LOCUS SIU01430.1 410 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE ACYL-CoA DEHYDROGENASE FADE21 protein. ACCESSION LT708304-2844 PROTEIN_ID SIU01430.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="fadE21" /locus_tag="BQ2027_MB2812C" /note="Mb2812c, fadE21, len: 410 aa. Equivalent to Rv2789c, len: 410 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 410 aa overlap). Probable fadE21, acyl-CoA dehydrogenase (EC 1.3.99.-), similar to many e.g. P45857|ACDB_BACSU|MMGC from Bacillus subtilis (379 aa), FASTA scores: opt: 689, E(): 9.3e-37, (35.75% identity in 400 aa overlap); Q9K6D1|ACDA|BH3798 from Bacillus halodurans (380 aa), FASTA scores: opt: 679, E(): 4.1e-36, (37.3% identity in 405 aa overlap); Q06319|ACDS_MEGEL from Megasphaera elsdenii (383 aa), FASTA scores: opt: 650, E(): 3e-34, (37.7% identity in 334 aa overlap); etc. Contains acyl-CoA dehydrogenases signature 1 (PS00072). BELONGS TO THE ACYL-COA DEHYDROGENASES FAMILY. Protein product from Mb2812c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2812c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y270" /db_xref="InterPro:IPR006089" /db_xref="InterPro:IPR006091" /db_xref="InterPro:IPR009075" /db_xref="InterPro:IPR009100" /db_xref="InterPro:IPR013786" /db_xref="InterPro:IPR036250" /db_xref="InterPro:IPR037069" /db_xref="UniProtKB/TrEMBL:A0A1R3Y270" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MFEWSDTDLM VRDAVRQFID KEIRPHQDAL ETGELSPYPI ARKLFSQFGL DVLLAESVNQ 61 MLDGERAKRE KRDSSGSFGL ADQASMVAVL VSELAGVSIG LLSTVAVSLG LGAATIMSRG 121 TLAQQERWVP TLVTLEKIAA WAITEPDSGS DAFGGMKTHV TRDGEDYILN GHKTFITNGP 181 YADVLVVYAK LADGEPASDW RNRPVLVFVL DAGMPGLTQG KPFKKMGMMS SPTGELFFDN 241 VRLTPDRLLC AEGDGRDSAR ANFAVERLGV ALMSLGIINE CHRLCVDYAK TRTLWGRNIG 301 QFQLIQLKLA KMEVARINVQ NMVFQAIERL KAGKQLTLAE ASAIKLYSSE AATDVAMEAV 361 QLFGGNGYMA EYRVEQLARD AKSLMIYAGS NEVQVTHIAK GLLGEPASRA //