LOCUS       SIU01350.1               468 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            SOLUBLE PYRIDINE NUCLEOTIDE TRANSHYDROGENASE STHA (STH)
            (NAD(P)(+) TRANSHYDROGENASE [B-SPECIFIC]) (NICOTINAMIDE
            NUCLEOTIDE TRANSHYDROGENASE) protein.
ACCESSION   LT708304-2764
PROTEIN_ID  SIU01350.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="sthA"
                     /locus_tag="BQ2027_MB2732"
                     /note="Mb2732, sthA, len: 468 aa. Equivalent to Rv2713,
                     len: 468 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 468 aa overlap). Probable sthA,
                     soluble pyridine nucleotide transhydrogenase (EC 1.6.1.1),
                     highly similar to others e.g. Q983E2|MLR8366 from
                     Rhizobium loti (Mesorhizobium loti) (481 aa), FASTA
                     scores: opt: 1447, E(): 4.1e-78, (49.55% identity in 460
                     aa overlap); P27306|STHA_ECOLI|STH|UDHA|B3962 from
                     Escherichia coli strain K12 (465 aa), FASTA scores: opt:
                     1267, E(): 1.7e-67, (43.05% identity in 462 aa overlap);
                     O05139|STHA_PSEFL|STH from Pseudomonas fluorescens (463
                     aa), FASTA scores: opt: 1257, E(): 6.6e-67, (43.8%
                     identity in 461 aa overlap); etc. Also highly similar to
                     CAC46308|SMC00300 PUTATIVE OXIDOREDUCTASE PROTEIN from
                     Rhizobium meliloti (Sinorhizobium meliloti) (467 aa),
                     FASTA scores: opt: 1466, E(): 3e-79, (49.55% identity in
                     462 aa overlap). Shows some similarity to MTCY359.04, E():
                     3.1e-08; MTCY210.05, E(): 3.4e-08. Contains
                     ATP/GTP-binding site motif A (P-loop; PS00017). BELONGS TO
                     THE PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASES CLASS-I.
                     COFACTOR: FAD (BY SIMILARITY). Protein product from Mb2732
                     detected using shotgun mass spectrometry and SWATH mass
                     spectrometry. Mb2732 found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:P66007"
                     /db_xref="InterPro:IPR001100"
                     /db_xref="InterPro:IPR004099"
                     /db_xref="InterPro:IPR016156"
                     /db_xref="InterPro:IPR022962"
                     /db_xref="InterPro:IPR023753"
                     /db_xref="InterPro:IPR036188"
                     /db_xref="UniProtKB/Swiss-Prot:P66007"
BEGIN
        1 MREYDIVVIG SGPGGQKAAI ASAKLGKSVA IVERGRMLGG VCVNTGTIPS KTLREAVLYL
       61 TGMNQRELYG ASYRVKDRIT PADLLARTQH VIGKEVDVVR NQLMRNRVDL IVGHGRFIDP
      121 HTILVEDQAR REKTTVTGDY IIIATGTRPA RPSGVEFDEE RVLDSDGILD LKSLPSSMVV
      181 VGAGVIGIEY ASMFAALGTK VTVVEKRDNM LDFCDPEVVE ALKFHLRDLA VTFRFGEEVT
      241 AVDVGSAGTV TTLASGKQIP AETVMYSAGR QGQTDHLDLH NAGLEVQGRG RIFVDDRFQT
      301 KVDHIYAVGD VIGFPALAAT SMEQGRLAAY HAFGEPTDGI TELQPIGIYS IPEVSYVGAT
      361 EVELTKSSIP YEVGVARYRE LARGQIAGDS YGMLKLLVST EDLKLLGVHI FGTSATEMVH
      421 IGQAVMGCGG SVEYLVDAVF NYPTFSEAYK NAALDVMNKM RALNQFRR
//