LOCUS SIU01326.1 405 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 conserved alanine and valine and glycine rich protein protein. ACCESSION LT708304-2740 PROTEIN_ID SIU01326.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB2708C" /note="Mb2708c, -, len: 405 aa. Equivalent to Rv2689c, len: 405 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 405 aa overlap). Conserved hypothetical ala-, val-, gly-rich protein, similar to O54099|SC10A5.06 HYPOTHETICAL 49.5 KDA PROTEIN from Streptomyces coelicolor (458 aa), FASTA scores: opt: 455, E(): 2.7e-20, (38.35% identity in 417 aa overlap); and shows weak similarity in part with several methyltransferases (EC 2.1.1.-) e.g. Q9X0H9|TM1094 PUTATIVE RNA METHYLTRANSFERASE from Thermotoga maritima (439 aa), FASTA scores: opt: 306, E(): 3e-11, (25.9% identity in 436 aa overlap); AK79403|CAC1435 S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASES from Clostridium acetobutylicum (456 aa), FASTA scores: opt: 294, E(): 1.6e-10, (23.4% identity in 449 aa overlap); Q9A8M7|CC1326 RNA METHYLTRANSFERASE from Caulobacter crescentus (415 aa), FASTA scores: opt: 247, E(): 1.1e-07, (28.4% identity in 433 aa overlap); etc. Equivalent to AAK47078 from Mycobacterium tuberculosis strain CDC1551 (434 aa) but shorter 29 aa. Other less probable starts possible. Protein product from Mb2708c detected using SWATH mass spectrometry. Mb2708c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y2Q7" /db_xref="InterPro:IPR002792" /db_xref="InterPro:IPR010280" /db_xref="InterPro:IPR012340" /db_xref="InterPro:IPR029063" /db_xref="UniProtKB/TrEMBL:A0A1R3Y2Q7" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MTRAGDDAVN LTLVTGAPAN GGSCVAHHEG RVVFVRYALP GERVRARVTA QRGSYWHAEA 61 FEVIDPSPDR IGSLCSIAGA DGAGCCDLAF AAPEAARTLK AQVVANQLER LGRHSWQGEA 121 QPLSDAGPTG WRIRVRLDVG ADRRPGFHRY HSGELVTDLD CGQLPVGMLD GLVAADWPPE 181 AQLYVALDDD GERHVVCSVR QGPRNRTRTV TNVVEGAYHA HQRVHRRSWR VPVTAFWQAH 241 RDAAAVYSDL IADWAQPAPG MTAWDLYGGA GVFAAVLGEA VGESGRVLTV DTSRLASGAA 301 RAALVDLPQV EVVTGSVRRV LAVQPAGADL AVLDPPRSGA GREVVDLLAG AGVPRLIHIG 361 CEAASFARDI GLYRGHGYAV EKIKVFDAFP LTHYVECVAL LTRKV //