LOCUS SIU01340.1 528 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 RNA
POLYMERASE SIGMA FACTOR SIGA (SIGMA-A) protein.
ACCESSION LT708304-2754
PROTEIN_ID SIU01340.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/gene="sigA"
/locus_tag="BQ2027_MB2722"
/standard_name="mysA; rpoV; rpoD"
/note="Mb2722, sigA, len: 528 aa. Equivalent to Rv2703,
len: 528 aa, from Mycobacterium tuberculosis strain H37Rv,
(100.0% identity in 528 aa overlap). sigA (formerly named
mysA, and also known as rpoV or rpoD), RNA polymerase
sigma factor (see citations below), equivalent (but
shorter 55 aa) to Q9S5K3|RPOT (alias Q59532) RNA
POLYMERASE SIGMA FACTOR from Mycobacterium leprae (576
aa), FASTA scores: opt: 2638, E(): 8.6e-115, (80.35%
identity in 535 aa overlap). Also similar to others e.g.
Q59552|MYSA from Mycobacterium smegmatis (466 aa), FASTA
scores: opt: 2259, E(): 2.3e-97, (76.5% identity in 528 aa
overlap); Q45302|SIGA from Corynebacterium glutamicum
(Brevibacterium flavum) (497 aa), FASTA scores: opt: 1972,
E(): 4.3e-84, (67.35% identity in 505 aa overlap);
Q59813|HRDB from Streptomyces aureofaciens (525 aa), FASTA
scores: opt: 1654, E(): 2.1e-69, (67.5% identity in 468 aa
overlap); etc. Contains sigma-70 family signatures 1 and 2
(PS00715 and PS00716). BELONGS TO THE SIGMA-70 FACTOR
FAMILY. Protein product from Mb2722 detected using shotgun
mass spectrometry and SWATH mass spectrometry. Mb2722
found to be expressed during exponential growth in
Sauton's minimal media by RNA-sequencing (TPM > 20)."
/db_xref="GOA:P0A603"
/db_xref="InterPro:IPR000943"
/db_xref="InterPro:IPR007624"
/db_xref="InterPro:IPR007627"
/db_xref="InterPro:IPR007630"
/db_xref="InterPro:IPR009042"
/db_xref="InterPro:IPR012760"
/db_xref="InterPro:IPR013324"
/db_xref="InterPro:IPR013325"
/db_xref="InterPro:IPR014284"
/db_xref="InterPro:IPR028630"
/db_xref="InterPro:IPR036388"
/db_xref="UniProtKB/Swiss-Prot:P0A603"
/experiment="experimental evidence, no additional details
recorded"
BEGIN
1 MAATKASTAT DEPVKRTATK SPAASASGAK TGAKRTAAKS ASGSPPAKRA TKPAARSVKP
61 ASAPQDTTTS TIPKRKTRAA AKSAAAKAPS ARGHATKPRA PKDAQHEAAT DPEDALDSVE
121 ELDAEPDLDV EPGEDLDLDA ADLNLDDLED DVAPDADDDL DSGDDEDHED LEAEAAVAPG
181 QTADDDEEIA EPTEKDKASG DFVWDEDESE ALRQARKDAE LTASADSVRA YLKQIGKVAL
241 LNAEEEVELA KRIEAGLYAT QLMTELSERG EKLPAAQRRD MMWICRDGDR AKNHLLEANL
301 RLVVSLAKRY TGRGMAFLDL IQEGNLGLIR AVEKFDYTKG YKFSTYATWW IRQAITRAMA
361 DQARTIRIPV HMVEVINKLG RIQRELLQDL GREPTPEELA KEMDITPEKV LEIQQYAREP
421 ISLDQTIGDE GDSQLGDFIE DSEAVVAVDA VSFTLLQDQL QSVLDTLSER EAGVVRLRFG
481 LTDGQPRTLD EIGQVYGVTR ERIRQIESKT MSKLRHPSRS QVLRDYLD
//