LOCUS       SIU01404.1               263 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            THYMIDYLATE SYNTHASE THYA (TS) (TSASE) protein.
ACCESSION   LT708304-2818
PROTEIN_ID  SIU01404.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="thyA"
                     /locus_tag="BQ2027_MB2786C"
                     /note="Mb2786c, thyA, len: 263 aa. Equivalent to Rv2764c,
                     len: 263 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 263 aa overlap). Probable thyA,
                     thymidylate synthase (EC 2.1.1.45), equivalent to
                     Q9CBW0|TYSY_MYCLE|THYA|ML1519 THYMIDYLATE SYNTHASE from
                     Mycobacterium leprae (266 aa), FASTA scores: opt: 1602,
                     E(): 5.9e-102, (85.5% identity in 262 aa overlap). Also
                     highly similar to many e.g.
                     P00470|TYSY_ECOLI|B2827|Z4144|ECS3684|BAB37107|AAG57938
                     from Escherichia coli strains K12 and O157:H7 (264 aa),
                     FASTA scores: opt: 1309, E(): 5.9e-82, (66.65% identity in
                     261 aa overlap); P48464|TYSY_SHIFL|THYA from Shigella
                     flexneri (264 aa), FASTA scores: opt: 1303, E(): 1.5e-81,
                     (65.9% identity in 261 aa overlap);
                     P54081|TYSB_BACAM|THYB|THYBA from Bacillus
                     amyloliquefaciens (264 aa), FASTA scores: opt: 1235, E():
                     6.7e-77, (66.65% identity in 261 aa overlap); etc.
                     Contains PS00091 Thymidylate synthase active site. BELONGS
                     TO THE THYMIDYLATE SYNTHASE FAMILY. Protein product from
                     Mb2786c detected using SWATH mass spectrometry. Mb2786c
                     found to be expressed during exponential growth in
                     Sauton's minimal media by RNA-sequencing (TPM > 20)."
                     /db_xref="GOA:P67045"
                     /db_xref="InterPro:IPR000398"
                     /db_xref="InterPro:IPR020940"
                     /db_xref="InterPro:IPR023451"
                     /db_xref="InterPro:IPR036926"
                     /db_xref="UniProtKB/Swiss-Prot:P67045"
BEGIN
        1 MTPYEDLLRF VLETGTPKSD RTGTGTRSLF GQQMRYDLSA GFPLLTTKKV HFKSVAYELL
       61 WFLRGDSNIG WLHEHGVTIW DEWASDTGEL GPIYGVQWRS WPAPSGEHID QISAALDLLR
      121 TDPDSRRIIV SAWNVGEIER MALPPCHAFF QFYVADGRLS CQLYQRSADL FLGVPFNIAS
      181 YALLTHMMAA QAGLSVGEFI WTGGDCHIYD NHVEQVRLQL SREPRPYPKL LLADRDSIFE
      241 YTYEDIVVKN YDPHPAIKAP VAV
//