LOCUS SIU01484.1 900 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE TRANSLATION INITIATION FACTOR IF-2 INFB protein. ACCESSION LT708304-2898 PROTEIN_ID SIU01484.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="infB" /locus_tag="BQ2027_MB2864C" /note="Mb2864c, infB, len: 900 aa. Equivalent to Rv2839c, len: 900 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 900 aa overlap). Probable infB, translation initiation factor IF-2, highly similar, but in part, to Q9Z5I9|IF2_MYCLE|ML1556|MLCB596.14 TRANSLATION INITIATION FACTOR IF-2 from Mycobacterium leprae (924 aa), FASTA scores: opt: 4548, E(): 2.4e-132, (83.6% identity in 933 aa overlap). Also similar in part to others e.g. Q9K3E2|SC5H4.30 from Streptomyces coelicolor (835 aa), FASTA scores: opt: 2559, E(): 1.3e-71, (59.9% identity in 833 aa overlap); P17889|IF2_BACSU|INFB from Bacillus subtilis (716 aa), FASTA scores: opt: 1782, E(): 6.6e-48, (46.65% identity in 686 aa overlap); P02995|IF2_ECOLI|INFB|SSYG|B3168|Z4529|ECS4049 from Escherichia coli strains O157:H7 and K12 (890 aa), FASTA scores: opt: 1708, E(): 1.3e-45, (46.2% identity in 662 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). BELONGS TO THE IF-2 FAMILY. Protein product from Mb2864c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2864c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P65132" /db_xref="InterPro:IPR000178" /db_xref="InterPro:IPR000795" /db_xref="InterPro:IPR005225" /db_xref="InterPro:IPR006847" /db_xref="InterPro:IPR009000" /db_xref="InterPro:IPR015760" /db_xref="InterPro:IPR023115" /db_xref="InterPro:IPR027417" /db_xref="InterPro:IPR036925" /db_xref="UniProtKB/Swiss-Prot:P65132" BEGIN 1 MAAGKARVHE LAKELGVTSK EVLARLSEQG EFVKSASSTV EAPVARRLRE SFGGSKPAPA 61 KGTAKSPGKG PDKSLDKALD AAIDMAAGNG KATAAPAKAA DSGGAAIVSP TTPAAPEPPT 121 AVPPSPQAPH PGMAPGARPG PVPKPGIRTP RVGNNPFSSA QPADRPIPRP PAPRPGTARP 181 GVPRPGASPG SMPPRPGGAV GGARPPRPGA PRPGGRPGAP GAGRSDAGGG NYRGGGVGAA 241 PGTGFRGRPG GGGGGRPGQR GGAAGAFGRP GGAPRRGRKS KRQKRQEYDS MQAPVVGGVR 301 LPHGNGETIR LARGASLSDF ADKIDANPAA LVQALFNLGE MVTATQSVGD ETLELLGSEM 361 NYNVQVVSPE DEDRELLESF DLSYGEDEGG EEDLQVRPPV VTVMGHVDHG KTRLLDTIRK 421 ANVREAEAGG ITQHIGAYQV AVDLDGSQRL ITFIDTPGHE AFTAMRARGA KATDIAILVV 481 AADDGVMPQT VEAINHAQAA DVPIVVAVNK IDKEGADPAK IRGQLTEYGL VPEEFGGDTM 541 FVDISAKQGT NIEALEEAVL LTADAALDLR ANPDMEAQGV AIEAHLDRGR GPVATVLVQR 601 GTLRVGDSVV AGDAYGRVRR MVDEHGEDVE VALPSRPVQV IGFTSVPGAG DNFLVVDEDR 661 IARQIADRRS ARKRNALAAR SRKRISLEDL DSALKETSQL NLILKGDNAG TVEALEEALM 721 GIQVDDEVVL RVIDRGVGGI TETNVNLASA SDAVIIGFNV RAEGKATELA SREGVEIRYY 781 SVIYQAIDEI EQALRGLLKP IYEENQLGRA EIRALFRSSK VGLIAGCLVT SGVMRRNAKA 841 RLLRDNIVVA ENLSIASLRR EKDDVTEVRD GFECGLTLGY ADIKEGDVIE SYELVQKERA //