LOCUS SIU01383.1 270 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 CONSERVED 35 KDA ALANINE RICH PROTEIN protein. ACCESSION LT708304-2797 PROTEIN_ID SIU01383.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="35kd_ag" /locus_tag="BQ2027_MB2765C" /note="Mb2765c, 35kd_ag, len: 270 aa. Equivalent to Rv2744c, len: 270 aa, from Mycobacterium tuberculosis strain H37Rv, (99.6% identity in 270 aa overlap). 35kd_ag, conserved ala-rich protein 35-kd antigen (see citation below). N-terminal part is equivalent to Q49840|MLCB33.06c|B2235_C2_187 HYPOTHETICAL PROTEIN from Mycobacterium leprae (167 aa), FASTA scores: opt: 789, E(): 3.4e-35, (85.05% identity in 147 aa overlap); and C-terminal part equivalent to Q49845|MLCB33.05c|B2235_C3_214 HYPOTHETICAL PROTEIN from Mycobacterium leprae (114 aa), FASTA scores: opt: 465, E(): 3.6e-18, (65.8% identity in 114 aa overlap); note that these two proteins from Mycobacterium leprae are adjacent. Shows some similarity with Q55707||Y617_SYNY3|SLL0617 HYPOTHETICAL 28.9 KDA PROTEIN from Synechocystis sp. strain PCC 6803 (267 aa), FASTA scores: opt: 155, E(): 0.19, (23.4% identity in 252 aa overlap); and C-terminus of Q9L4N1|EMM M PROTEIN from Streptococcus equisimilis (592 aa), FASTA scores: opt: 165, E(): 0.11, (23.45% identity in 260 aa overlap). C-terminus also similar to AAK45945|MT1676 CONSERVED HYPOTHETICAL PROTEIN from Mycobacterium tuberculosis strain CDC1551 (85 aa), FASTA scores: opt: 159, E(): 0.047, (50.9% identity in 55 aa overlap). Protein product from Mb2765c detected using shotgun mass spectrometry. Mb2765c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="InterPro:IPR007157" /db_xref="UniProtKB/TrEMBL:A0A1R3Y211" BEGIN 1 MANPFVKAWK YLMALFSSKI DEHADPKVQI QQAIEEAQRT HQALTQQAAQ VIGNQRQLEM 61 RLNRQLADIE KLQVNVRQAL TLADQATAAG DAAKATEYNN AAEAFAAQLV TAEQSVEDLK 121 TLHDQALSAA AQAKKAVERN AMVLQQKIAE RTQLLSQLEQ AKMQEQVSAS LRSMSELAAP 181 GNTPSLDEVR DKIERRYANA IGSAELAESS VQGRMLEVEQ AGIQMAGHSR LEQIRASMRG 241 EALPAGGTTA TPRPATETSG GAIAEQPYGQ //