LOCUS SIU01420.1 132 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 SECRETED L-ALANINE DEHYDROGENASE ALDa [FIRST PART] (40 KDA ANTIGEN) (TB43) protein. ACCESSION LT708304-2834 PROTEIN_ID SIU01420.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="alda" /locus_tag="BQ2027_MB2802" /note="Mb2802, alda, len: 132 aa. Similar to 5' end of Rv2780, len: 371 aa, from Mycobacterium tuberculosis strain H37Rv, (98.9% identity in 89 aa overlap). ald, secreted L-alanine dehydrogenase (EC 1.4.1.1) (40 kd antigen) (see first citations below); equivalent to Q9CBV6|ALD|ML1532 L-ALANINE DEHYDROGENASE from Mycobacterium leprae (371 aa), FASTA scores: opt: 2081, E(): 4e-115, (85.45% identity in 371 aa overlap). Also highly similar to others e.g. Q9S227|SCI51.13c from Streptomyces coelicolor (371 aa), FASTA scores: opt: 1575, E(): 2.3e-85, (66.05% identity in 371 aa overlap); Q9K827|BH3180 from Bacillus halodurans (371 aa), FASTA scores: opt: 1341, E(): 1.4e-71, (56.45% identity in 372 aa overlap); Q9RT70|DR1895 from Deinococcus radiodurans (390 aa), FASTA scores: opt: 1319, E(): 2.8e-70, (54.2% identity in 371 aa overlap); etc. Contains PS00836 and PS00837 Alanine dehydrogenase & pyridine nucleotide transhydrogenase signature 1 and 2. REMARK-M.bovis-M.tuberculosis: In Mycobacterium tuberculosis strain H37Rv, ald exists as a single gene. In Mycobacterium bovis, a frameshift due to a single base deletion (a-*) splits ald into 2 parts, alda and aldb. Mb2802 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:A0A1R3Y257" /db_xref="InterPro:IPR007886" /db_xref="InterPro:IPR008141" /db_xref="InterPro:IPR008142" /db_xref="UniProtKB/TrEMBL:A0A1R3Y257" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MRVGIPTETK NNEFRVAITP AGVAELTRRG HEVLIQAGAG EGSAITDADF KAAGAQLVGT 61 ADQVWADADL LLKVKEPIAA EYGRLRHGRS CSRSCIWPRH VLAPMRCWIP APRQLPTRPS 121 RPPTAHYPCL PR //