LOCUS SIU01308.1 258 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE BIFUNCTIONAL ENZYME RIBOFLAVIN BIOSYNTHESIS PROTEIN RIBD: DIAMINOHYDROXYPHOSPHORIBOSYLAMINOPYRIMIDINE DEAMINASE (RIBOFLAVIN-SPECIFIC DEAMINASE) + 5-AMINO-6-(5-PHOSPHORIBOSYLAMINO) URACIL REDUCTASE (HTP REDUCTASE) protein. ACCESSION LT708304-2722 PROTEIN_ID SIU01308.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="ribD" /locus_tag="BQ2027_MB2690" /note="Mb2690, ribD, len: 258 aa. Equivalent to Rv2671, len: 258 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 258 aa overlap). Possible ribD (alternate gene name: ribG), bifunctional riboflavin biosynthesis protein incuding diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5-amino-6-(5-phosphoribosylamino) uracil reductase (EC 3.5.4.26 and 1.1.1.193), highly similar to O05684|MLC1351.23|ML1340 POSSIBLE REDUCTASE from Mycobacterium leprae (268 aa), FASTA scores: opt: 1211, E(): 3e-68, (72.9% identity in 251 aa overlap). Also weakly similar to others e.g. Q9HWX2|RIBD|PA4056 RIBOFLAVIN-SPECIFIC DEAMINASE/REDUCTASE from Pseudomonas aeruginosa (373 aa), FASTA scores: opt: 211, E(): 6.3e-06, (30.1% identity in 216 aa overlap); Q9HQA1|RIBG|VNG1256G RIBOFLAVIN-SPECIFIC DEAMINASE from Halobacterium sp. strain NRC-1 (220 aa), FASTA scores: opt: 202, E(): 1.5e-05, (27.0% identity in 174 aa overlap); O28272|RIB7_ARCFU|AF2007 PUTATIVE 5-AMINO-6-(5-PHOSPHORIBOSYLAMINO)URACIL REDUCTASE (HTP REDUCTASE) (EC 1.1.1.193) from Archaeoglobus fulgidus (219 aa), FASTA scores: opt: 209, E(): 5.4e-06, (24.15% identity in 211 aa overlap); P25539|RIBD_ECOLI|RIBG|B0414 from Escherichia coli strain K12 (367 aa), FASTA scores: opt: 185, E(): 0.00026, (26.7% identity in 221 aa overlap); etc. But also similar to several hydrolases e.g. Q9X825|SC9B1.05 PUTATIVE HYDROLASE from Streptomyces coelicolor (265 aa), FASTA scores: opt: 536, E(): 2.9e-26, (44.25% identity in 235 aa overlap); Q9RKM1|SCD17.10 PUTATIVE BIFUNCTIONAL ENZYME DEAMINASE/REDUCTASE from Streptomyces coelicolor (376 aa), FASTA scores: opt: 228, E(): 5.6e-07, (33.5% identity in 188 aa overlap); etc. Equivalent to AAK47060 from Mycobacterium tuberculosis strain CDC1551 (239 aa) but longer 19 aa. SUPPOSED BELONG TO THE CYTIDINE AND DEOXYCYTIDYLATE DEAMINASES FAMILY IN THE N-TERMINAL SECTION; and TO THE HTP REDUCTASE FAMILY IN THE C-TERMINAL SECTION. Protein product from Mb2690 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2690 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y294" /db_xref="InterPro:IPR002734" /db_xref="InterPro:IPR024072" /db_xref="UniProtKB/TrEMBL:A0A1R3Y294" BEGIN 1 MPDSGQLGAA DTPLRLLSSV HYLTDGELPQ LYDYPDDGTW LRANFISSLD GGATVDGTSG 61 AMAGPGDRFV FNLLRELADV IVVGVGTVRI EGYSGVRMGV VQRQHRQARG QSEVPQLAIV 121 TRSGRLDRDM AVFTRTEMAP LVLTTTAVAD DTRQRLAGLA EVIACSGDDP GTVDEAVLVS 181 QLAARGLRRI LTEGGPTLLG TFVERDVLDE LCLTIAPYVV GGLARRIVTG PGQVLTRMRC 241 AHVLTDDSGY LYTRYVKT //