LOCUS       SIU01139.1               393 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 probable
            branched-chain keto acid dehydrogenase e2 component bkdc
            protein.
ACCESSION   LT708304-2553
PROTEIN_ID  SIU01139.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="bkdc"
                     /locus_tag="BQ2027_MB2523C"
                     /note="Mb2523c, pdhC, len: 393 aa. Equivalent to Rv2495c,
                     len: 393 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (99.5% identity in 393 aa overlap). Probable pdhC,
                     dihydrolipoamide S-acetyltransferase, e2 component (EC
                     2.3.1.12), similar to others e.g. Q9XA49|SCGD3.30c from
                     Streptomyces coelicolor (491 aa) FASTA scores: opt: 615,
                     E(): 1.2e-28, (36.45% identity in 491 aa overlap; several
                     gaps); P19262|ODO2_YEAST|KGD2|YDR148C|YD8358.05c from
                     Saccharomyces cerevisiae (Baker's yeast) (463 aa) FASTA
                     scores: opt: 533, E(): 7.1e-24, (28.55% identity in 396 aa
                     overlap); Q9HN75|DSA|VNG2219G from Halobacterium sp.
                     strain NRC-1 (478 aa), FASTA scores: opt: 521, E(): E():
                     3.7e-23, (30.25% identity in 486 aa overlap; in part);
                     etc. BELONGS TO THE 2-OXOACID DEHYDROGENASE FAMILY.
                     Protein product from Mb2523c detected using shotgun mass
                     spectrometry and SWATH mass spectrometry. Mb2523c found to
                     be expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing."
                     /db_xref="GOA:A0A1R3Y1B8"
                     /db_xref="InterPro:IPR000089"
                     /db_xref="InterPro:IPR001078"
                     /db_xref="InterPro:IPR004167"
                     /db_xref="InterPro:IPR011053"
                     /db_xref="InterPro:IPR023213"
                     /db_xref="InterPro:IPR036625"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3Y1B8"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MSGEDSIRSF PVPDLGEGLQ EVTVTCWSVA VGDDVEINQT LCSVETAKAE VEIPSPYAGR
       61 IVELGGAEGD VLKVGAELVR IDTGPTAVAQ PNGEGAVPTL VGYGADAAIE TSRRTSRPLA
      121 APVVRKLAKE LAVDLAALQR GSGAGGVITR ADVLAAARGG VGAGPDVRPV HGVHARMAEK
      181 MTLSHKEIPT AKASVEVICA ELLRLRDWFV SAAPEITPFA LTLRLLVIAL KHNVILNSTW
      241 VDSGEGPQVH VHRGVHLGFG AATERGLLVP VVTDAQDKNT RELASRVAEL ITGAREGTLT
      301 PAELRGSTFT VSNFGALGVD DGVPVINHPE AAILGLGAIK PRPVVVGGEV VARPTMTLTC
      361 VFDHRVVDGA QVAQFMCELR DLIESPETAL LDL
//