LOCUS SIU01392.1 300 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE DIHYDRODIPICOLINATE SYNTHASE DAPA (DHDPS) (DIHYDRODIPICOLINATE SYNTHETASE) protein. ACCESSION LT708304-2806 PROTEIN_ID SIU01392.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="dapA" /locus_tag="BQ2027_MB2774C" /note="Mb2774c, dapA, len: 300 aa. Equivalent to Rv2753c, len: 300 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 300 aa overlap). Probable dapA, dihydrodipicolinate synthase (EC 4.2.1.52), equivalent to Q9CBW4|DAPA_MYCLE|ML1513 DIHYDRODIPICOLINATE SYNTHASE from Mycobacterium leprae (300 aa), FASTA scores: opt: 1699, E(): 2.2e-98, (86.65% identity in 300 aa overlap). Also highly similar to many e.g. P19808|DAPA_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (301 aa), FASTA scores: opt: 1089, E(): 2e-60, (58.7% identity in 288 aa overlap); O86841|DAPA_STRCO|SC9A10.08 from Streptomyces coelicolor (299 aa), FASTA scores: opt: 1044, E(): 1.3e-57, (55.75% identity in 287 aa overlap); P05640|DAPA_ECOLI (292 aa), FASTA scores: opt: 515, E(): 0, (33.8% identity in 287 aa overlap); etc. Contains PS00665 and PS00666 Dihydrodipicolinate synthetase signatures 1 and 2. BELONGS TO THE DHDPS FAMILY. Protein product from Mb2774c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2774c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:P63946" /db_xref="InterPro:IPR002220" /db_xref="InterPro:IPR005263" /db_xref="InterPro:IPR013785" /db_xref="InterPro:IPR020624" /db_xref="InterPro:IPR020625" /db_xref="UniProtKB/Swiss-Prot:P63946" BEGIN 1 MTTVGFDVAA RLGTLLTAMV TPFSGDGSLD TATAARLANH LVDQGCDGLV VSGTTGESPT 61 TTDGEKIELL RAVLEAVGDR ARVIAGAGTY DTAHSIRLAK ACAAEGAHGL LVVTPYYSKP 121 PQRGLQAHFT AVADATELPM LLYDIPGRSA VPIEPDTIRA LASHPNIVGV KDAKADLHSG 181 AQIMADTGLA YYSGDDALNL PWLAMGATGF ISVIAHLAAG QLRELLSAFG SGDIATARKI 241 NIAVAPLCNA MSRLGGVTLS KAGLRLQGID VGDPRLPQVA ATPEQIDALA ADMRAASVLR //