LOCUS SIU01228.1 300 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE HALOALKANE DEHALOGENASE DHAA (1-CHLOROHEXANE HALIDOHYDROLASE) protein. ACCESSION LT708304-2642 PROTEIN_ID SIU01228.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="dhaA" /locus_tag="BQ2027_MB2610" /note="Mb2610, dhaA, len: 300 aa. Equivalent to Rv2579, len: 300 aa, from Mycobacterium tuberculosis strain H37Rv, (99.3% identity in 300 aa overlap). Possible dhaA, haloalkane dehalogenase (EC 3.8.1.5), strictly equivalent to Q9XB14|ISO-RV2579 HALOALKANE DEHALOGENASE (1-chlorohexane halidohydrolase) (EC 3.8.1.5) from Mycobacterium bovis (300 aa), FASTA scores: opt: 2075, E(): 7.1e-125, (99.35% identity in 300 aa overlap); note that only two residues, 120 and 293 are different. Also highly similar to others e.g. Q9ZER0|DHAAF HALOALKANE DEHALOGENASE from Mycobacterium sp strain GP1 (307 aa), FASTA scores: opt: 842, E(): 2.3e-46, (44.95% identity in 298 aa overlap); Q53042|DHAA HALOALKANE DEHALOGENASE from Rhodococcus rhodochrous, and Pseudomonas pavonaceae (293 aa), FASTA scores: opt: 837, E(): 4.5e-46, (44.6% identity in 298 aa overlap); etc. Note that this protein may also be a 1,3,4,6-tetrachloro-1,4-cyclohexadiene hydrolase (EC 3.8.1.-), because also highly similar to P51698|LINB_PSEPA 1,3,4,6-TETRACHLORO-1,4-CYCLOHEXADIENE HYDROLASE from Pseudomonas paucimobilis (Sphingomonas paucimobilis) (see first citation below) (296 aa), FASTA scores: opt: 1494, E(): 6.8e-88, (69.5% identity in 295 aa overlap). Also shows some similarity with proteins from Mycobacterium tuberculosis e.g. Q50670|YM96_MYCTU|Rv2296|MT2353|MTCY339. 14c PUTATIVE HALOALKANE DEHALOGENASE (300 aa), FASTA scores: opt: 302, E(): 5.3e-12, (30.85% identity in 295 aa overlap); and Q50600|YJ33_MYCTU|Rv1833c|MT1881|MTCY1A11.10 HYPOTHETICAL 32.2 KDA PROTEIN (286 aa), FASTA scores: opt: 286, E(): 5.3e-11, (29.85% identity in 288 aa overlap). MAY BE BELONG TO ALPHA/BETA HYDROLASE FOLD FAMILY. Note that previously known as linB. Protein product from Mb2610 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2610 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:Q9XB14" /db_xref="InterPro:IPR000073" /db_xref="InterPro:IPR000639" /db_xref="InterPro:IPR023594" /db_xref="InterPro:IPR029058" /db_xref="UniProtKB/Swiss-Prot:Q9XB14" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MTAFGVEPYG QPKYLEIAGK RMAYIDEGKG DAIVFQHGNP TSSYLWRNIM PHLEGLGRLV 61 ACDLIGMGAS DKLSPSGPDR YSYGEQRDFL FALWDTLDLG DHVVLVLHDW GSALGFDWAN 121 QHRDRVQGIA FMEAIVTPMT WADWPPAVRG VFQGFRSPQG EPMALEHNIF VERVLPGAIL 181 RQLSDEEMNH YRRPFVNGGE DRRPTLSWPR NLPIDGEPAE VVALVNEYRS WLEETDMPKL 241 FINAEPGAII TGRIRDYVRS WPNQTEITVP GVHFVQEDSP EEIGAAIAQF VRQLRSAAGV //