LOCUS       SIU01339.1               265 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 POLYPHOSPHATE
            GLUCOKINASE PPGK (POLYPHOSPHATE-GLUCOSE PHOSPHOTRANSFERASE)
            protein.
ACCESSION   LT708304-2753
PROTEIN_ID  SIU01339.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="ppgK"
                     /locus_tag="BQ2027_MB2721"
                     /note="Mb2721, ppgK, len: 265 aa. Equivalent to Rv2702,
                     len: 265 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (99.6% identity in 265 aa overlap). ppgK, polyphosphate
                     glucokinase (EC 2.7.1.2) (see citation below), equivalent,
                     but shorter 60 aa, to Q49988|PPGK_MYCLE|ML1023|U1764FG
                     POLYPHOSPHATE GLUCOKINASE from Mycobacterium leprae (324
                     aa), FASTA scores: opt: 1411, E(): 5.6e-80, (82.8%
                     identity in 262 aa overlap). Also highly similar (or just
                     similar) to others e.g. Q9ADE8|PPGK from Streptomyces
                     coelicolor (246 aa), FASTA scores: opt: 912, E(): 3e-49,
                     (57.3% identity in 239 aa overlap); Q9AGV8|PPGK from
                     Corynebacterium ammoniagenes (Brevibacterium ammoniagenes)
                     (277 aa), FASTA scores: opt: 890, E(): 7.5e-48, (57.75%
                     identity in 239 aa overlap); P40184|GLK_STRCO|SC6E10.20c
                     from Streptomyces coelicolor (317 aa), FASTA scores: opt:
                     233, E(): 3.2e-07, (31.3% identity in 163 aa overlap);
                     etc. Protein product from Mb2721 detected using shotgun
                     mass spectrometry and SWATH mass spectrometry. Mb2721
                     found to be expressed during exponential growth in
                     Sauton's minimal media by RNA-sequencing (TPM > 20)."
                     /db_xref="GOA:A0A1R3Y1Y1"
                     /db_xref="InterPro:IPR000600"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3Y1Y1"
BEGIN
        1 MTSTGPETSE TPGATTQRHG FGIDVGGSGI KGGIVDLDTG QLIGDRIKLL TPQPATPLAV
       61 AKTIAEVVNG FGWRGPLGVT YPGVVTHGVV RTAANVDKSW IGTNARDTIG AELGGQQVTI
      121 LNDADAAGLA ETRYGAGKNN PGLVVLLTFG TGIGSAVIHN GTLIPNTEFG HLEVGGKEAE
      181 ERAASSVKEK NDWTYPKWAK QVTRVLIAIE NAIWPDLFIA GGGISRKADK WVPLLENRTP
      241 VVPAALQNTA GIVGAAMASV ADTTH
//