LOCUS SIU01427.1 331 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable bifunctional fad synthetase/riboflavin biosynthesis protein ribf: riboflavin kinase (flavokinase) + fmn adenylyltransferase (fad pyrophosphorylase) (fad synthetase)(fad diphosphorylase) (flavin adenine dinucleotide synthetase) protein. ACCESSION LT708304-2841 PROTEIN_ID SIU01427.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="ribF" /locus_tag="BQ2027_MB2809C" /note="Mb2809c, ribF, len: 331 aa. Equivalent to Rv2786c, len: 331 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 331 aa overlap). Probable ribF, FAD synthetase/riboflavin biosynthesis protein, bifunctional enzyme (EC 2.7.1.26; 2.7.7.2), equivalent to O32968|RIBF|ML0852 RIBOFLAVIN KINASE from Mycobacterium leprae (331 aa), FASTA scores: opt: 1923, E(): 2.3e-115, (87.45% identity in 327 aa overlap). Also highly similar to many e.g. Q59263|RIBF_CORAM from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (338 aa), FASTA scores: opt: 899, E(): 5.7e-50, (45.8% identity in 321 aa overlap); Q9Z530|SC9F2.05c from Streptomyces coelicolor (318 aa), FASTA scores: opt: 862, E(): 1.3e-47, (52.45% identity in 324 aa overlap); P08391|RIBF_ECOLI|B0025|Z0029\ECS0028 from Escherichia coli strains K12 and O157:H7 (313 aa), FASTA scores: opt: 517, E(): 1.3e-25, (36.05% identity in 305 aa overlap); etc. Protein product from Mb2809c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2809c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:A0A1R3Y2C3" /db_xref="InterPro:IPR002606" /db_xref="InterPro:IPR014729" /db_xref="InterPro:IPR015864" /db_xref="InterPro:IPR015865" /db_xref="InterPro:IPR023465" /db_xref="InterPro:IPR023468" /db_xref="UniProtKB/TrEMBL:A0A1R3Y2C3" BEGIN 1 MRRRLAIVQR WRGQDEIPTD WGRCVLTIGV FDGVHRGHAE LIAHAVKAGR ARGVPAVLMT 61 FDPHPMEVVY PGSHPAQLTT LTRRAELVQD LGIEVFLVMP FTTDFMKLTP DRFIHELLVE 121 HLHVVEVVVG ENFTFGKKAA GNVDTLRRAG ERFGFAVESM SLVSEHHSNE TVTFSSTYIR 181 SCVDAGDMVA AMEALGRPHR VEGVVVRGEG RGAELGFPTA NVAPPMYSAI PADGVYAAWF 241 TVLGHGPVTG TVVPGERYQA AVSVGTNPTF SGRTRTVEAF VLDTTADLYG QHVALDFVGR 301 IRGQKKFESV RQLVAAMGAD TERARDLLST G //