LOCUS SIU01403.1 159 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 dihydrofolate reductase dfra (dhfr) (tetrahydrofolate dehydrogenase) protein. ACCESSION LT708304-2817 PROTEIN_ID SIU01403.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="dfrA" /locus_tag="BQ2027_MB2785C" /note="Mb2785c, dfrA, len: 159 aa. Equivalent to Rv2763c, len: 159 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 159 aa overlap). Probable dfrA (alternate gene name: folA), dihydrofolate reductase (EC 1.5.1.3), equivalent to O30463|FOLA DIHYDROFOLATE REDUCTASE from Mycobacterium avium (see citation below) (181 aa), FASTA scores: opt: 802, E(): 4.5e-48, (70.2% identity in 161 aa overlap); and Q9CBW1|FOLA|ML1518 DIHYDROFOLATE REDUCTASE from Mycobacterium leprae (165 aa), FASTA scores: opt: 782, E(): 1e-46, (70.55% identity in 163 aa overlap). Also highly similar to many e.g. Q9K168|DYR_NEIMB|FOLA|NMB0308 from Neisseria meningitidis (serogroup B) (162 aa), FASTA scores: opt: 469, E(): 3.8e-25, (46.65% identity in 163 aa overlap); P12833|DYR3_SALTY|DHFRIII from Salmonella typhimurium (162 aa), FASTA scores: opt: 367, E(): 4e-18, (45.4% identity in 141 aa overlap); Q59408|DYRC_ECOLI|DHFRXIII from Escherichia coli strain RA33.2 (165 aa), FASTA scores: opt: 313, E(): 2.2e-14, (41.9% identity in 136 aa overlap); etc. Contains PS00075 Dihydrofolate reductase signature. BELONGS TO THE DIHYDROFOLATE REDUCTASE FAMILY. Protein product from Mb2785c detected using SWATH mass spectrometry. Mb2785c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P0A547" /db_xref="InterPro:IPR001796" /db_xref="InterPro:IPR012259" /db_xref="InterPro:IPR017925" /db_xref="InterPro:IPR024072" /db_xref="UniProtKB/Swiss-Prot:P0A547" BEGIN 1 MVGLIWAQAT SGVIGRGGDI PWRLPEDQAH FREITMGHTI VMGRRTWDSL PAKVRPLPGR 61 RNVVLSRQAD FMASGAEVVG SLEEALTSPE TWVIGGGQVY ALALPYATRC EVTEVDIGLP 121 REAGDALAPV LDETWRGETG EWRFSRSGLR YRLYSYHRS //