LOCUS SIU01350.1 468 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE SOLUBLE PYRIDINE NUCLEOTIDE TRANSHYDROGENASE STHA (STH) (NAD(P)(+) TRANSHYDROGENASE [B-SPECIFIC]) (NICOTINAMIDE NUCLEOTIDE TRANSHYDROGENASE) protein. ACCESSION LT708304-2764 PROTEIN_ID SIU01350.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="sthA" /locus_tag="BQ2027_MB2732" /note="Mb2732, sthA, len: 468 aa. Equivalent to Rv2713, len: 468 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 468 aa overlap). Probable sthA, soluble pyridine nucleotide transhydrogenase (EC 1.6.1.1), highly similar to others e.g. Q983E2|MLR8366 from Rhizobium loti (Mesorhizobium loti) (481 aa), FASTA scores: opt: 1447, E(): 4.1e-78, (49.55% identity in 460 aa overlap); P27306|STHA_ECOLI|STH|UDHA|B3962 from Escherichia coli strain K12 (465 aa), FASTA scores: opt: 1267, E(): 1.7e-67, (43.05% identity in 462 aa overlap); O05139|STHA_PSEFL|STH from Pseudomonas fluorescens (463 aa), FASTA scores: opt: 1257, E(): 6.6e-67, (43.8% identity in 461 aa overlap); etc. Also highly similar to CAC46308|SMC00300 PUTATIVE OXIDOREDUCTASE PROTEIN from Rhizobium meliloti (Sinorhizobium meliloti) (467 aa), FASTA scores: opt: 1466, E(): 3e-79, (49.55% identity in 462 aa overlap). Shows some similarity to MTCY359.04, E(): 3.1e-08; MTCY210.05, E(): 3.4e-08. Contains ATP/GTP-binding site motif A (P-loop; PS00017). BELONGS TO THE PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASES CLASS-I. COFACTOR: FAD (BY SIMILARITY). Protein product from Mb2732 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2732 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P66007" /db_xref="InterPro:IPR001100" /db_xref="InterPro:IPR004099" /db_xref="InterPro:IPR016156" /db_xref="InterPro:IPR022962" /db_xref="InterPro:IPR023753" /db_xref="InterPro:IPR036188" /db_xref="UniProtKB/Swiss-Prot:P66007" BEGIN 1 MREYDIVVIG SGPGGQKAAI ASAKLGKSVA IVERGRMLGG VCVNTGTIPS KTLREAVLYL 61 TGMNQRELYG ASYRVKDRIT PADLLARTQH VIGKEVDVVR NQLMRNRVDL IVGHGRFIDP 121 HTILVEDQAR REKTTVTGDY IIIATGTRPA RPSGVEFDEE RVLDSDGILD LKSLPSSMVV 181 VGAGVIGIEY ASMFAALGTK VTVVEKRDNM LDFCDPEVVE ALKFHLRDLA VTFRFGEEVT 241 AVDVGSAGTV TTLASGKQIP AETVMYSAGR QGQTDHLDLH NAGLEVQGRG RIFVDDRFQT 301 KVDHIYAVGD VIGFPALAAT SMEQGRLAAY HAFGEPTDGI TELQPIGIYS IPEVSYVGAT 361 EVELTKSSIP YEVGVARYRE LARGQIAGDS YGMLKLLVST EDLKLLGVHI FGTSATEMVH 421 IGQAVMGCGG SVEYLVDAVF NYPTFSEAYK NAALDVMNKM RALNQFRR //