LOCUS SIU01239.1 1168 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
FATTY-ACID-CoA LIGASE FADD9 (FATTY-ACID-COA SYNTHETASE)
(FATTY-ACID-COA SYNTHASE) protein.
ACCESSION LT708304-2653
PROTEIN_ID SIU01239.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/gene="fadD9"
/locus_tag="BQ2027_MB2621"
/note="Mb2621, fadD9, len: 1168 aa. Equivalent to Rv2590,
len: 1168 aa, from Mycobacterium tuberculosis strain
H37Rv, (99.8% identity in 1168 aa overlap). Probable
fadD9, fatty-acid-CoA synthetase (EC 6.2.1.-), highly
similar to O69484|FADD9 (alias Q9CCT4|FADD9|ML0484 but
longer 14 aa) PUTATIVE ACYL-COA SYNTHETASE from
Mycobacterium leprae (1174 aa), FASTA scores: opt: 5247,
E(): 0, (68.0% identity in 1178 aa overlap);
Q49651|LCLA|B1177_F1_23 PUTATIVE
LONG-CHAIN-FATTY-ACID--COA LIGASE from Mycobacterium
leprae (827 aa), FASTA scores: opt: 3170, E(): 7.1e-181,
(63.9% identity in 770 aa overlap). N-terminal (700
residues) similar to other long chain fatty acid ligases.
And C-terminus highly similar to C-terminus of Q9XCF2|PSTB
PSTB PROTEIN from Mycobacterium avium (2552 aa), FASTA
scores: opt: 2083, E(): 8.4e-116, (40.8% identity in 1150
aa overlap) (and weak similarity on N-terminus);
Q49653|POL1|B1177_F2_70 POL1 PROTEIN from Mycobacterium
leprae (400 aa), FASTA scores: opt: 2066, E(): 2e-115,
(76.25% identity in 404 aa overlap). C-terminal part
highly similar to polyketide synthases and peptides
synthases (weak similarity on N-terminus) e.g.
Q10896|Rv0101|MTCY251.20|NRP PROBABLE PEPTIDE SYNTHETASE
from Mycobacterium tuberculosis (2512 aa), FASTA scores:
opt: 1988, E(): 3.7e-110, (40.2% identity in 1181 aa
overlap); etc. Contains PS00455 putative AMP-binding
domain signature, and PS00061 Short-chain alcohol
dehydrogenase family signature. SEEMS TO BELONG TO THE
ATP-DEPENDENT AMP-BINDING ENZYME FAMILY, AND TO THE
SHORT-CHAIN DEHYDROGENASES/REDUCTASES (SDR) FAMILY.
Protein product from Mb2621 detected using shotgun mass
spectrometry and SWATH mass spectrometry. Mb2621 found to
be expressed during exponential growth in Sauton's minimal
media by RNA-sequencing (TPM > 20)."
/db_xref="GOA:A0A1R3Y1N0"
/db_xref="InterPro:IPR000873"
/db_xref="InterPro:IPR009081"
/db_xref="InterPro:IPR010080"
/db_xref="InterPro:IPR013120"
/db_xref="InterPro:IPR020806"
/db_xref="InterPro:IPR020845"
/db_xref="InterPro:IPR036291"
/db_xref="InterPro:IPR036736"
/db_xref="InterPro:IPR042099"
/db_xref="UniProtKB/TrEMBL:A0A1R3Y1N0"
/experiment="experimental evidence, no additional details
recorded"
BEGIN
1 MSINDQRLTR RVEDLYASDA QFAAASPNEA ITQAIDQPGV ALPQLIRMVM EGYADRPALG
61 QRALRFVTDP DSGRTMVELL PRFETITYRE LWARAGTLAT ALSAEPAIRP GDRVCVLGFN
121 SVDYTTIDIA LIRLGAVSVP LQTSAPVTGL RPIVTETEPT MIATSIDNLG DAVEVLAGHA
181 PARLVVFDYH GKVDTHREAV EAARARLAGS VTIDTLAELI ERGRALPATP IADSADDALA
241 LLIYTSGSTG APKGAMYRES QVMSFWRKSS GWFEPSGYPS ITLNFMPMSH VGGRQVLYGT
301 LSNGGTAYYV AKSDLSTLFE DLALVRPTEL CFVPRIWDMV FAEFHSEVDR RLVDGADRAA
361 LEAQVKAELR ENVLGGRFVM ALTGSAPISA EMTAWVESLL ADVHLVEGYG STEAGMVLND
421 GMVRRPAVID YKLVDVPELG YFGTDQPYPR GELLVKTQTM FPGYYQRPDV TAEVFDPDGF
481 YRTGDIMAKV GPDQFVYLDR RNNVLKLSQG EFIAVSKLEA VFGDSPLVRQ IFIYGNSARA
541 YPLAVVVPSG DALSRHGIEN LKPVISESLQ EVARAAGLQS YEIPRDFIIE TTPFTLENGL
601 LTGIRKLARP QLKKFYGERL ERLYTELADS QSNELRELRQ SGPDAPVLPT LCRAAAALLG
661 STAADVRPDA HFADLGGDSL SALSLANLLH EIFGVDVPVG VIVSPASDLR ALADHIEAAR
721 TGVRRPSFAS IHGRSATEVH ASDLTLDKFI DAATLAAAPN LPAPSAQVRT VLLTGATGFL
781 GRYLALEWLD RMDLVNGKLI CLVRARSDEE AQARLDATFD SGDPYLVRHY RELGAGRLEV
841 LAGDKGEADL GLDRVTWQRL ADTVDLIVDP AALVNHVLPY SQLFGPNAAG TAELLRLALT
901 GKRKPYIYTS TIAVGEQIPP EAFTEDADIR AISPTRRIDD SYANGYANSK WAGEVLLREA
961 HEQCGLPVTV FRCDMILADT SYTGQLNLPD MFTRLMLSLA ATGIAPGSFY ELDAHGNRQR
1021 AHYDGLPVEF VAEAICTLGT HSPDRFVTYH VMNPYDDGIG LDEFVDWLNS PTSGSGCTIQ
1081 RIADYGEWLQ RFETSLRALP DRQRHTSLLP LLHNYREPAK PICGSIAPTD QFRAAVQEAK
1141 IGPDKDIPHL TAAIIAKYIS NLRLLGLL
//