LOCUS SIU01334.1 154 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable deoxyuridine 5'-triphosphate nucleotidohydrolase dut ( dutpase) (dutp pyrophosphatase) (deoxyuridine 5'-triphosphatase) (dutp diphosphatase) (deoxyuridine-triphosphatase) protein. ACCESSION LT708304-2748 PROTEIN_ID SIU01334.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="dut" /locus_tag="BQ2027_MB2716C" /note="Mb2716c, dut, len: 154 aa. Equivalent to Rv2697c, len: 154 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 154 aa overlap). Probable dut, deoxyuridine 5'-triphosphate nucleotidohydrolase (EC 3.6.1.23), equivalent to Q49992|DUT_MYCLE|ML1028 DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE from Mycobacterium leprae (154 aa), FASTA scores: opt: 928, E(): 2.1e-51, (90.25% identity in 154 aa overlap). Also highly similar to others e.g. O54134|DUT_STRCO|SC2E9.09 from Streptomyces coelicolor (183 aa), FASTA scores: opt: 534, E(): 1.2e-26, (56.1% identity in 148 aa overlap); O66592|DUT_AQUAE|AQ_220 from Aquifex aeolicus (150 aa), FASTA scores: opt: 398, E(): 3.3e-18, (48.05% identity in 152 aa overlap); Q9X3X5|DUT_ZYMMO from Zymomonas mobilis (146 aa), FASTA scores: opt: 396, E(): 4.4e-18, (49.0% identity in 147 aa overlap); etc. BELONGS TO THE DUTPASE FAMILY. Protein product from Mb2716c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2716c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P0A553" /db_xref="InterPro:IPR008181" /db_xref="InterPro:IPR029054" /db_xref="InterPro:IPR033704" /db_xref="InterPro:IPR036157" /db_xref="UniProtKB/Swiss-Prot:P0A553" BEGIN 1 MSTTLAIVRL DPGLPLPSRA HDGDAGVDLY SAEDVELAPG RRALVRTGVA VAVPFGMVGL 61 VHPRSGLATR VGLSIVNSPG TIDAGYRGEI KVALINLDPA APIVVHRGDR IAQLLVQRVE 121 LVELVEVSSF DEAGLASTSR GDGGHGSSGG HASL //