LOCUS SIU01423.1 438 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE ZINC PROTEASE PEPR protein. ACCESSION LT708304-2837 PROTEIN_ID SIU01423.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="pepR" /locus_tag="BQ2027_MB2805C" /note="Mb2805c, pepR, len: 438 aa. Equivalent to Rv2782c, len: 438 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 438 aa overlap). Probable pepR, protease/peptidase (EC 3.4.99.-), equivalent to O32965|YR82_MYCLE|ML0855|MLCB22.26c HYPOTHETICAL ZINC PROTEASE from Mycobacterium leprae (445 aa), FASTA scores: opt: 2346, E(): 4.3e-146, (84.3% identity in 421 aa overlap). Also highly similar to others e.g. O86835|YA12_STRCO|SC9A10.02 from Streptomyces coelicolor (459 aa), FASTA scores: opt: 1394, E(): 1.1e-83, (51.9% identity in 416 aa overlap); Q04805|YMXG_BACSU|YMXG from Bacillus subtilis (409 aa), FASTA scores: opt: 1014, E(): 7.9e-59, (37.55% identity in 410 aa overlap); Q9KA85|BH2405 from Bacillus halodurans (413 aa), FASTA scores: opt: 967, E(): 9.6e-56, (38.6% identity in 417 aa overlap); etc. Contains PS00143 Insulinase family, zinc-binding region signature. BELONGS TO PEPTIDASE FAMILY M16, ALSO KNOWN AS THE INSULINASE FAMILY. COFACTOR: REQUIRES DIVALENT CATIONS FOR ACTIVITY. BINDS ZINC. Protein product from Mb2805c detected using SWATH mass spectrometry. Mb2805c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P0A5S9" /db_xref="InterPro:IPR001431" /db_xref="InterPro:IPR007863" /db_xref="InterPro:IPR011249" /db_xref="InterPro:IPR011765" /db_xref="UniProtKB/Swiss-Prot:P0A5S9" BEGIN 1 MPRRSPADPA AALAPRRTTL PGGLRVVTEF LPAVHSASVG VWVGVGSRDE GATVAGAAHF 61 LEHLLFKSTP TRSAVDIAQA MDAVGGELNA FTAKEHTCYY AHVLGSDLPL AVDLVADVVL 121 NGRCAADDVE VERDVVLEEI AMRDDDPEDA LADMFLAALF GDHPVGRPVI GSAQSVSVMT 181 RAQLQSFHLR RYTPERMVVA AAGNVDHDGL VALVREHFGS RLVRGRRPVA PRKGTGRVNG 241 SPRLTLVSRD AEQTHVSLGI RTPGRGWEHR WALSVLHTAL GGGLSSRLFQ EVRETRGLAY 301 SVYSALDLFA DSGALSVYAA CLPERFADVM RVTADVLESV ARDGITEAEC GIAKGSLRGG 361 LVLGLEDSSS RMSRLGRSEL NYGKHRSIEH TLRQIEQVTV EEVNAVARHL LSRRYGAAVL 421 GPHGSKRSLP QQLRAMVG //