LOCUS SIU01404.1 263 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE THYMIDYLATE SYNTHASE THYA (TS) (TSASE) protein. ACCESSION LT708304-2818 PROTEIN_ID SIU01404.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="thyA" /locus_tag="BQ2027_MB2786C" /note="Mb2786c, thyA, len: 263 aa. Equivalent to Rv2764c, len: 263 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 263 aa overlap). Probable thyA, thymidylate synthase (EC 2.1.1.45), equivalent to Q9CBW0|TYSY_MYCLE|THYA|ML1519 THYMIDYLATE SYNTHASE from Mycobacterium leprae (266 aa), FASTA scores: opt: 1602, E(): 5.9e-102, (85.5% identity in 262 aa overlap). Also highly similar to many e.g. P00470|TYSY_ECOLI|B2827|Z4144|ECS3684|BAB37107|AAG57938 from Escherichia coli strains K12 and O157:H7 (264 aa), FASTA scores: opt: 1309, E(): 5.9e-82, (66.65% identity in 261 aa overlap); P48464|TYSY_SHIFL|THYA from Shigella flexneri (264 aa), FASTA scores: opt: 1303, E(): 1.5e-81, (65.9% identity in 261 aa overlap); P54081|TYSB_BACAM|THYB|THYBA from Bacillus amyloliquefaciens (264 aa), FASTA scores: opt: 1235, E(): 6.7e-77, (66.65% identity in 261 aa overlap); etc. Contains PS00091 Thymidylate synthase active site. BELONGS TO THE THYMIDYLATE SYNTHASE FAMILY. Protein product from Mb2786c detected using SWATH mass spectrometry. Mb2786c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:P67045" /db_xref="InterPro:IPR000398" /db_xref="InterPro:IPR020940" /db_xref="InterPro:IPR023451" /db_xref="InterPro:IPR036926" /db_xref="UniProtKB/Swiss-Prot:P67045" BEGIN 1 MTPYEDLLRF VLETGTPKSD RTGTGTRSLF GQQMRYDLSA GFPLLTTKKV HFKSVAYELL 61 WFLRGDSNIG WLHEHGVTIW DEWASDTGEL GPIYGVQWRS WPAPSGEHID QISAALDLLR 121 TDPDSRRIIV SAWNVGEIER MALPPCHAFF QFYVADGRLS CQLYQRSADL FLGVPFNIAS 181 YALLTHMMAA QAGLSVGEFI WTGGDCHIYD NHVEQVRLQL SREPRPYPKL LLADRDSIFE 241 YTYEDIVVKN YDPHPAIKAP VAV //