LOCUS SIU01127.1 256 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE ENOYL-COA HYDRATASE ECHA14 (ENOYL HYDRASE) (UNSATURATED ACYL-COA HYDRATASE) (CROTONASE) protein. ACCESSION LT708304-2541 PROTEIN_ID SIU01127.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="echA14" /locus_tag="BQ2027_MB2511" /note="Mb2511, echA14, len: 256 aa. Equivalent to Rv2486, len: 256 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 256 aa overlap). Probable echA14, enoyl-coA hydratase (EC 4.2.1.17), similar to others e.g. P24162|ECHH_RHOCA2|FADB1 from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (257 aa), FASTA scores; opt: 453, E(): 3.8e-23, (39.4% identity in 259 aa overlap); Q9ETY7|PACA|PAAG from Azoarcus evansii (273 aa), FASTA scores: opt: 404, E(): 5.7e-17, (37.5% identity in 224 aa overlap); P77467|PAAG_ECOLI from Escherichia coli (262 aa), FASTA scores: opt: 401, E(): 8.3e-17, (36.3% identity in 259 aa overlap); etc. Contains PS00166 Enoyl-CoA hydratase/isomerase signature. BELONGS TO THE ENOYL-COA HYDRATASE/ISOMERASE FAMILY. Protein product from Mb2511 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2511 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P64019" /db_xref="InterPro:IPR001753" /db_xref="InterPro:IPR018376" /db_xref="InterPro:IPR029045" /db_xref="UniProtKB/Swiss-Prot:P64019" BEGIN 1 MAQYDPVLLS VDKHVALITV NDPDRRNAVT DEMSAQLRAA IQRAEGDPDV HAVVVTGAGK 61 AFCAGADLSA LGAGVGDPAE PRLLRLYDGF MAVSSCNLPT IAAVNGAAVG AGLNLALAAD 121 VRIAGPAALF DARFQKLGLH PGGGATWMLQ RAVGPQVARA ALLFGMCFDA ESAVRHGLAL 181 MVADDPVTAA LELAAGPAAA PREVVLASKA TMRATASPGS LDLEQHELAK RLELGPQAKS 241 VQSPEFAARL AAAQHR //