LOCUS       SIU01228.1               300 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE
            HALOALKANE DEHALOGENASE DHAA (1-CHLOROHEXANE HALIDOHYDROLASE)
            protein.
ACCESSION   LT708304-2642
PROTEIN_ID  SIU01228.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="dhaA"
                     /locus_tag="BQ2027_MB2610"
                     /note="Mb2610, dhaA, len: 300 aa. Equivalent to Rv2579,
                     len: 300 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (99.3% identity in 300 aa overlap). Possible dhaA,
                     haloalkane dehalogenase (EC 3.8.1.5), strictly equivalent
                     to Q9XB14|ISO-RV2579 HALOALKANE DEHALOGENASE
                     (1-chlorohexane halidohydrolase) (EC 3.8.1.5) from
                     Mycobacterium bovis (300 aa), FASTA scores: opt: 2075,
                     E(): 7.1e-125, (99.35% identity in 300 aa overlap); note
                     that only two residues, 120 and 293 are different. Also
                     highly similar to others e.g. Q9ZER0|DHAAF HALOALKANE
                     DEHALOGENASE from Mycobacterium sp strain GP1 (307 aa),
                     FASTA scores: opt: 842, E(): 2.3e-46, (44.95% identity in
                     298 aa overlap); Q53042|DHAA HALOALKANE DEHALOGENASE from
                     Rhodococcus rhodochrous, and Pseudomonas pavonaceae (293
                     aa), FASTA scores: opt: 837, E(): 4.5e-46, (44.6% identity
                     in 298 aa overlap); etc. Note that this protein may also
                     be a 1,3,4,6-tetrachloro-1,4-cyclohexadiene hydrolase (EC
                     3.8.1.-), because also highly similar to P51698|LINB_PSEPA
                     1,3,4,6-TETRACHLORO-1,4-CYCLOHEXADIENE HYDROLASE from
                     Pseudomonas paucimobilis (Sphingomonas paucimobilis) (see
                     first citation below) (296 aa), FASTA scores: opt: 1494,
                     E(): 6.8e-88, (69.5% identity in 295 aa overlap). Also
                     shows some similarity with proteins from Mycobacterium
                     tuberculosis e.g. Q50670|YM96_MYCTU|Rv2296|MT2353|MTCY339.
                     14c PUTATIVE HALOALKANE DEHALOGENASE (300 aa), FASTA
                     scores: opt: 302, E(): 5.3e-12, (30.85% identity in 295 aa
                     overlap); and Q50600|YJ33_MYCTU|Rv1833c|MT1881|MTCY1A11.10
                     HYPOTHETICAL 32.2 KDA PROTEIN (286 aa), FASTA scores: opt:
                     286, E(): 5.3e-11, (29.85% identity in 288 aa overlap).
                     MAY BE BELONG TO ALPHA/BETA HYDROLASE FOLD FAMILY. Note
                     that previously known as linB. Protein product from Mb2610
                     detected using shotgun mass spectrometry and SWATH mass
                     spectrometry. Mb2610 found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:Q9XB14"
                     /db_xref="InterPro:IPR000073"
                     /db_xref="InterPro:IPR000639"
                     /db_xref="InterPro:IPR023594"
                     /db_xref="InterPro:IPR029058"
                     /db_xref="UniProtKB/Swiss-Prot:Q9XB14"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MTAFGVEPYG QPKYLEIAGK RMAYIDEGKG DAIVFQHGNP TSSYLWRNIM PHLEGLGRLV
       61 ACDLIGMGAS DKLSPSGPDR YSYGEQRDFL FALWDTLDLG DHVVLVLHDW GSALGFDWAN
      121 QHRDRVQGIA FMEAIVTPMT WADWPPAVRG VFQGFRSPQG EPMALEHNIF VERVLPGAIL
      181 RQLSDEEMNH YRRPFVNGGE DRRPTLSWPR NLPIDGEPAE VVALVNEYRS WLEETDMPKL
      241 FINAEPGAII TGRIRDYVRS WPNQTEITVP GVHFVQEDSP EEIGAAIAQF VRQLRSAAGV
//