LOCUS       SIU01403.1               159 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 dihydrofolate
            reductase dfra (dhfr) (tetrahydrofolate dehydrogenase)
            protein.
ACCESSION   LT708304-2817
PROTEIN_ID  SIU01403.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="dfrA"
                     /locus_tag="BQ2027_MB2785C"
                     /note="Mb2785c, dfrA, len: 159 aa. Equivalent to Rv2763c,
                     len: 159 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 159 aa overlap). Probable dfrA
                     (alternate gene name: folA), dihydrofolate reductase (EC
                     1.5.1.3), equivalent to O30463|FOLA DIHYDROFOLATE
                     REDUCTASE from Mycobacterium avium (see citation below)
                     (181 aa), FASTA scores: opt: 802, E(): 4.5e-48, (70.2%
                     identity in 161 aa overlap); and Q9CBW1|FOLA|ML1518
                     DIHYDROFOLATE REDUCTASE from Mycobacterium leprae (165
                     aa), FASTA scores: opt: 782, E(): 1e-46, (70.55% identity
                     in 163 aa overlap). Also highly similar to many e.g.
                     Q9K168|DYR_NEIMB|FOLA|NMB0308 from Neisseria meningitidis
                     (serogroup B) (162 aa), FASTA scores: opt: 469, E():
                     3.8e-25, (46.65% identity in 163 aa overlap);
                     P12833|DYR3_SALTY|DHFRIII from Salmonella typhimurium (162
                     aa), FASTA scores: opt: 367, E(): 4e-18, (45.4% identity
                     in 141 aa overlap); Q59408|DYRC_ECOLI|DHFRXIII from
                     Escherichia coli strain RA33.2 (165 aa), FASTA scores:
                     opt: 313, E(): 2.2e-14, (41.9% identity in 136 aa
                     overlap); etc. Contains PS00075 Dihydrofolate reductase
                     signature. BELONGS TO THE DIHYDROFOLATE REDUCTASE FAMILY.
                     Protein product from Mb2785c detected using SWATH mass
                     spectrometry. Mb2785c found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:P0A547"
                     /db_xref="InterPro:IPR001796"
                     /db_xref="InterPro:IPR012259"
                     /db_xref="InterPro:IPR017925"
                     /db_xref="InterPro:IPR024072"
                     /db_xref="UniProtKB/Swiss-Prot:P0A547"
BEGIN
        1 MVGLIWAQAT SGVIGRGGDI PWRLPEDQAH FREITMGHTI VMGRRTWDSL PAKVRPLPGR
       61 RNVVLSRQAD FMASGAEVVG SLEEALTSPE TWVIGGGQVY ALALPYATRC EVTEVDIGLP
      121 REAGDALAPV LDETWRGETG EWRFSRSGLR YRLYSYHRS
//