LOCUS SIU01169.1 3069 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
FATTY ACID SYNTHASE FAS (FATTY ACID SYNTHETASE) protein.
ACCESSION LT708304-2583
PROTEIN_ID SIU01169.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/gene="fas"
/locus_tag="BQ2027_MB2553C"
/note="Mb2553c, fas, len: 3069 aa. Equivalent to Rv2524c,
len: 3069 aa, from Mycobacterium tuberculosis strain
H37Rv, (100.0% identity in 3069 aa overlap). Probable fas,
Fatty Acid Synthase (EC 2.3.1.-), equivalent to
Q9X7E2|FAS|ML1191 PUTATIVE TYPE I FATTY ACID SYNTHASE from
Mycobacterium leprae (3076 aa), FASTA scores: opt: 17484,
E(): 0, (85.8% identity in 3081 aa overlap). Also similar
to others e.g. Q04846|FAS|Q59497 from Corynebacterium
ammoniagenes (Brevibacterium ammoniagenes) (3104 aa),
FASTA scores: opt: 3981, E(): 5.5e-203, (49.8% identity in
3099 aa overlap); Q48926|FAS from Mycobacterium bovis
(2796 aa), FASTA scores: opt: 2098, E(): 3.9e-103, (59.7%
identity in 2862 aa overlap) (see first citation below);
P34731|FAS1_CANAL FATTY ACID SYNTHASE SUBUNIT BETA from
Candida albicans (Yeast) (2037 aa), FASTA scores: opt:
955, E(): 1.3e-42, (27.4% identity in 1926 aa overlap);
etc. Contains PS00017 ATP/GTP-binding site motif A
(P-loop), and PS00606 Beta-ketoacyl synthases active site.
Protein product from Mb2553c detected using shotgun mass
spectrometry and SWATH mass spectrometry. Mb2553c found to
be expressed during exponential growth in Sauton's minimal
media by RNA-sequencing (TPM > 20)."
/db_xref="GOA:A0A1R3Y1F3"
/db_xref="InterPro:IPR001227"
/db_xref="InterPro:IPR002539"
/db_xref="InterPro:IPR003965"
/db_xref="InterPro:IPR013565"
/db_xref="InterPro:IPR013785"
/db_xref="InterPro:IPR014030"
/db_xref="InterPro:IPR014031"
/db_xref="InterPro:IPR014043"
/db_xref="InterPro:IPR016035"
/db_xref="InterPro:IPR016039"
/db_xref="InterPro:IPR020801"
/db_xref="InterPro:IPR020841"
/db_xref="InterPro:IPR029069"
/db_xref="InterPro:IPR036291"
/db_xref="UniProtKB/TrEMBL:A0A1R3Y1F3"
/experiment="experimental evidence, no additional details
recorded"
BEGIN
1 MTIHEHDRVS ADRGGDSPHT THALVDRLMA GEPYAVAFGG QGSAWLETLE ELVSATGIET
61 ELATLVGEAE LLLDPVTDEL IVVRPIGFEP LQWVRALAAE DPVPSDKHLT SAAVSVPGVL
121 LTQIAATRAL ARQGMDLVAT PPVAMAGHSQ GVLAVEALKA GGARDVELFA LAQLIGAAGT
181 LVARRRGISV LGDRPPMVSV TNADPERIGR LLDEFAQDVR TVLPPVLSIR NGRRAVVITG
241 TPEQLSRFEL YCRQISEKEE ADRKNKVRGG DVFSPVFEPV QVEVGFHTPR LSDGIDIVAG
301 WAEKAGLDVA LARELADAIL IRKVDWVDEI TRVHAAGARW ILDLGPGDIL TRLTAPVIRG
361 LGIGIVPAAT RGGQRNLFTV GATPEVARAW SSYAPTVVRL PDGRVKLSTK FTRLTGRSPI
421 LLAGMTPTTV DAKIVAAAAN AGHWAELAGG GQVTEEIFGN RIEQMAGLLE PGRTYQFNAL
481 FLDPYLWKLQ VGGKRLVQKA RQSGAAIDGV VISAGIPDLD EAVELIDELG DIGISHVVFK
541 PGTIEQIRSV IRIATEVPTK PVIMHVEGGR AGGHHSWEDL DDLLLATYSE LRSRANITVC
601 VGGGIGTPRR AAEYLSGRWA QAYGFPLMPI DGILVGTAAM ATKESTTSPS VKRMLVDTQG
