LOCUS       SIU01308.1               258 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE
            BIFUNCTIONAL ENZYME RIBOFLAVIN BIOSYNTHESIS PROTEIN RIBD:
            DIAMINOHYDROXYPHOSPHORIBOSYLAMINOPYRIMIDINE DEAMINASE
            (RIBOFLAVIN-SPECIFIC DEAMINASE) + 5-AMINO-6-(5-PHOSPHORIBOSYLAMINO)
            URACIL REDUCTASE (HTP REDUCTASE) protein.
ACCESSION   LT708304-2722
PROTEIN_ID  SIU01308.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="ribD"
                     /locus_tag="BQ2027_MB2690"
                     /note="Mb2690, ribD, len: 258 aa. Equivalent to Rv2671,
                     len: 258 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 258 aa overlap). Possible ribD
                     (alternate gene name: ribG), bifunctional riboflavin
                     biosynthesis protein incuding
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase and
                     5-amino-6-(5-phosphoribosylamino) uracil reductase (EC
                     3.5.4.26 and 1.1.1.193), highly similar to
                     O05684|MLC1351.23|ML1340 POSSIBLE REDUCTASE from
                     Mycobacterium leprae (268 aa), FASTA scores: opt: 1211,
                     E(): 3e-68, (72.9% identity in 251 aa overlap). Also
                     weakly similar to others e.g. Q9HWX2|RIBD|PA4056
                     RIBOFLAVIN-SPECIFIC DEAMINASE/REDUCTASE from Pseudomonas
                     aeruginosa (373 aa), FASTA scores: opt: 211, E(): 6.3e-06,
                     (30.1% identity in 216 aa overlap); Q9HQA1|RIBG|VNG1256G
                     RIBOFLAVIN-SPECIFIC DEAMINASE from Halobacterium sp.
                     strain NRC-1 (220 aa), FASTA scores: opt: 202, E():
                     1.5e-05, (27.0% identity in 174 aa overlap);
                     O28272|RIB7_ARCFU|AF2007 PUTATIVE
                     5-AMINO-6-(5-PHOSPHORIBOSYLAMINO)URACIL REDUCTASE (HTP
                     REDUCTASE) (EC 1.1.1.193) from Archaeoglobus fulgidus (219
                     aa), FASTA scores: opt: 209, E(): 5.4e-06, (24.15%
                     identity in 211 aa overlap); P25539|RIBD_ECOLI|RIBG|B0414
                     from Escherichia coli strain K12 (367 aa), FASTA scores:
                     opt: 185, E(): 0.00026, (26.7% identity in 221 aa
                     overlap); etc. But also similar to several hydrolases e.g.
                     Q9X825|SC9B1.05 PUTATIVE HYDROLASE from Streptomyces
                     coelicolor (265 aa), FASTA scores: opt: 536, E(): 2.9e-26,
                     (44.25% identity in 235 aa overlap); Q9RKM1|SCD17.10
                     PUTATIVE BIFUNCTIONAL ENZYME DEAMINASE/REDUCTASE from
                     Streptomyces coelicolor (376 aa), FASTA scores: opt: 228,
                     E(): 5.6e-07, (33.5% identity in 188 aa overlap); etc.
                     Equivalent to AAK47060 from Mycobacterium tuberculosis
                     strain CDC1551 (239 aa) but longer 19 aa. SUPPOSED BELONG
                     TO THE CYTIDINE AND DEOXYCYTIDYLATE DEAMINASES FAMILY IN
                     THE N-TERMINAL SECTION; and TO THE HTP REDUCTASE FAMILY IN
                     THE C-TERMINAL SECTION. Protein product from Mb2690
                     detected using shotgun mass spectrometry and SWATH mass
                     spectrometry. Mb2690 found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:A0A1R3Y294"
                     /db_xref="InterPro:IPR002734"
                     /db_xref="InterPro:IPR024072"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3Y294"
BEGIN
        1 MPDSGQLGAA DTPLRLLSSV HYLTDGELPQ LYDYPDDGTW LRANFISSLD GGATVDGTSG
       61 AMAGPGDRFV FNLLRELADV IVVGVGTVRI EGYSGVRMGV VQRQHRQARG QSEVPQLAIV
      121 TRSGRLDRDM AVFTRTEMAP LVLTTTAVAD DTRQRLAGLA EVIACSGDDP GTVDEAVLVS
      181 QLAARGLRRI LTEGGPTLLG TFVERDVLDE LCLTIAPYVV GGLARRIVTG PGQVLTRMRC
      241 AHVLTDDSGY LYTRYVKT
//