LOCUS       SIU01427.1               331 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 probable
            bifunctional fad synthetase/riboflavin biosynthesis protein
            ribf: riboflavin kinase (flavokinase) + fmn adenylyltransferase
            (fad pyrophosphorylase) (fad synthetase)(fad diphosphorylase)
            (flavin adenine dinucleotide synthetase) protein.
ACCESSION   LT708304-2841
PROTEIN_ID  SIU01427.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="ribF"
                     /locus_tag="BQ2027_MB2809C"
                     /note="Mb2809c, ribF, len: 331 aa. Equivalent to Rv2786c,
                     len: 331 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 331 aa overlap). Probable ribF, FAD
                     synthetase/riboflavin biosynthesis protein, bifunctional
                     enzyme (EC 2.7.1.26; 2.7.7.2), equivalent to
                     O32968|RIBF|ML0852 RIBOFLAVIN KINASE from Mycobacterium
                     leprae (331 aa), FASTA scores: opt: 1923, E(): 2.3e-115,
                     (87.45% identity in 327 aa overlap). Also highly similar
                     to many e.g. Q59263|RIBF_CORAM from Corynebacterium
                     ammoniagenes (Brevibacterium ammoniagenes) (338 aa), FASTA
                     scores: opt: 899, E(): 5.7e-50, (45.8% identity in 321 aa
                     overlap); Q9Z530|SC9F2.05c from Streptomyces coelicolor
                     (318 aa), FASTA scores: opt: 862, E(): 1.3e-47, (52.45%
                     identity in 324 aa overlap);
                     P08391|RIBF_ECOLI|B0025|Z0029\ECS0028 from Escherichia
                     coli strains K12 and O157:H7 (313 aa), FASTA scores: opt:
                     517, E(): 1.3e-25, (36.05% identity in 305 aa overlap);
                     etc. Protein product from Mb2809c detected using shotgun
                     mass spectrometry and SWATH mass spectrometry. Mb2809c
                     found to be expressed during exponential growth in
                     Sauton's minimal media by RNA-sequencing (TPM > 20)."
                     /db_xref="GOA:A0A1R3Y2C3"
                     /db_xref="InterPro:IPR002606"
                     /db_xref="InterPro:IPR014729"
                     /db_xref="InterPro:IPR015864"
                     /db_xref="InterPro:IPR015865"
                     /db_xref="InterPro:IPR023465"
                     /db_xref="InterPro:IPR023468"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3Y2C3"
BEGIN
        1 MRRRLAIVQR WRGQDEIPTD WGRCVLTIGV FDGVHRGHAE LIAHAVKAGR ARGVPAVLMT
       61 FDPHPMEVVY PGSHPAQLTT LTRRAELVQD LGIEVFLVMP FTTDFMKLTP DRFIHELLVE
      121 HLHVVEVVVG ENFTFGKKAA GNVDTLRRAG ERFGFAVESM SLVSEHHSNE TVTFSSTYIR
      181 SCVDAGDMVA AMEALGRPHR VEGVVVRGEG RGAELGFPTA NVAPPMYSAI PADGVYAAWF
      241 TVLGHGPVTG TVVPGERYQA AVSVGTNPTF SGRTRTVEAF VLDTTADLYG QHVALDFVGR
      301 IRGQKKFESV RQLVAAMGAD TERARDLLST G
//