LOCUS SIU01393.1 250 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable thymidylate synthase thyx (ts) (tsase) protein. ACCESSION LT708304-2807 PROTEIN_ID SIU01393.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="thyx" /locus_tag="BQ2027_MB2775C" /note="Mb2775c, -, len: 250 aa. Equivalent to Rv2754c, len: 250 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 250 aa overlap). Conserved hypothetical ala-rich protein, equivalent to Q9CBW3|YF14_MYCLE|ML1514 HYPOTHETICAL 28.0 KDA PROTEIN from Mycobacterium leprae (254 aa), FASTA scores: opt: 1351, E(): 1e-84, (81.5% identity in 254 aa overlap). Also highly similar to 40111|YDAP_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (250 aa), FASTA scores: opt: 1071, E(): 1.2e-65, (62.85% identity in 245 aa overlap); Q05259|VG48_BPML5 GENE 48 PROTEIN (GP48) from Mycobacteriophage L5 (243 aa), FASTA scores: opt: 610, E(): 3.2e-34, (49.55% identity in 220 aa overlap); O64238|VG48_BPMD2 GENE 48 PROTEIN (GP48) from Mycobacteriophage D29 (235 aa), FASTA scores: opt: 593, E(): 4.5e-33, (46.95% identity in 245 aa overlap); etc. BELONGS TO THE THY1 FAMILY. Protein product from Mb2775c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2775c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P66931" /db_xref="InterPro:IPR003669" /db_xref="InterPro:IPR036098" /db_xref="UniProtKB/Swiss-Prot:P66931" BEGIN 1 MAETAPLRVQ LIAKTDFLAP PDVPWTTDAD GGPALVEFAG RACYQSWSKP NPKTATNAGY 61 LRHIIDVGHF SVLEHASVSF YITGISRSCT HELIRHRHFS YSQLSQRYVP EKDSRVVVPP 121 GMEDDADLRH ILTEAADAAR ATYSELLAKL EAKFADQPNA ILRRKQARQA ARAVLPNATE 181 TRIVVTGNYR AWRHFIAMRA SEHADVEIRR LAIECLRQLA AVAPAVFADF EVTTLADGTE 241 VATSPLATEA //