LOCUS SIU01104.1 466 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE TRIGGER FACTOR (TF) PROTEIN TIG protein. ACCESSION LT708304-2518 PROTEIN_ID SIU01104.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="tig" /locus_tag="BQ2027_MB2489C" /note="Mb2489c, tig, len: 466 aa. Equivalent to Rv2462c, len: 466 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 466 aa overlap). Probable tig, trigger factor (TF), a chaperone protein, equivalent to Q9CBY2|ML1481 POSSIBLE MOLECULAR CHAPERONE from Mycobacterium leprae (469 aa), FASTA scores: opt: 2171, E(): 7.2e-113, (70.1% identity in 468 aa overlap). Also similar to oyher trigger factors from several organisms e.g. Q9F314|SCC80.05c from Streptomyces coelicolor (468 aa), FASTA scores: opt: 1224, E(): 1.7e-60, (41.8% identity in 469 aa overlap); Q9K8F3|TIG_BACHD from Bacillus halodurans (431 aa), FASTA scores: opt: 675, E(): 3.6e-30, (28.5% identity in 421 aa overlap); P22257|TIG_ECOLI from Escherichia coli (432 aa), FASTA scores: opt: 493, E(): 4.2e-20, (23.35% identity in 433 aa overlap); etc. BELONGS TO THE FKBP-TYPE PPIASE FAMILY, TIG SUBFAMILY. Protein product from Mb2489c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2489c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:Q7TYJ1" /db_xref="InterPro:IPR005215" /db_xref="InterPro:IPR008880" /db_xref="InterPro:IPR008881" /db_xref="InterPro:IPR027304" /db_xref="InterPro:IPR036611" /db_xref="InterPro:IPR037041" /db_xref="UniProtKB/Swiss-Prot:Q7TYJ1" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MKSTVEQLSP TRVRINVEVP FAELEPDFQR AYKELAKQVR LPGFRPGKAP AKLLEARIGR 61 EAMLDQIVND ALPSRYGQAV AESDVQPLGR PNIEVTKKEY GQDLQFTAEV DIRPKISLPD 121 LSALTVSVDP IEIGEDDVDA ELQSLRTRFG TLTAVDRPVA VGDVVSIDLS ATVDGEDIPN 181 AAAEGLSHEV GSGRLIAGLD DAVVGLSADE SRVFTAKLAA GEHAGQEAQV TVTVRSVKER 241 ELPEPDDEFA QLASEFDSID ELRASLSDQV RQAKRAQQAE QIRNATIDAL LEQVDVPLPE 301 SYVQAQFDSV LHSALSGLNH DEARFNELLV EQGSSRAAFD AEARTASEKD VKRQLLLDAL 361 ADELQVQVGQ DDLTERLVTT SRQYGIEPQQ LFGYLQERNQ LPTMFADVRR ELAIRAAVEA 421 ATVTDSDGNT IDTSEFFGKR VSAGEAEEAE PADEGAARAA SDEATT //