEF ALLRGALQRG MPVLGICRGT 181 QVLNVALGGT LHQHLPDILG HSGHRAGNGV FTRLPVHTAS GTRLAELIGE SADVPCYHHQ 241 AIDQVGEGLV VSAVDVDGVI EALELPGDTF VLAVQWHPEK SLDDLRLFKA LVDAASGYAG 301 RQSQAEPR //