LOCUS SIU01139.1 393 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable branched-chain keto acid dehydrogenase e2 component bkdc protein. ACCESSION LT708304-2553 PROTEIN_ID SIU01139.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="bkdc" /locus_tag="BQ2027_MB2523C" /note="Mb2523c, pdhC, len: 393 aa. Equivalent to Rv2495c, len: 393 aa, from Mycobacterium tuberculosis strain H37Rv, (99.5% identity in 393 aa overlap). Probable pdhC, dihydrolipoamide S-acetyltransferase, e2 component (EC 2.3.1.12), similar to others e.g. Q9XA49|SCGD3.30c from Streptomyces coelicolor (491 aa) FASTA scores: opt: 615, E(): 1.2e-28, (36.45% identity in 491 aa overlap; several gaps); P19262|ODO2_YEAST|KGD2|YDR148C|YD8358.05c from Saccharomyces cerevisiae (Baker's yeast) (463 aa) FASTA scores: opt: 533, E(): 7.1e-24, (28.55% identity in 396 aa overlap); Q9HN75|DSA|VNG2219G from Halobacterium sp. strain NRC-1 (478 aa), FASTA scores: opt: 521, E(): E(): 3.7e-23, (30.25% identity in 486 aa overlap; in part); etc. BELONGS TO THE 2-OXOACID DEHYDROGENASE FAMILY. Protein product from Mb2523c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2523c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y1B8" /db_xref="InterPro:IPR000089" /db_xref="InterPro:IPR001078" /db_xref="InterPro:IPR004167" /db_xref="InterPro:IPR011053" /db_xref="InterPro:IPR023213" /db_xref="InterPro:IPR036625" /db_xref="UniProtKB/TrEMBL:A0A1R3Y1B8" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MSGEDSIRSF PVPDLGEGLQ EVTVTCWSVA VGDDVEINQT LCSVETAKAE VEIPSPYAGR 61 IVELGGAEGD VLKVGAELVR IDTGPTAVAQ PNGEGAVPTL VGYGADAAIE TSRRTSRPLA 121 APVVRKLAKE LAVDLAALQR GSGAGGVITR ADVLAAARGG VGAGPDVRPV HGVHARMAEK 181 MTLSHKEIPT AKASVEVICA ELLRLRDWFV SAAPEITPFA LTLRLLVIAL KHNVILNSTW 241 VDSGEGPQVH VHRGVHLGFG AATERGLLVP VVTDAQDKNT RELASRVAEL ITGAREGTLT 301 PAELRGSTFT VSNFGALGVD DGVPVINHPE AAILGLGAIK PRPVVVGGEV VARPTMTLTC 361 VFDHRVVDGA QVAQFMCELR DLIESPETAL LDL //