LOCUS       SIU01420.1               132 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 SECRETED
            L-ALANINE DEHYDROGENASE ALDa [FIRST PART] (40 KDA ANTIGEN)
            (TB43) protein.
ACCESSION   LT708304-2834
PROTEIN_ID  SIU01420.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="alda"
                     /locus_tag="BQ2027_MB2802"
                     /note="Mb2802, alda, len: 132 aa. Similar to 5' end of
                     Rv2780, len: 371 aa, from Mycobacterium tuberculosis
                     strain H37Rv, (98.9% identity in 89 aa overlap). ald,
                     secreted L-alanine dehydrogenase (EC 1.4.1.1) (40 kd
                     antigen) (see first citations below); equivalent to
                     Q9CBV6|ALD|ML1532 L-ALANINE DEHYDROGENASE from
                     Mycobacterium leprae (371 aa), FASTA scores: opt: 2081,
                     E(): 4e-115, (85.45% identity in 371 aa overlap). Also
                     highly similar to others e.g. Q9S227|SCI51.13c from
                     Streptomyces coelicolor (371 aa), FASTA scores: opt: 1575,
                     E(): 2.3e-85, (66.05% identity in 371 aa overlap);
                     Q9K827|BH3180 from Bacillus halodurans (371 aa), FASTA
                     scores: opt: 1341, E(): 1.4e-71, (56.45% identity in 372
                     aa overlap); Q9RT70|DR1895 from Deinococcus radiodurans
                     (390 aa), FASTA scores: opt: 1319, E(): 2.8e-70, (54.2%
                     identity in 371 aa overlap); etc. Contains PS00836 and
                     PS00837 Alanine dehydrogenase & pyridine nucleotide
                     transhydrogenase signature 1 and 2.
                     REMARK-M.bovis-M.tuberculosis: In Mycobacterium
                     tuberculosis strain H37Rv, ald exists as a single gene. In
                     Mycobacterium bovis, a frameshift due to a single base
                     deletion (a-*) splits ald into 2 parts, alda and aldb.
                     Mb2802 found to be expressed during exponential growth in
                     Sauton's minimal media by RNA-sequencing (TPM > 20)."
                     /db_xref="GOA:A0A1R3Y257"
                     /db_xref="InterPro:IPR007886"
                     /db_xref="InterPro:IPR008141"
                     /db_xref="InterPro:IPR008142"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3Y257"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MRVGIPTETK NNEFRVAITP AGVAELTRRG HEVLIQAGAG EGSAITDADF KAAGAQLVGT
       61 ADQVWADADL LLKVKEPIAA EYGRLRHGRS CSRSCIWPRH VLAPMRCWIP APRQLPTRPS
      121 RPPTAHYPCL PR
//