LOCUS SIU01266.1 464 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 pe- pgrs family protein pe_pgrs45 protein. ACCESSION LT708304-2680 PROTEIN_ID SIU01266.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="PE_PGRS45" /locus_tag="BQ2027_MB2648C" /note="Mb2648c, PE_PGRS45, len: 464 aa. Equivalent to Rv2615c, len: 461 aa, from Mycobacterium tuberculosis strain H37Rv, (98.7% identity in 464 aa overlap). Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins, highly similar to many e.g. P71664|Rv1396c|MTCY21B4.13c from Mycobacterium tuberculosis (576 aa), FASTA scores: opt: 1629, E(): 4.8e-58, (56.65% identity in 482 aa overlap). Equivalent to AAK47006 from Mycobacterium tuberculosis strain CDC1551 (476 aa) but shorter 15 aa. REMARK-M.bovis-M.tuberculosis: In Mycobacterium bovis, a 9 bp in-frame insertion (*-ccgccgttt) leads to a slightly longer product compared to its homolog in Mycobacterium tuberculosis strain H37Rv (464 aa versus 461 aa). Mb2648c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="InterPro:IPR000084" /db_xref="UniProtKB/TrEMBL:A0A1R3Y2J2" BEGIN 1 MSFVNVAPQL VSTAAADAAR IGSAINTANT AAAATTQVLV AAQDEVSTAI AALFGSHGQH 61 YQAISAQVAA YQERFVLALS QAGSTYAVAE AASATPLQNV LDAINAPVQS LTGRPLIGDG 121 ANGIDGTGQA GGNGGWLWGN GGNSGSGAPG QAGGAGGAAG LIGNGGAGGA GGQGLPFEAG 181 ANGGAGGAGG WLFGNGGAGG NGGIGGAGTN LAIGGHGGNG GNAGLIGAGG TGGAGGTGGG 241 EPSAGASGGN GGNGGNGGLL IGNSGDGGAA GNGAGISQNG PASGFGGNGG HAGTTGLIGN 301 GGNGGAGGAG GDVSADFGGV GFGGQGGNGG AGGLLYGNGG AGGNGGAAGS PGSVTAFGGN 361 GGSGGSGGNG GNALIGNAGA GGSAGAGGNG ASAGTAGGSG GDGGKGGNGG SVGLIGNGGN 421 GGNGGNGGAG SLFNGAPGFG GPGGSGGASL LGPPGLAGTN GADG //