LOCUS SIU01339.1 265 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POLYPHOSPHATE GLUCOKINASE PPGK (POLYPHOSPHATE-GLUCOSE PHOSPHOTRANSFERASE) protein. ACCESSION LT708304-2753 PROTEIN_ID SIU01339.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="ppgK" /locus_tag="BQ2027_MB2721" /note="Mb2721, ppgK, len: 265 aa. Equivalent to Rv2702, len: 265 aa, from Mycobacterium tuberculosis strain H37Rv, (99.6% identity in 265 aa overlap). ppgK, polyphosphate glucokinase (EC 2.7.1.2) (see citation below), equivalent, but shorter 60 aa, to Q49988|PPGK_MYCLE|ML1023|U1764FG POLYPHOSPHATE GLUCOKINASE from Mycobacterium leprae (324 aa), FASTA scores: opt: 1411, E(): 5.6e-80, (82.8% identity in 262 aa overlap). Also highly similar (or just similar) to others e.g. Q9ADE8|PPGK from Streptomyces coelicolor (246 aa), FASTA scores: opt: 912, E(): 3e-49, (57.3% identity in 239 aa overlap); Q9AGV8|PPGK from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (277 aa), FASTA scores: opt: 890, E(): 7.5e-48, (57.75% identity in 239 aa overlap); P40184|GLK_STRCO|SC6E10.20c from Streptomyces coelicolor (317 aa), FASTA scores: opt: 233, E(): 3.2e-07, (31.3% identity in 163 aa overlap); etc. Protein product from Mb2721 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2721 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:A0A1R3Y1Y1" /db_xref="InterPro:IPR000600" /db_xref="UniProtKB/TrEMBL:A0A1R3Y1Y1" BEGIN 1 MTSTGPETSE TPGATTQRHG FGIDVGGSGI KGGIVDLDTG QLIGDRIKLL TPQPATPLAV 61 AKTIAEVVNG FGWRGPLGVT YPGVVTHGVV RTAANVDKSW IGTNARDTIG AELGGQQVTI 121 LNDADAAGLA ETRYGAGKNN PGLVVLLTFG TGIGSAVIHN GTLIPNTEFG HLEVGGKEAE 181 ERAASSVKEK NDWTYPKWAK QVTRVLIAIE NAIWPDLFIA GGGISRKADK WVPLLENRTP 241 VVPAALQNTA GIVGAAMASV ADTTH //