LOCUS SIU01395.1 540 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE TYPE I RESTRICTION/MODIFICATION SYSTEM DNA METHYLASE HSDM (M PROTEIN) (DNA METHYLTRANSFERASE) protein. ACCESSION LT708304-2809 PROTEIN_ID SIU01395.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="hsdM" /locus_tag="BQ2027_MB2777C" /note="Mb2777c, hsdM, len: 540 aa. Equivalent to Rv2756c, len: 540 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 540 aa overlap). Possible hsdM, type I restriction/modification system DNA methylase (M protein) (EC 2.1.1.-), highly similar to others e.g. Q9P9X8|XF2742 from Xylella fastidiosa (519 aa), FASTA scores: opt: 1613, E(): 1.9e-96, (52.3% identity in 543 aa overlap); O34139|HSDM from Klebsiella pneumoniae (539 aa), FASTA scores: opt: 1267, E(): 4.4e-74, (45.9% identity in 549 aa overlap); P72418|STY|SBLI|HSDM from Salmonella typhimurium (539 aa), FASTA scores: opt: 1263, E(): 8e-74, (45.7% identity in 549 aa overlap); etc. Possible alternative start site (GTG) overlapping with termination codon of previous ORF 90 bp upstream. Note that the corresponding endonuclease (M protein) does not appear to be present in Mycobacterium tuberculosis. Protein product from Mb2777c detected using SWATH mass spectrometry. Mb2777c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y434" /db_xref="InterPro:IPR003356" /db_xref="InterPro:IPR022749" /db_xref="InterPro:IPR029063" /db_xref="InterPro:IPR038333" /db_xref="UniProtKB/TrEMBL:A0A1R3Y434" BEGIN 1 MPPRKKQAPQ APSTMKELKD TLWKAADKLR GSLSASQYKD VILGLVFLKY VSDAYDERRE 61 AIRAELAAEG MEESQIEDLI DDPEQYQGYG VFVVPVSARW KFLAENTKGK PAVGGEPAKN 121 IGQLIDEAMD AVMKANPTLG GTLPRLYNKD NIDQRRLGEL IDLFNSARFS RQGEHRARDL 181 MGEVYEYFLG NFARAEGKRG GEFFTPPSVV KVIVEVLEPS SGRVYDPCCG SGGMFVQTEK 241 FIYEHDGDPK DVSIYGQESI EETWRMAKMN LAIHGIDNKG LGARWSDTFA RDQHPDVQMD 301 YVMANPPFNI KDWARNEEDP RWRFGVPPAN NANYAWIQHI LYKLAPGGRA GVVMANGSMS 361 SNSNGEGDIR AQIVEADLVS CMVALPTQLF RSTGIPVCLW FFAKDKAAGK QGSIDRCGQV 421 LFIDARELGD LVDRAERALT NEEIVRIGDT FHAWRGSKSA AVKGIMYEDV PGFCKSATLA 481 EIKATDYALT PGRYVGTPAV EDDGEPIDEK MARLSKALLE AFDESARLER VVREQLGRLR //