661 TDQWISAGKA QGGMASSRSQ LGADIHEIDN SASRCGRLLD EVAGDAEAVA ERRDEIIAAM
721 AKTAKPYFGD VADMTYLQWL RRYVELAIGE GNSTADTASV GSPWLADTWR DRFEQMLQRA
781 EARLHPQDFG PIQTLFTDAG LLDNPQQAIA ALLARYPDAE TVQLHPADVP FFVTLCKTLG
841 KPVNFVPVID QDVRRWWRSD SLWQAHDARY DADAVCIIPG TASVAGITRM DEPVGELLDR
901 FEQAAIDEVL GAGVEPKDVA SRRLGRADVA GPLAVVLDAP DVRWAGRTVT NPVHRIADPA
961 EWQVHDGPEN PRATHSSTGA RLQTHGDDVA LSVPVSGTWV DIRFTLPANT VDGGTPVIAT
1021 EDATSAMRTV LAIAAGVDSP EFLPAVANGT ATLTVDWHPE RVADHTGVTA TFGEPLAPSL
1081 TNVPDALVGP CWPAVFAAIG SAVTDTGEPV VEGLLSLVHL DHAARVVGQL PTVPAQLTVT
1141 ATAANATDTD MGRVVPVSVV VTGADGAVIA TLEERFAILG RTGSAELADP ARAGGAVSAN
1201 ATDTPRRRRR DVTITAPVDM RPFAVVSGDH NPIHTDRAAA LLAGLESPIV HGMWLSAAAQ
1261 HAVTATDGQA RPPARLVGWT ARFLGMVRPG DEVDFRVERV GIDQGAEIVD VAARVGSDLV
1321 MSASARLAAP KTVYAFPGQG IQHKGMGMEV RARSKAARKV WDTADKFTRD TLGFSVLHVV
1381 RDNPTSIIAS GVHYHHPDGV LYLTQFTQVA MATVAAAQVA EMREQGAFVE GAIACGHSVG
1441 EYTALACVTG IYQLEALLEM VFHRGSKMHD IVPRDELGRS NYRLAAIRPS QIDLDDADVP
1501 AFVAGIAEST GEFLEIVNFN LRGSQYAIAG TVRGLEALEA EVERRRELTG GRRSFILVPG
1561 IDVPFHSRVL RVGVAEFRRS LDRVMPRDAD PDLIIGRYIP NLVPRLFTLD RDFIQEIRDL
1621 VPAEPLDEIL ADYDTWLRER PREMARTVFI ELLAWQFASP VRWIETQDLL FIEEAAGGLG
1681 VERFVEIGVK SSPTVAGLAT NTLKLPEYAH STVEVLNAER DAAVLFATDT DPEPEPEEDE
1741 PVAESPAPDV VSEAAPVAPA ASSAGPRPDD LVFDAADATL ALIALSAKMR IDQIEELDSI
1801 ESITDGASSR RNQLLVDLGS ELNLGAIDGA AESDLAGLRS QVTKLARTYK PYGPVLSDAI
1861 NDQLRTVLGP SGKRPGAIAE RVKKTWELGE GWAKHVTVEV ALGTREGSSV RGGAMGHLHE
1921 GALADAASVD KVIDAAVASV AARQGVSVAL PSAGSGGGAT IDAAALSEFT DQITGREGVL
1981 ASAARLVLGQ LGLDDPVNAL PAAPDSELID LVTAELGADW PRLVAPVFDP KKAVVFDDRW
2041 ASAREDLVKL WLTDEGDIDA DWPRLAERFE GAGHVVATQA TWWQGKSLAA GRQIHASLYG
2101 RIAAGAENPE PGRYGGEVAV VTGASKGSIA ASVVARLLDG GATVIATTSK LDEERLAFYR
2161 TLYRDHARYG AALWLVAANM ASYSDVDALV EWIGTEQTES LGPQSIHIKD AQTPTLLFPF
2221 AAPRVVGDLS EAGSRAEMEM KVLLWAVQRL IGGLSTIGAE RDIASRLHVV LPGSPNRGMF
2281 GGDGAYGEAK SALDAVVSRW HAESSWAARV SLAHALIGWT RGTGLMGHND AIVAAVEEAG
2341 VTTYSTDEMA ALLLDLCDAE SKVAAARSPI KADLTGGLAE ANLDMAELAA KAREQMSAAA
2401 AVDEDAEAPG AIAALPSPPR GFTPAPPPQW DDLDVDPADL VVIVGGAEIG PYGSSRTRFE
2461 MEVENELSAA GVLELAWTTG LIRWEDDPQP GWYDTESGEM VDESELVQRY HDAVVQRVGI
2521 REFVDDGAID PDHASPLLVS VFLEKDFAFV VSSEADARAF VEFDPEHTVI RPVPDSTDWQ
2581 VIRKAGTEIR VPRKTKLSRV VGGQIPTGFD PTVWGISADM AGSIDRLAVW NMVATVDAFL
2641 SSGFSPAEVM RYVHPSLVAN TQGTGMGGGT SMQTMYHGNL LGRNKPNDIF QEVLPNIIAA
2701 HVVQSYVGSY GAMIHPVAAC ATAAVSVEEG VDKIRLGKAQ LVVAGGLDDL TLEGIIGFGD
2761 MAATADTSMM RGRGIHDSKF SRPNDRRRLG FVEAQGGGTI LLARGDLALR MGLPVLAVVA
2821 FAQSFGDGVH TSIPAPGLGA LGAGRGGKDS PLARALAKLG VAADDVAVIS KHDTSTLAND
2881 PNETELHERL ADALGRSEGA PLFVVSQKSL TGHAKGGAAV FQMMGLCQIL RDGVIPPNRS
2941 LDCVDDELAG SAHFVWVRDT LRLGGKFPLK AGMLTSLGFG HVSGLVALVH PQAFIASLDP
3001 AQRADYQRRA DARLLAGQRR LASAIAGGAP MYQRPGDRRF DHHAPERPQE ASMLLNPAAR
3061 LGDGEAYIG
//