LOCUS SIU01220.1 611 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable aspartyl-trna synthetase asps (aspartate--trna ligase) (asprs) (aspartic acid translase) protein. ACCESSION LT708304-2634 PROTEIN_ID SIU01220.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="aspS" /locus_tag="BQ2027_MB2602C" /note="Mb2602c, aspS, len: 611 aa. Equivalent to Rv2572c, len: 596 aa, from Mycobacterium tuberculosis strain H37Rv, (99.7% identity in 595 aa overlap). Probable aspS, aspartyl-tRNA synthetase (EC 6.1.1.12), equivalent to P36429|SYD_MYCLE|ML0501|MLCB1259.19 ASPARTYL-TRNA SYNTHETASE from Mycobacterium leprae (589 aa), FASTA scores: opt: 3534, E(): 1.8e-215, (87.85% identity in 592 aa overlap). Also highly similar to many e.g. O67589|SYD_AQUAE|AQ_1677 from Aquifex aeolicus (603 aa), FASTA scores: opt: 1829, E(): 8.2e-108, (47.5% identity in 598 aa overlap); O32038|SYD_BACSU from Bacillus subtilis (592 aa), FASTA scores: opt: 1732, E(): 1.1e-101, (46.25% identity in 597 aa overlap); P21889|SYD_ECOLI|TLS|B1866 from Escherichia coli strain K12 (590 aa), FASTA scores: opt: 1588, E(): 1.3e-92, (47.35% identity in 581 aa overlap); etc. Contains PS00179 Aminoacyl-transfer RNA synthetases class-II signature 1. BELONGS TO CLASS-II AMINOACYL-TRNA SYNTHETASE FAMILY. REMARK-M.bovis-M.tuberculosis: In Mycobacterium bovis, a single base deletion (t-*) omitting a stop codon, leads to a longer protein with a different COOH end compared to its homolog in Mycobacterium tuberculosis strain H37Rv (611 aa versus 596 aa). Protein product from Mb2602c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2602c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:Q7TYA6" /db_xref="InterPro:IPR002312" /db_xref="InterPro:IPR004115" /db_xref="InterPro:IPR004364" /db_xref="InterPro:IPR004365" /db_xref="InterPro:IPR004524" /db_xref="InterPro:IPR006195" /db_xref="InterPro:IPR012340" /db_xref="InterPro:IPR029351" /db_xref="UniProtKB/Swiss-Prot:Q7TYA6" BEGIN 1 MFVLRSHAAG LLREGDAGQQ VTLAGWVARR RDHGGVIFID LRDASGIAQV VFRDPQDTEV 61 LAQAHRLRAE FCVSVAGVVE IRPEGNANPE IATGEIEVNA TSLTVLGECA PLPFQLDEPA 121 GEELRLKYRY LDLRRDDPAA AIRLRSRVNA AARAVLARHD FVEIETPTIT RSTPEGARDF 181 LVPARLHPGS FYALPQSPQL FKQLLMVAGM ERYYQIARCY RDEDFRADRQ PEFTQLDMEM 241 SFVDAEDIIA ISEEVLTELW ALIGYRIPTP IPRIGYAEAM RRFGTDKPDL RFGLELVECT 301 DFFSDTTFRV FQAPYVGAVV MPGGASQPRR TLDGWQDWAK QRGHRGLAYV LVAEDGTLGG 361 PVAKNLTEAE RTGLADHVGA KPGDCIFFSA GPVKSSRALL GAARVEIANR LGLIDPDAWA 421 FVWVVDPPLF EPADEATAAG EVAVGSGAWT AVHHAFTAPK PEWEDRIESD TGSVLADAYD 481 IVCNGHEIGG GSVRIHRRDI QERVFAVMGL DKAEVEEKFG FLLEAFMFGA PPHGGIAFGW 541 DRTTALLAGM DSIREVIAFP KTGGGVDPLT DAPAPITAQQ RKESGIDAQP KRVQRHDQIF 601 AVTTSHPSVN K //