LOCUS SIU01213.1 533 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 LONG CONSERVED HYPOTHETICAL PROTEIN [FIRST PART] protein. ACCESSION LT708304-2627 PROTEIN_ID SIU01213.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB2595" /note="Mb2595, -, len: 533 aa. Equivalent to 5' end of Rv2566, len: 1140 aa, from Mycobacterium tuberculosis strain H37Rv, (99.6% identity in 531 aa overlap). Long conserved hypothetical protein, equivalent to O53120|ML2678 OR MLCB1913.12 HYPOTHETICAL PROTEIN from Mycobacterium leprae (1000 aa), FASTA scores: opt: 760, E(): 7.1e-38, (50.2% identity in 1128 aa overlap); and middle part equivalent to Q9ZB40 72.2 KDA PROTEIN (FRAGMENT) from Mycobacterium leprae (644 aa), FASTA scores: opt: 1017, E(): 1.5e-65, (45.65% identity in 655 aa overlap). Also highly similar to Q98HG6|MLL2877 HYPOTHETICAL PROTEIN from Rhizobium loti (Mesorhizobium loti) (1119 aa), FASTA scores: opt: 1413, E(): 3.7e-77, (52.4% identity in 1148 aa overlap); and N-terminus shows similarity with other proteins e.g. Q9HUN8|PA4926 HYPOTHETICAL PROTEIN from Pseudomonas aeruginosa (311 aa), FASTA scores: opt: 278, E(): 3e-09, (29.95% identity in 284 aa overlap); and upstream ORF Q50652|YP69_MYCTU|Rv2569c|MT2645|MTCY227.32 CONSERVED HYPOTHETICAL PROTEIN from Mycobacterium tuberculosis (314 aa), FASTA scores: opt: 252, E(): 1.1e-07, (28.9% identity in 315 aa overlap). Equivalent to AAK46955 from Mycobacterium tuberculosis strain CDC1551 (1156 aa) but shorter 16 aa. REMARK-M.bovis-M.tuberculosis: In Mycobacterium tuberculosis strain H37Rv, Rv2566 exists as a single gene. In Mycobacterium bovis, a frameshift due to a 2 bp deletion (gc-*) splits Rv2566 into 2 parts, Mb2595 and Mb2596. Mb2595 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="InterPro:IPR002931" /db_xref="InterPro:IPR013589" /db_xref="InterPro:IPR018667" /db_xref="InterPro:IPR038765" /db_xref="UniProtKB/TrEMBL:A0A1R3Y1K4" BEGIN 1 MPLRPTQVSS TGRTRCAGRS GVISSAAMSI KVALEHRTSY TFDRLVRVYP HIVRLRPAPH 61 SRTSIEAYSL RIEPADHFIN WQQDALGNFL ARLVFPNPMR QLRITVGLIA DLKVINPFDF 121 FIEDWAEIWP CAGMAYPKAL ADDLRPYLRP VDEDGDGSGP GELTQAWVRN FTVPDGTRTI 181 DFLVALNRAI NADVGYCVRM EPGVQTPDFT LRTGVGSCRD SAWLLVSILR QFGLAARFVS 241 GYLVQLASDI EALDGPSGPA ADFTDLHAWS EAYIPGAGWI GLDPTSGLLA GEGHIPLAAT 301 PHPASAAPIS GGTDVCDTVL EFSNTVTRVH EDPRVTLPYT DESWKTICEV GQRVDERLAA 361 ADVRLTVGGE PTFVSVDNQV AEEWRTAADG PHKRERASDL AARLKAVWAP QGLIHRGQGR 421 WYPGEPLPRW QIALYWRTDG RPLWTNDALL ADPWGAPPAD PVDDDAAYRV LAGIADGLGL 481 PISQVRPAYE DPLSRLAAAV RMPAGDPVES GDDLGCDTNP DTPTGRAALL APR //