LOCUS SIU01243.1 188 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE CROSSOVER JUNCTION ENDODEOXYRIBONUCLEASE RUVC (HOLLIDAY JUNCTION NUCLEASE) (HOLLIDAY JUNCTION RESOLVASE) protein. ACCESSION LT708304-2657 PROTEIN_ID SIU01243.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="ruvC" /locus_tag="BQ2027_MB2625C" /note="Mb2625c, ruvC, len: 188 aa. Equivalent to Rv2594c, len: 188 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 188 aa overlap). Probable ruvC, Holliday junction resolvase (EC 3.1.22.4) (see citations below), equivalent to P40834|RUVC_MYCLE|ML0481|B1177_C3_226 CROSSOVER JUNCTION ENDODEOXYRIBONUCLEASE from Mycobacterium leprae (188 aa), FASTA scores: opt: 984, E(): 2.3e-55, (81.0% identity in 184 aa overlap). Also highly similar to others e.g. Q9AE11|RUVC from Corynebacterium glutamicum (Brevibacterium flavum) (221 aa), FASTA scores: opt: 713, E(): 3.6e-38, (56.9% identity in 188 aa overlap); Q9L289|RUVC_STRCO|SCL2.10c from Streptomyces coelicolor (188 aa), FASTA scores: opt: 704, E(): 1.2e-37, (60.65% identity in 178 aa overlap); P24239|RUVC_ECOLI|B1863 from Escherichia coli strain K12 (172 aa), FASTA scores: opt: 322, E(): 1.6e-13, (38.65% identity in 163 aa overlap); etc. BELONGS TO THE RUVC FAMILY. COFACTOR: MAGNESIUM. Mb2625c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P66761" /db_xref="InterPro:IPR002176" /db_xref="InterPro:IPR012337" /db_xref="InterPro:IPR020563" /db_xref="InterPro:IPR036397" /db_xref="UniProtKB/Swiss-Prot:P66761" BEGIN 1 MRVMGVDPGL TRCGLSLIES GRGRQLTALD VDVVRTPSDA ALAQRLLAIS DAVEHWLDTH 61 HPEVVAIERV FSQLNVTTVM GTAQAGGVIA LAAAKRGVDV HFHTPSEVKA AVTGNGSADK 121 AQVTAMVTKI LALQAKPTPA DAADALALAI CHCWRAPTIA RMAEATSRAE ARAAQQRHAY 181 LAKLKAAR //