LOCUS       SIU01127.1               256 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            ENOYL-COA HYDRATASE ECHA14 (ENOYL HYDRASE) (UNSATURATED
            ACYL-COA HYDRATASE) (CROTONASE) protein.
ACCESSION   LT708304-2541
PROTEIN_ID  SIU01127.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="echA14"
                     /locus_tag="BQ2027_MB2511"
                     /note="Mb2511, echA14, len: 256 aa. Equivalent to Rv2486,
                     len: 256 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 256 aa overlap). Probable echA14,
                     enoyl-coA hydratase (EC 4.2.1.17), similar to others e.g.
                     P24162|ECHH_RHOCA2|FADB1 from Rhodobacter capsulatus
                     (Rhodopseudomonas capsulata) (257 aa), FASTA scores; opt:
                     453, E(): 3.8e-23, (39.4% identity in 259 aa overlap);
                     Q9ETY7|PACA|PAAG from Azoarcus evansii (273 aa), FASTA
                     scores: opt: 404, E(): 5.7e-17, (37.5% identity in 224 aa
                     overlap); P77467|PAAG_ECOLI from Escherichia coli (262
                     aa), FASTA scores: opt: 401, E(): 8.3e-17, (36.3% identity
                     in 259 aa overlap); etc. Contains PS00166 Enoyl-CoA
                     hydratase/isomerase signature. BELONGS TO THE ENOYL-COA
                     HYDRATASE/ISOMERASE FAMILY. Protein product from Mb2511
                     detected using shotgun mass spectrometry and SWATH mass
                     spectrometry. Mb2511 found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:P64019"
                     /db_xref="InterPro:IPR001753"
                     /db_xref="InterPro:IPR018376"
                     /db_xref="InterPro:IPR029045"
                     /db_xref="UniProtKB/Swiss-Prot:P64019"
BEGIN
        1 MAQYDPVLLS VDKHVALITV NDPDRRNAVT DEMSAQLRAA IQRAEGDPDV HAVVVTGAGK
       61 AFCAGADLSA LGAGVGDPAE PRLLRLYDGF MAVSSCNLPT IAAVNGAAVG AGLNLALAAD
      121 VRIAGPAALF DARFQKLGLH PGGGATWMLQ RAVGPQVARA ALLFGMCFDA ESAVRHGLAL
      181 MVADDPVTAA LELAAGPAAA PREVVLASKA TMRATASPGS LDLEQHELAK RLELGPQAKS
      241 VQSPEFAARL AAAQHR
//