LOCUS       SIU01395.1               540 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE
            TYPE I RESTRICTION/MODIFICATION SYSTEM DNA METHYLASE HSDM
            (M PROTEIN) (DNA METHYLTRANSFERASE) protein.
ACCESSION   LT708304-2809
PROTEIN_ID  SIU01395.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="hsdM"
                     /locus_tag="BQ2027_MB2777C"
                     /note="Mb2777c, hsdM, len: 540 aa. Equivalent to Rv2756c,
                     len: 540 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (99.8% identity in 540 aa overlap). Possible hsdM, type I
                     restriction/modification system DNA methylase (M protein)
                     (EC 2.1.1.-), highly similar to others e.g. Q9P9X8|XF2742
                     from Xylella fastidiosa (519 aa), FASTA scores: opt: 1613,
                     E(): 1.9e-96, (52.3% identity in 543 aa overlap);
                     O34139|HSDM from Klebsiella pneumoniae (539 aa), FASTA
                     scores: opt: 1267, E(): 4.4e-74, (45.9% identity in 549 aa
                     overlap); P72418|STY|SBLI|HSDM from Salmonella typhimurium
                     (539 aa), FASTA scores: opt: 1263, E(): 8e-74, (45.7%
                     identity in 549 aa overlap); etc. Possible alternative
                     start site (GTG) overlapping with termination codon of
                     previous ORF 90 bp upstream. Note that the corresponding
                     endonuclease (M protein) does not appear to be present in
                     Mycobacterium tuberculosis. Protein product from Mb2777c
                     detected using SWATH mass spectrometry. Mb2777c found to
                     be expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing."
                     /db_xref="GOA:A0A1R3Y434"
                     /db_xref="InterPro:IPR003356"
                     /db_xref="InterPro:IPR022749"
                     /db_xref="InterPro:IPR029063"
                     /db_xref="InterPro:IPR038333"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3Y434"
BEGIN
        1 MPPRKKQAPQ APSTMKELKD TLWKAADKLR GSLSASQYKD VILGLVFLKY VSDAYDERRE
       61 AIRAELAAEG MEESQIEDLI DDPEQYQGYG VFVVPVSARW KFLAENTKGK PAVGGEPAKN
      121 IGQLIDEAMD AVMKANPTLG GTLPRLYNKD NIDQRRLGEL IDLFNSARFS RQGEHRARDL
      181 MGEVYEYFLG NFARAEGKRG GEFFTPPSVV KVIVEVLEPS SGRVYDPCCG SGGMFVQTEK
      241 FIYEHDGDPK DVSIYGQESI EETWRMAKMN LAIHGIDNKG LGARWSDTFA RDQHPDVQMD
      301 YVMANPPFNI KDWARNEEDP RWRFGVPPAN NANYAWIQHI LYKLAPGGRA GVVMANGSMS
      361 SNSNGEGDIR AQIVEADLVS CMVALPTQLF RSTGIPVCLW FFAKDKAAGK QGSIDRCGQV
      421 LFIDARELGD LVDRAERALT NEEIVRIGDT FHAWRGSKSA AVKGIMYEDV PGFCKSATLA
      481 EIKATDYALT PGRYVGTPAV EDDGEPIDEK MARLSKALLE AFDESARLER VVREQLGRLR
//