LOCUS SIU01232.1 790 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE GTP PYROPHOSPHOKINASE RELA (ATP:GTP 3'-PYROPHOSPHOTRANSFERASE) (PPGPP SYNTHETASE I) ((P)PPGPP SYNTHETASE) (GTP DIPHOSPHOKINASE) protein. ACCESSION LT708304-2646 PROTEIN_ID SIU01232.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="relA" /locus_tag="BQ2027_MB2614C" /note="Mb2614c, relA, len: 790 aa. Equivalent to Rv2583c, len: 790 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 790 aa overlap). Probable relA, GTP pyrophosphokinase (EC 2.7.6.5), equivalent to Q49640|RELA_MYCLE|ML0491|MLCB1259.09|B1177_C1_168 PROBABLE GTP PYROPHOSPHOKINASE from Mycobacterium leprae (787 aa), FASTA scores: opt: 4834, E(): 0, (93.4% identity in 790 aa overlap). Also highly similar to others e.g. O87331|RELA_CORGL|RELA|REL from Corynebacterium glutamicum (Brevibacterium flavum) (760 aa), FASTA scores: opt: 3375, E(): 1.6e-196, (67.0% identity in 758 aa overlap); O85709|RELA_STRAT from Streptomyces antibioticus (841 aa), FASTA scores: opt: 3209, E(): 1.9e-186, (63.85% identity in 786 aa overlap); Q9KDH1|RELA|BH1242 from Bacillus halodurans (728 aa), FASTA scores: opt: 2195,E(): 3.8e-125, (45.65% identity in 714 aa overlap); etc. BELONGS TO THE RELA / SPOT FAMILY. Protein product from Mb2614c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2614c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P66015" /db_xref="InterPro:IPR002912" /db_xref="InterPro:IPR003607" /db_xref="InterPro:IPR004095" /db_xref="InterPro:IPR004811" /db_xref="InterPro:IPR007685" /db_xref="InterPro:IPR012675" /db_xref="InterPro:IPR012676" /db_xref="InterPro:IPR033655" /db_xref="UniProtKB/Swiss-Prot:P66015" BEGIN 1 MAEDQLTAQA VAPPTEASAA LEPALETPES PVETLKTSIS ASRRVRARLA RRMTAQRSTT 61 NPVLEPLVAV HREIYPKADL SILQRAYEVA DQRHASQLRQ SGDPYITHPL AVANILAELG 121 MDTTTLVAAL LHDTVEDTGY TLEALTEEFG EEVGHLVDGV TKLDRVVLGS AAEGETIRKM 181 ITAMARDPRV LVIKVADRLH NMRTMRFLPP EKQARKARET LEVIAPLAHR LGMASVKWEL 241 EDLSFAILHP KKYEEIVRLV AGRAPSRDTY LAKVRAEIVN TLTASKIKAT VEGRPKHYWS 301 IYQKMIVKGR DFDDIHDLVG VRILCDEIRD CYAAVGVVHS LWQPMAGRFK DYIAQPRYGV 361 YQSLHTTVVG PEGKPLEVQI RTRDMHRTAE YGIAAHWRYK EAKGRNGVLH PHAAAEIDDM 421 AWMRQLLDWQ REAADPGEFL ESLRYDLAVQ EIFVFTPKGD VITLPTGSTP VDFAYAVHTE 481 VGHRCIGARV NGRLVALERK LENGEVVEVF TSKAPNAGPS RDWQQFVVSP RAKTKIRQWF 541 AKERREEALE TGKDAMAREV RRGGLPLQRL VNGESMAAVA RELHYADVSA LYTAIGEGHV 601 SAKHVVQRLL AELGGIDQAE EELAERSTPA TMPRRPRSTD DVGVSVPGAP GVLTKLAKCC 661 TPVPGDVIMG FVTRGGGVSV HRTDCTNAAS LQQQAERIIE VLWAPSPSSV FLVAIQVEAL 721 DRHRLLSDVT RALADEKVNI LSASVTTSGD RVAISRFTFE MGDPKHLGHL LNAVRNVEGV 781 YDVYRVTSAA //