LOCUS SIU01106.1 268 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE DNA GLYCOSYLASE protein. ACCESSION LT708304-2520 PROTEIN_ID SIU01106.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB2491C" /note="Mb2491c, -, len: 268 aa. Equivalent to Rv2464c, len: 268 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 268 aa overlap). Possible DNA glycosylase (EC 3.2.2.-), showing some similarity to several other DNA glycosylases e.g. Q9F308|SCC80.11c PUTATIVE DNA REPAIR HYDROLASE (FRAGMENT) from Streptomyces coelicolor (306 aa), FASTA scores: opt: 894, E(): 6.1e-51, (51.05% identity in 282 aa overlap); O50606|MUTM|FPG_THETH FORMAMIDOPYRIMIDINE-DNA GLYCOSYLASE (EC 3.2.2.23) from Thermus aquaticus (267 aa), FASTA scores: opt: 342, E(): 4.6e-15, (32.4% identity in 250 aa overlap); Q9RCW5|SCM10.34c PUTATIVE FORMAMIDOPYRIMIDINE-DNA GLYCOSYLASE from Streptomyces coelicolor (287 aa), FASTA scores: opt: 321, E(): 1.1e-13, (29.35% identity in 259 aa overlap); etc. Identical to AAK46839|MT2539 FORMAMIDOPYRIMIDINE-DNA GLYCOSYLASE from Mycobacterium tuberculosis strain CDC1551. Also similar to other M. tuberculosis DNA glycosylases e.g. MTCY71.37 (32.9% identity in 277 aa overlap). BELONGS TO THE FPG FAMILY. Protein product from Mb2491c detected using SWATH mass spectrometry. Mb2491c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P64159" /db_xref="InterPro:IPR000214" /db_xref="InterPro:IPR010663" /db_xref="InterPro:IPR010979" /db_xref="InterPro:IPR012319" /db_xref="InterPro:IPR015886" /db_xref="InterPro:IPR015887" /db_xref="InterPro:IPR035937" /db_xref="UniProtKB/Swiss-Prot:P64159" BEGIN 1 MPEGHTLHRL ARLHQRRFAG APVSVSSPQG RFADSASALN GRVLRRASAW GKHLFHHYVG 61 GPVVHVHLGL YGTFTEWARP TDGWLPEPAG QVRMRMVGAE FGTDLRGPTV CESIDDGEVA 121 DVVARLGPDP LRSDANPSSA WSRITKSRRP IGALLMDQTV IAGVGNVYRN ELLFRHRIDP 181 QRPGRGIGEP EFDAAWNDLV SLMKVGLRRG KIIVVRPEHD HGLPSYLPDR PRTYVYRRAG 241 EPCRVCGGVI RTALLEGRNV FWCPVCQT //