LOCUS SIU01184.1 362 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 3-DEHYDROQUINATE SYNTHASE AROB protein. ACCESSION LT708304-2598 PROTEIN_ID SIU01184.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="aroB" /locus_tag="BQ2027_MB2567C" /note="Mb2567c, aroB, len: 362 aa. Equivalent to Rv2538c, len: 362 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 362 aa overlap). aroB, 3-dehydroquinate synthase (EC 4.2.3.4) (see citation below), equivalent to Q9CCS4|AROB_MYCLE|ML0518 3-DEHYDROQUINATE SYNTHASE from Mycobacterium leprae (361 aa), FASTA scores: opt: 2059, E(): 3.3e-117, (87.25% identity in 361 aa overlap). Also highly similar to many e.g. Q9KXQ6|AROB from Streptomyces coelicolor (363 aa), FASTA scores: opt: 1363, E(): 4e-75, (60.05% identity in 358 aa overlap); Q9X5D2|AROB_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (366 aa), FASTA scores: opt: 1154, E(): 1.7e-62, (50.95% identity in 359 aa overlap); P07639|AROB_ECOLI|B3389 from Escherichia coli strain K12 (362 aa), FASTA scores: opt: 771, E(): 2.4e-39, (40.6% identity in 345 aa overlap); etc. BELONGS TO THE DEHYDROQUINATE SYNTHASE FAMILY. Protein product from Mb2567c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2567c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P0A4Z5" /db_xref="InterPro:IPR016037" /db_xref="InterPro:IPR030960" /db_xref="InterPro:IPR030963" /db_xref="UniProtKB/Swiss-Prot:P0A4Z5" BEGIN 1 MTDIGAPVTV QVAVDPPYPV VIGTGLLDEL EDLLADRHKV AVVHQPGLAE TAEEIRKRLA 61 GKGVDAHRIE IPDAEAGKDL PVVGFIWEVL GRIGIGRKDA LVSLGGGAAT DVAGFAAATW 121 LRGVSIVHLP TTLLGMVDAA VGGKTGINTD AGKNLVGAFH QPLAVLVDLA TLQTLPRDEM 181 ICGMAEVVKA GFIADPVILD LIEADPQAAL DPAGDVLPEL IRRAITVKAE VVAADEKESE 241 LREILNYGHT LGHAIERRER YRWRHGAAVS VGLVFAAELA RLAGRLDDAT AQRHRTILSS 301 LGLPVSYDPD ALPQLLEIMA GDKKTRAGVL RFVVLDGLAK PGRMVGPDPG LLVTAYAGVC 361 AP //