LOCUS SIU01142.1 273 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE CITRATE (PRO-3S)-LYASE (BETA SUBUNIT) CITE (CITRASE) ( CITRATASE) (CITRITASE) (CITRIDESMOLASE) (CITRASE ALDOLASE) protein. ACCESSION LT708304-2556 PROTEIN_ID SIU01142.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="citE" /locus_tag="BQ2027_MB2526C" /note="Mb2526c, citE, len: 273 aa. Equivalent to Rv2498c, len: 273 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 273 aa overlap). Probable citE, citrate lyase, beta subunit (EC 4.1.3.6), similar to others e.g. Q9S3L3|CITE from Corynebacterium glutamicum (Brevibacterium flavum) (217 aa), FASTA scores: opt: 565, E(): 1.5e-28, (41.85% identity in 215 aa overlap); Q9HRM8|CITE|VNG0627G from Halobacterium sp. strain NRC-1 (303 aa), FASTA scores: opt: 535, E(): 1.5e-26, (41.65% identity in 276 aa overlap); Q9S2U9|SC4G6.02 from Streptomyces coelicolor (274 aa), FASTA scores: opt: 426, E(): 1e-19, (37.6% identity in 274 aa overlap); P77770|CILB_ECOLI from Escherichia coli (307 aa), FASTA scores: opt: 265, E(): 1.5e-10, (32.8% identity in 265 aa overlap); etc. Also similar to Rv3075c|MTCY22D7.06 from Mycobacterium tuberculosis, FASTA score: (35.2% identity in 264 aa overlap). Protein product from Mb2526c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2526c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y1E1" /db_xref="InterPro:IPR005000" /db_xref="InterPro:IPR011206" /db_xref="InterPro:IPR015813" /db_xref="InterPro:IPR040442" /db_xref="UniProtKB/TrEMBL:A0A1R3Y1E1" BEGIN 1 MNLRAAGPGW LFCPADRPER FAKAAAAADV VILDLEDGVA EAQKPAARNA LRDTPLDPER 61 TVVRINAGGT ADQARDLEAL AGTAYTTVML PKAESAAQVI ELAPRDVIAL VETARGAVCA 121 AEIAAADPTV GMMWGAEDLI ATLGGSSSRR ADGAYRDVAR HVRSTILLAA SAFGRLALDA 181 VHLDILDVEG LQEEARDAAA VGFDVTVCIH PSQIPVVRKA YRPSHEKLAW ARRVLAASRS 241 ERGAFAFEGQ MVDSPVLTHA ETMLRRAGEA TSE //