LOCUS SIU01492.1 405 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE MULTIFUNCTIONAL ENZYME SIROHEME SYNTHASE CYSG: UROPORPHYRIN- III C-METHYLTRANSFERASE (UROGEN III METHYLASE) (SUMT) (UROPORPHYRINOGEN III METHYLASE) (UROM) + PRECORRIN-2 OXIDASE + FERROCHELATASE protein. ACCESSION LT708304-2906 PROTEIN_ID SIU01492.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="cysG" /locus_tag="BQ2027_MB2872C" /note="Mb2872c, cysG, len: 405 aa. Equivalent to Rv2847c, len: 405 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 405 aa overlap). Possible cysG, multifunctional enzyme, siroheme synthase containing uroporphyrin-iii c-methyltransferase (EC 2.1.1.107), precorrin-2 oxidase (EC 1.-.-.-) and ferrochelatase (EC 4.99.1.-). C-terminus highly similar to many uroporphyrin-iii c-methyltransferases e.g. Q51720|COBA UROPORPHYRINOGEN III METHYLTRANSFERASE from Propionibacterium freudenreichii (257 aa), FASTA scores: opt: 776, E(): 1.5e-39, (48.95% identity in 243 aa overlap); Q9HMY4|UROM|VNG2331G S-ADENOSYL-L-METHIONINE:UROPORPHYRINOGEN III METHYLTRANSFERASE from Halobacterium sp. strain NRC-1 (246 aa), FASTA scores: opt: 704, E(): 3.1e-35, (49.4% identity in 245 aa overlap); P42437|NASF_BACSU|NASBE UROPORPHYRIN-III C-METHYLTRANSFERASE from Bacillus subtilis (483 aa), FASTA scores: opt: 610, E(): 2.4e-29, (42.1% identity in 240 aa overlap); etc. And highly similar over entire length to other proteins e.g. Q9L1C9|SCL11.09c UROPORPHYRINOGEN III METHYLTRANSFERASE from Streptomyces coelicolor (410 aa), FASTA scores: opt: 1481, E(): 5.6e-82, (58.45% identity in 409 aa overlap); Q9I0M7|CYSG|PA2611 SIROHEME SYNTHASE from Pseudomonas aeruginosa (465 aa), FASTA scores: opt: 609, E(): 2.7e-29, (34.7% identity in 444 aa overlap); P11098|CYSG_ECOLI|B3368|Z4729|ECS4219 SIROHEME SYNTHASE from Escherichia coli stains O157:H7 and K12 (457 aa), FASTA scores: opt: 543, E(): 9.1e-27, (31.3% identity in 450 aa overlap); etc. BELONGS TO A FAMILY THAT GROUPS SUMT, CYSG, CBIF/COBM AND CBIL/COBI. Note that previously known as cysG2. Protein product from Mb2872c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2872c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y2D0" /db_xref="InterPro:IPR000878" /db_xref="InterPro:IPR006366" /db_xref="InterPro:IPR012409" /db_xref="InterPro:IPR014776" /db_xref="InterPro:IPR014777" /db_xref="InterPro:IPR035996" /db_xref="InterPro:IPR036291" /db_xref="UniProtKB/TrEMBL:A0A1R3Y2D0" BEGIN 1 MTENPYLVGL RLAGKKVVVV GGGTVAQRRL PLLIASGADV HVIAPSVTPA VEAMDQITLS 61 VRDYRDGDLD GAWYAIAATD DARVNVAVVA EAERRRIFCV RADIAVEGTA VTPASFSYAG 121 LSVGVLAGGE HRRSAAIRSA IREALQQGVI TAQSSDVLSG GVALVGGGPG DPELITVRGR 181 RLLAQADVVV ADRLAPPELL AELPPHVEVI DAAKIPYGRA MAQDAINAVL IERARSGNFV 241 VRLKGGDPFV FARGYEEVLA CAHAGIPVTV VPGVTSAIAV PAMAGVPVTH RAMTHEFVVV 301 SGHLAPGHPE SLVNWDALAA LTGTIVLLMA VERIELFVDV LLKGGRTADT PVLVVQHGTT 361 AAQQTLRATL ADTPEKVRAA GIRPPAIIVI GAVVGLSGVR GLNNS //