LOCUS       SIU01492.1               405 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE
            MULTIFUNCTIONAL ENZYME SIROHEME SYNTHASE CYSG: UROPORPHYRIN-
            III C-METHYLTRANSFERASE (UROGEN III METHYLASE) (SUMT)
            (UROPORPHYRINOGEN III METHYLASE) (UROM) + PRECORRIN-2
            OXIDASE + FERROCHELATASE protein.
ACCESSION   LT708304-2906
PROTEIN_ID  SIU01492.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="cysG"
                     /locus_tag="BQ2027_MB2872C"
                     /note="Mb2872c, cysG, len: 405 aa. Equivalent to Rv2847c,
                     len: 405 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 405 aa overlap). Possible cysG,
                     multifunctional enzyme, siroheme synthase containing
                     uroporphyrin-iii c-methyltransferase (EC 2.1.1.107),
                     precorrin-2 oxidase (EC 1.-.-.-) and ferrochelatase (EC
                     4.99.1.-). C-terminus highly similar to many
                     uroporphyrin-iii c-methyltransferases e.g. Q51720|COBA
                     UROPORPHYRINOGEN III METHYLTRANSFERASE from
                     Propionibacterium freudenreichii (257 aa), FASTA scores:
                     opt: 776, E(): 1.5e-39, (48.95% identity in 243 aa
                     overlap); Q9HMY4|UROM|VNG2331G
                     S-ADENOSYL-L-METHIONINE:UROPORPHYRINOGEN III
                     METHYLTRANSFERASE from Halobacterium sp. strain NRC-1 (246
                     aa), FASTA scores: opt: 704, E(): 3.1e-35, (49.4% identity
                     in 245 aa overlap); P42437|NASF_BACSU|NASBE
                     UROPORPHYRIN-III C-METHYLTRANSFERASE from Bacillus
                     subtilis (483 aa), FASTA scores: opt: 610, E(): 2.4e-29,
                     (42.1% identity in 240 aa overlap); etc. And highly
                     similar over entire length to other proteins e.g.
                     Q9L1C9|SCL11.09c UROPORPHYRINOGEN III METHYLTRANSFERASE
                     from Streptomyces coelicolor (410 aa), FASTA scores: opt:
                     1481, E(): 5.6e-82, (58.45% identity in 409 aa overlap);
                     Q9I0M7|CYSG|PA2611 SIROHEME SYNTHASE from Pseudomonas
                     aeruginosa (465 aa), FASTA scores: opt: 609, E(): 2.7e-29,
                     (34.7% identity in 444 aa overlap);
                     P11098|CYSG_ECOLI|B3368|Z4729|ECS4219 SIROHEME SYNTHASE
                     from Escherichia coli stains O157:H7 and K12 (457 aa),
                     FASTA scores: opt: 543, E(): 9.1e-27, (31.3% identity in
                     450 aa overlap); etc. BELONGS TO A FAMILY THAT GROUPS
                     SUMT, CYSG, CBIF/COBM AND CBIL/COBI. Note that previously
                     known as cysG2. Protein product from Mb2872c detected
                     using shotgun mass spectrometry and SWATH mass
                     spectrometry. Mb2872c found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:A0A1R3Y2D0"
                     /db_xref="InterPro:IPR000878"
                     /db_xref="InterPro:IPR006366"
                     /db_xref="InterPro:IPR012409"
                     /db_xref="InterPro:IPR014776"
                     /db_xref="InterPro:IPR014777"
                     /db_xref="InterPro:IPR035996"
                     /db_xref="InterPro:IPR036291"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3Y2D0"
BEGIN
        1 MTENPYLVGL RLAGKKVVVV GGGTVAQRRL PLLIASGADV HVIAPSVTPA VEAMDQITLS
       61 VRDYRDGDLD GAWYAIAATD DARVNVAVVA EAERRRIFCV RADIAVEGTA VTPASFSYAG
      121 LSVGVLAGGE HRRSAAIRSA IREALQQGVI TAQSSDVLSG GVALVGGGPG DPELITVRGR
      181 RLLAQADVVV ADRLAPPELL AELPPHVEVI DAAKIPYGRA MAQDAINAVL IERARSGNFV
      241 VRLKGGDPFV FARGYEEVLA CAHAGIPVTV VPGVTSAIAV PAMAGVPVTH RAMTHEFVVV
      301 SGHLAPGHPE SLVNWDALAA LTGTIVLLMA VERIELFVDV LLKGGRTADT PVLVVQHGTT
      361 AAQQTLRATL ADTPEKVRAA GIRPPAIIVI GAVVGLSGVR GLNNS
//